Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal SAB4200774-100UL

Monoclonal Anti-Shiga Toxin 1, B Subunit (mouse IgG1 isotype) is derived from the 13C4 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a purified Shiga-like toxin from E. coli H30. Shiga toxins consist of two polypeptides, an A chain and a B chain non-covalently associate with an apparent stoichiometry of one A and five B chains to form the holotoxin.
The Shiga toxins are a family of related protein toxins secreted by certain types of bacteria. Shiga toxin (Stx) is produced by Shigella dysenteriae; whereas, the Shiga-like toxins, Stx1 and Stx2, with a few known isoforms, are secreted by specific strains of Escherichia coli named Shiga-toxin-producing E. coli (STEC) such as E. coli O157:H7. The latter may cause bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications.
Specificity
Monoclonal Anti-Shiga Toxin 1, B Subunit antibody specifically recognizes the B subunit of Shiga holotoxin.
purified Shiga-like toxin from E.coli H30
Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal has been used in:

• immunoblotting
• flow cytometry
• immunoprecipitation

Biochem/physiol Actions
Shiga-toxin (Stx) produced by E. coli O157:H7 may cause bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications. All the Stx isoforms have similar structure and mechanism of action. The catalytic A subunit has RNA N-glycosidase activity that inhibits eukaryotic protein synthesis. The B subunits form a pentamer, which recognizes and binds to the functional cell-surface receptor globotriaosylceramide [Gb3, Gala(1-4)-Galb (1-4)-Glcb1-ceramide]. Gb3 is overexpressed in membranes of numerous tumor cells, Therefore, STxB binding to Gb3 receptors may be useful for cell-specific vectorization, labeling, and imaging purposes.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
This product is for R&D use only, not for drug, household, or other uses.

Quality Level	200
biological source	mouse
antibody form	purified from hybridoma cell culture
antibody product type	primary antibodies
clone	13C4, monoclonal
description	Research area: Microbiome
form	buffered aqueous solution
mol wt	~7 kDa
species reactivity	E. coli
concentration	~1.0 mg/mL
application(s)	flow cytometry: 2.5-5 µg/test using human RAMOS cells pretreated with recombinant Shiga toxin 1, B subunit","immunoblotting: 1-2 µg/mL using purified recombinant Shiga Toxin 1-B subunit produced in E. coli (using PVDF membrane)","immunoprecipitation (IP): suitable
isotype	IgG1
UniProt accession no.	Q7BQ98,
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable