- Производитель:
- Sigma-Aldrich
NACRES: | NA.41 |
Кат. номер |
SAB4200825-25UL |
SAB4200825-100UL |
Specificity
Anti-o-spanin antibody specifically recognizes the Outer membrane lipoprotein subunit o-spanin from E. coli and Shigella flexneri and does not cross react with Proteus mirabilis.
Biochem/physiol Actions
Spanins are conserved among lambdoid phages and comprise the final step of lysis in lambda phage infections of E. coli. These proteins form heterotetramer complex composed of two homodimers linked by homotypic intermolecular disulfide bonds, span the periplasm and bridge the cell envelope thus causing fusion of the inner and outer membranes and disruption of the bacterium membrane.The DLP12 lysis genes play an important, yet to be identified role in the bacterial cell wall maintenance and biofilm formation. This was shown using mutated strains lacking essD, ybcS and rzpD/rzoD that were unable to form biofilms and had significant lower aggregation as compared with wild-type. These finding suggests that defective prophage genes are affecting host cell physiology contribute to host fitness and survival, biofilm dispersion, enhanced biofilm traits such as antibiotic resistance, attachment, phage tolerance and metabolite recycling.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12
hours.
Disclaimer
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source | rabbit |
antibody form | affinity isolated antibody |
antibody product type | primary antibodies |
clone | polyclonal |
description | Research area: Microbiome |
form | buffered aqueous solution |
mol wt | ~20 kDa |
species reactivity | E. coli |
packaging | antibody small pack of 25 µL |
concentration | ~1 mg/mL |
application(s) | immunoblotting: 1:500-1:1000 using E. coli lysate. |
UniProt accession no. | P58041, |
shipped in | dry ice |
storage temp. | 20°C |
Gene Information | Escherichia coli K12 ... rzoD(1450242) |
Flash Point F | Not applicable |
Flash Point C | Not applicable |
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