Anti-Shiga Toxin 1, B Subunit-FITC antibody, Mouse monoclonal SAB4200799-100UL

NACRES:

NA.41

Monoclonal Anti-Shiga Toxin 1, B Subunit-FITC (mouse IgG1 isotype) is derived from the hybridoma 13C4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The Shiga toxins are a family of related protein toxins secreted by certain types of bacteria. Shiga toxin (Stx) is produced by Shigella dysenteriae; whereas, the Shiga-like toxins, Stx1 and Stx2, with a few known isoforms, are secreted by specific strains of Escherichia coli named Shiga-toxin-producing E. coli (STEC) such as E. coli O157:H7. Stx1 is identical to Stx and the Stx2 isoforms shares 60% similarity with STX. Howevere, the toxin structure is conserved. Shiga toxins consist of two polypeptides: A and B.
Specificity
Monoclonal Anti-Shiga Toxin 1, B Subunit -FITC recognizes the B subunit of Shiga holotoxin.
Purifed Shiga-like toxin from E. coli H30.
Anti-Shiga Toxin 1, B Subunit-FITC antibody, Mouse monoclonal may be used in flow cytometry.
Biochem/physiol Actions
The STX catalytic A subunit has RNA N-glycosidase activity that inhibits eukaryotic protein synthesis. The B subunits form a pentamer, which recognizes and binds to the functional cell-surface receptor globotriaosylceramide [Gb3, Gal (1-4)-Gal (1-4)-Glc1-ceramide]. Shiga toxins are implicated in bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications. Gb3 is overexpressed in membranes of numerous tumor cells, therefore, STxB binding to Gb3 receptors may be useful in cancer cell imaging.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4 and 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2–8 °C protected from light for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. Protect from prolonged exposure to light.
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified from hybridoma cell culture
antibody product type	primary antibodies
clone	13C4, monoclonal
description	Research area: Microbiome
form	buffered aqueous solution
species reactivity	E. coli
storage condition	protect from light
concentration	~1 mg/mL
application(s)	flow cytometry: 2-4 µg/test using human RAMOS cells pretreated with recombinant Shiga toxin 1, B subunit
isotype	IgG1
conjugate	FITC conjugate
UniProt accession no.	Q7BQ98,
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3