De Man, Rogosa and Sharpe Broth MHA00MRS2

eCl@ss:	32160801
NACRES:	NA.74

MRS Broth is used for the enrichment, cultivation and isolation of all species of Lactobacillus. The MRS media formulation was developed by de Man, Rogosa and Sharpe {23} to replace the tomato juice medium and the meat extract tomato juice medium. It is a medium supporting good growth of lactobacilli in general, even those strains which have shown poor growth in existing media, like strains of L. brevis and L. fermenti. The MRS culture media contain polysorbate (Tween 80), acetate, magnesium and manganese which are known to act as special growth factors for Lactobacilli as well as a rich nutrient base. As these media show a very low degree of selectivity Pediococcus and Leuconostoc species as well as other secondary bacteria may grow on them. Most of the accompanying microflora can be inhibited by thallium acetate, sorbic acid, acetic acid, sodium nitrite, cycloheximide and polymyxin. Lactobacillus species appear as small, white, creamy colonies. Saccharomyces species appear as large, white, creamy colonies.{24}
MRS Broth is used for final product testing or in-process testing of beverages for the contamination by lactobacilli, L. brevis and L. fermenti in Beer, Bottled Water, Cider, Soft Drink and Sport Drink. This media finds application in Clinical, Cosmetics and Food and Beverage industry.
50 pk in 2 mL plastic ampoules
MRS
QC organisms
L. casei (ATCC 393)
Lactobacillus species appear as small, white, creamy colonies. Saccharomyces species appear as large, white, creamy colonies
MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	2 to 10 °C
Quality Level	100,
form	liquid
shelf life	12 mo.
feature	ready-to-use
packaging	ampule of 2 mL, pack of 50 tests
application(s)	microbiology: suitable, water monitoring: suitable
color	clear red-brown
input	sample type beverage(s), sample type food(s) sample type bottled water sample type wine sample type cider sample type cosmetics sample type beer sample type soft drink sample type sport drink
pH	6.5±0.2 (25.0 °C)
Featured Industry	Clinical Cosmetics Environmental Food and Beverages Industrial QC
compatibility	for use with MF-method, for use with Microfil®, for use with MicropreSure®, for use with Milliflex®
shipped in	wet ice
storage temp.	2-8°C
suitability	Lactobacillus brevis, Lactobacillus fermenti, lactic acid bacteria

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable