Anti-Pseudomonas Exotoxin A-Peroxidase antibody produced in rabbit SAB4200828-1VL

Pseudomonas aeruginosa is a Gram negative bacterium that rarely cause diseases in humans but certain populations, such as cystic fibrosis (CF) or burn wounds patients, are prone to such infections.
Pseudomonas Exotoxin A (PE) is the most potent virulence factor secreted by P. aeruginosa, it is composed of three structural domains, N-terminal domain (I) responsible for the toxin binding to its host cell receptor, middle domain (II) has a role in toxin translocation across the membrane, and C-terminal domain (III) has ADP-ribosylation activity. PE is considered as a selective agent for the elimination of specific cell populations due to its toxin ADP-ribosylation activity resulting in irreversibly shut down of protein synthesis. To reduce the adverse effects of natural PE, Mutated PE was used in several attempts to develop recombinant toxin-antibody or cytokines fusion fragments for therapeutic application including cancer immunotherapy.
Purified Exotoxin A from Pseudomonas aeruginosa
The antibody may be used in various immunochemical techniques including ELISA.
Physical form
Supplied as a lyophilized powder.
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
antibody form	IgG fraction of antiserum
antibody product type	primary antibodies
clone	polyclonal
description	Research area: Microbiome
form	lyophilized powder
species reactivity	Pseudomonas aeruginosa
concentration	~4 mg/mL
application(s)	direct ELISA: 1:10,000-1:20,000 (is recommended using 1 µg/ml Pseudomonas Exotoxin A for coating.)
conjugate	peroxidase conjugate
UniProt accession no.	P11439,
shipped in	dry ice
storage temp.	20°C
Gene Information	Pseudomonas aeruginosa PAO1 ... toxA(877850)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable