m-Green Yeast and Mold Broth MHA000P2M

eCl@ss:	32160801
NACRES:	NA.74

m-Green Yeast and Mold Broth is a relatively more complex formula compared to other media used for isolation of fungi and yeast.{10} This formulation is rich in nutrient which allows excellent fungal growth. Bacterial growth is inhibited by an acidic pH. Enzymatic digest of casein and enzymatic digest of animal tissue provide nitrogen, carbon, and amino acids in m-Green yeast and mold Broth. Yeast extract is the vitamin source. Dextrose is an energy source for metabolism of fungi. Potassium phosphate is a buffering agent. Magnesium sulphate, Thiamine, and Diastase (a mixture containing amylolytic (starch) enzymes) provide essential ions, minerals, and nutrients. Bromocresol Green is the pH indicator, facilitating visualization and counting of fungal colonies. The colonies are green due to the diffusion of bromocresol green into the colonies (alkaline reaction). Acidic end products from the colonies diffuse into the medium, further reducing the pH and causing the dye to turn yellow (acid reaction). Yeast produces green, opaque large colonies. Mold colonies are green and filamentous.
m-Green yeast and mold Broth is used for the testing soft drinks and other beverages like beer, bottled water, cider, soft drink, sport drink and wine for growth of yeast and mold.
50 pk in 2 mL plastic ampoules
2 mL plastic ampoules
Incubation Temperature: 28–32 °C
Incubation Time: 48–72 hrs
m-Green Yeast and Mold
Target Microorganisms: Yeast and mold (selection by pH)
QC organisms
S. cerevisiae (ATCC 9763)
A. niger (ATCC 16404)
Yeast produce green, opaque large colonies. Mold colonies are green and filamentous
MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	2 to 10 °C
Quality Level	100,
form	liquid
shelf life	12 mo.
feature	ready-to-use
packaging	pkg of 2 mL, pack of 50 tests
application(s)	microbiology: suitable, water monitoring: suitable
color	green
input	sample type sport drink sample type beer sample type soft drink sample type cosmetics sample type cider sample type beverage(s) sample type bottled water sample type wine sample type beverage(s) sample type food(s)
pH	4.6±0.2 (25.0 °C)
Featured Industry	Clinical Cosmetics Environmental Food and Beverages Industrial QC
compatibility	for use with MF-method, for use with Microfil®, for use with MicropreSure®, for use with Milliflex®
shipped in	wet ice
storage temp.	2-8°C

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable