m-Heterotrophic Plate Count Broth MHA000P2S

eCl@ss:	32160801
NACRES:	NA.74

Heterotrophs are organisms including bacteria, yeasts and moulds that require an external source of organic carbon for growth. The heterotrophic plate count (HPC), formerly known as the standard plate count, is a procedure for estimating the number of live heterotrophic bacteria in water.{14} This test can provide useful information about water quality and supporting data on the significance of coliform test results. Heterotrophic Plate Count Broth can be employed for the determination of bacteria by the membrane filter method. Peptic digest of animal tissue is the source of nutrients for organisms, which are not highly fastidious. Gelatin is utilized by microorganisms through a proteolytic mechanism. The addition of glycerol to the basal medium provides a source of carbon and energy
m-HPC broth is used in the recovery of stressed heterotrophic bacteria found in various types of water. Also suitable for water samples with low counts. Beer, Bottled Water, Cider, Soft Drink, Sport Drink and Wine. The media finds its application in Clinical, Cosmetics, Environmental Analysis, Food and Beverage and Industrial QC.
50 pk in 2 mL plastic ampoules
2 mL plastic ampoules
Incubation Time: 48–72 hrs
Standard Methods for the Examination of Water and Wastewater
Incubation Temperature: 30–35 °C
Prior to use, warm media at 30-50 °C until liquefied. Do not microwave.
m-HPC
Colonies appear clear to creamy white, some may produce pigment
Target Microorganisms: Total Count
QC organisms
E. coli (ATCC 25922)
S. epidermidis (ATCC 14990)
MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	2 to 10 °C
Quality Level	100,
form	liquid
shelf life	12 mo.
feature	ready-to-use
packaging	pkg of 2 mL, pack of 50 tests
application(s)	cell quantification: suitable (total viable count (TVC)), microbiology: suitable, water monitoring: suitable
color	clear tan to light amber
input	sample type bottled water sample type beer sample type beverage(s) sample type sport drink sample type soft drink sample type food(s) sample type cosmetics sample type cider sample type wine
pH	7.1±0.2 (25.0 °C)
Featured Industry	Clinical Cosmetics Environmental Food and Beverages Industrial QC
compatibility	for use with MF-method, for use with Microfil®, for use with MicropreSure®, for use with Milliflex®
shipped in	wet ice
storage temp.	2-8°C
suitability	nonselective for

WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable