ECC ChromoSelect Selective Agar

MDL number:	MFCD01634645
NACRES:	NA.85

Кат. номер

85927-VAR

85927-26.5G-F

85927-500G-F

85927-VAR-F

ECC ChromoSelect Selective Agar is a selective medium recommended for the simultaneous detection of Escherichia coli and coliforms in water and food samples. The ingredients help even the sublethally injured coliforms to grow rapidly, Tergitol™ inhibits Gram-positive as well as some Gram-negative bacteria other than coliforms. The chromogenic mixture contains two chromogenic substrates as Salmon-GAL and X-glucuronide. The enzyme -D-galactosidase produced by coliforms cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme -D-glucuronidase produced by E. coli, cleaves X-glucuronide. E. coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction. To confirm E. coli, add a drop of Kovacs reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.
Suspend 26.5 g in 1 litre distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species add 5 mg Cefsulodin (Cat. No. 22126) for surface and pour plate method and 10 mg Cefsulodin for membrane filter technique. Mix well.
Suspend 26.5 g in 1 liter of distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species, add 5 mg of cefsulodin for surface and pour plate method and 10 mg of cefsulodin for membrane filter technique. Mix well.
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TERGITOL is a trademark of The Dow Chemical Company ("Dow") or an affiliated company of Dow

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 10.0 g/L , casein enzymic hydrolysate, 3.3 g/L , chromogenic mixture, 0.43 g/L , disodium hydrogen phosphate, 1 g/L , peptone, special, 6.0 g/L , sodium chloride, 2 g/L , sodium dihydrogen phosphate, 0.6 g/L , sodium pyruvate, 1 g/L , sorbitol, 1 g/L , Tergitol™ 7, 0.15 g/L , tryptophan, 1 g/L
Торговая марка	NutriSelect® Plus
application(s)	bioburden testing: suitable, microbiology: suitable (microbial identification), water monitoring: suitable
final pH	6.8±0.2 (25 °C)
Featured Industry	Agriculture Environmental Food and Beverages
suitability	selective and differential for Citrobacter spp., selective and differential for Enterobacter spp., selective and differential for Escherichia coli, selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for coliforms, selective and differential for enterobacteriaceae

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable