Hanahans Broth (SOB Medium)

Кат. номер

H8032-500G

H8032-500G-PW

Hannahans Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation. The 0.4% dextrose composition provides the E. coli carbon and energy needed to mend perforations and for replication.
Hanahans Broth is recommended for use in cultivation of recombinant strains of Escherichia coli. It is suitable for the preparation of competent E. coli., often with the addition of 0.4% dextrose. It has also been used for the preparation of C. necator H16 competent cells. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
Hannahans Broth (SOB) powder provides:

• Standard formulation
• An inexpensive alternative to liquid

Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Casein enzymic hydrolysate and yeast extract supply the necessary nitrogenous compounds and growth factors. Potassium chloride and sodium chloride provide an isotonic environment for the cells. Magnesium sulfate aids DNA replication.

Quality Level	100,
grade	for molecular biology
description	recommended for cultivation of recombinant E. coli.
sterility	non-sterile
form	powder
composition	KCl, 0.186 g/L , MgSO4, 2.4 g/L , NaCl, 0.5 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L
pH range	6.8 - 7.2 (2.8% solution)
Featured Industry	Food and Beverages
storage temp.	room temp
suitability	nonselective for Escherichia coli, nonselective for coliforms

Personal Protective Equipment	Eyeshields,, Gloves,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable