Metabolism Assay Kits
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Nickel Assay Kit MAK027-1KT
NACRES: NA.84 General descriptionNickel acts as a cofactor for several enzymes, including some ureases, carbon monoxide dehydrogenases (methane forming enzymes which reduce CO2 to CH4), and some hydrogenases. Nickel forms complexes with sulfhydryl compounds with significant absorbance in the UV/visible region in the presence of other ions.
Nickel acts as a cofactor for several enzymes, including some ureases, carbon monoxide dehydrogenases (methane forming enzymes which reduce CO2 to CH4), and some hydrogenases. Nickel forms complexes with sulfhydryl compounds with significant absorbance in the UV/visible region in the presence of other ions.
In the Nickel Assay kit, Ni2+ reacts with 2-mercaptoethanol in borate buffer to form a complex with strong absorbance bands from 300 to 600 nm. Fe2+ and Co2+ interfere with the assay; therefore, extra steps must be taken to subtract the interference in order to determine the correct nickel concentration in mixed samples. Other ions tested (Mn2+, Cu2+, and Zn2+) do not interfere with the assay and presumably no other ionic species will be present in high enough concentration to interfere with the reaction. The assay is a simple method of quantifying Ni2+ in a variety of samples, which gives a linear range of 2–50 nmole nickel in samples containing <25 nmole of Co2+ ions.SuitabilitySuitable for quantifying nickel concentrations in a variety of samples.PrincipleIn the Nickel Assay kit, Ni2+ reacts with 2-mercaptoethanol in borate buffer to form a complex with strong absorbance bands from 300 to 600 nm. Fe2+ and Co2+ interfere with the assay; therefore, extra steps must be taken to subtract the interference in order to determine the correct nickel concentration in mixed samples. Other ions tested (Mn2+, Cu2+, and Zn2+) do not interfere with the assay and presumably no other ionic species will be present in high enough concentration to interfere with the reaction. The assay is a simple method of quantifying Ni2+ in a variety of samples, which gives a linear range of 2–50 nmole nickel in samples containing <25 nmole of Co2+ ions.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. 2-8°C
Safety Informationpictograms GHS07,GHS08 signalword Danger hcodes H317 - H350i - H373 - H412 pcodes P201 - P273 - P280 - P308 + P313 RIDADR NONH for all modes of transport Узнать цену -
NIR Mitochondrion Membrane Potential Kit MAK149-1KT
NACRES: NA.25 General descriptionMitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis, collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.SuitabilityThis kit is suitable for the detection of Mitochondrial Membrane Potential in mammalian cells and for screening apoptosis inhibitors and activators using microplate readers.PrincipleNIR Mitochondrion Membrane Potential Kit is optimized for the detection of the loss of the MMP in cells. The cationic hydrophobic mitochondrial potential dye accumulates in normal mitochondria, most likely due to the mitochondrial potential, resulting in an increase in fluorescence (λex = 640/λem = 680 nm). In apoptotic cells, MMP collapse results in decreased fluorescence. This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators.Параметрыusage sufficient for 500 fluorometric tests (near infrared fluorescence, microplate readers) detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Nitric Oxide Synthase Inhibitor Screening Kit MAK323-1KT
General descriptionNitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO is the oxidation product of arginine by nitric oxide synthase (NOS) and is involved in host defense development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Inhibition of NOS has the potential to produce diverse biological effects, particularly in the cardiovascular system. Simple, direct and non-radioactive procedures for measuring NOS are becoming popular in research and drug discovery.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for inhibitor screening and evaluation of nitric oxide synthase inhibitors.PrincipleThe Nitric Oxide Synthase Inhibitor Screening Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric
Safety Informationpictograms GHS05,GHS07 signalword Warning hcodes H290 - H302 - H315 - H319 pcodes P234 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338 Узнать цену -
Nitrite Assay Kit (Griess Reagent) MAK367-1KT
General descriptionNitrogen-based ions, nitrite and nitrate, are found in almost every living organism. Furthermore, endogenous Nitrite levels are found in mammals and can also be obtained from dietary sources. In humans, nitrite is further metabolized to Nitric Oxide and other reactive nitrogen species (nitrogen oxides). The Nitrate-Nitrite-NO biochemical pathway is well known for its participation in cell signaling, hypoxiadependent response and regulation of blood flow. Recent studies suggest the reduction of Nitrite to Nitrogen Oxygen in the mitochondria. Specifically, myoglobin and xanthine oxidoreductase could generate NO under hypoxic conditions leading to mitochondrial respiration.SuitabilityThe kit is suitable for the detection of Nitric Oxide in animal tissues (liver, kidney, etc.), cell culture (adherent or suspension cells), serum, plasma and urine.PrincipleThe Nitrite Assay Kit utilizes the Griess Reagent, a classic protocol for the estimation of nitrite. In the assay, nitrite is reduced to Nitrogen Oxide using Griess Reagent I. Then, Nitrogen Oxide reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at A540 nm. The one-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.Параметрыusage sufficient for 200 colorimetric tests detection method colorimetric storage temp. 2-8°C
Safety Informationpictograms GHS05 signalword Danger hcodes H290 - H314 pcodes P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
NNMT Inhibitor Screening Assay Kit MAK299-1KT
NACRES: NA.84 General descriptionN′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy.SuitabilitySuitable for screening/studying/characterizing potential inhibitors of N′-Nicotinamide Methyltransferase (NNMT).PrincipleThis NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using the Thiol Detecting Probe, generating an enhanced fluorescence signal (λex = 392 nm/ λem = 482 nm). In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.Параметрыdetection method fluorometric storage temp. −70°C
Safety Informationpictograms GHS02,GHS05,GHS07,GHS08 signalword Danger hcodes H226 - H290 - H312 - H314 - H317 - H361fd - H412 pcodes P201 - P210 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 + P310 RIDADR UN 3316 9 Flash Point F 84.2 °F Flash Point C 29 °C Узнать цену -
Oxalate Assay Kit MAK315-1KT
NACRES: NA.84 General descriptionOxalate or Oxalic Acid is a metabolic breakdown product of the Krebs Cycle in eukaryotes, and the glyoxylate cycle in other microorganisms. It can be found in the urine of humans and other mammals. Oxalate concentration can be used as a measure of kidney function where a high level of oxalate is an indicator for kidney stones which are primarily made of the insoluble salt calcium oxalate. Measuring oxalate is more accurate than measuring calcium as a marker for kidney stones because calcium is excreted at high concentrations even in normal urine.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the quantitative determination of oxalate (oxalic acid) in urine, animal and plant tissue samples.PrincipleSimple, direct and high-throughput assays for measuring oxalate concentration find wide applications. This oxalate assay kit uses a single Working Reagent that combines the oxalate oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 595 nm is directly proportional to oxalate in the sample.Параметрыdetection method colorimetric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Oxalate Decarboxylase Activity Assay Kit MAK214-1KT
EC Number: 200-550-3 NACRES: NA.84 General descriptionOxalate Decarboxylase (OXDC, EC 4.1.1.2) catalyzes the conversion of oxalate to formate and carbon dioxide in the presence of molecular oxygen. OXDC-responsive proteins are involved in the regulation of metabolism and stress responses in plants.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the detection of Oxalate Decarboxylase activity in samples such as plants and fungi.PrincipleThe Oxalate Decarboxylase Activity Assay Kit provides a simple and high throughput adaptable procedure for measuring OXDC activity in a variety of plant and fungal tissues. OXDC activity is determined by generating a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of oxalate decarboxylase is the amount of enzyme required to generate 1.0 μmole of formate per minute at pH 5 at 37 °C.Параметрыdetection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H302 + H312 pcodes P264 - P270 - P280 - P301 + P312 - P302 + P352 + P312 - P362 + P364 RIDADR NONH for all modes of transport Узнать цену -
Oxalic Acid Colorimetric Assay Kit MAK179-1KT
EC Number: 200-550-3 NACRES: NA.84 General descriptionOxalic acid (Oxalate) is a strong dicarboxylic acid that occurs in several plants and vegetables. In animals, it is either incorporated from the diet or produced during ascorbic acid and glyoxylic acid metabolism. Oxalic acid is not metabolized and is excreted in the urine. Hence, it can act as a marker for glyoxylic and ascorbic acid metabolism. Furthermore, oxalic acid has been studied as a pathogenic factor for Sclerotinia sclerotiorum that inhibits the oxidative burst response in host plants. It is also known to alleviate chilling injury in peach fruit. Elevated levels of oxalic acid (oxalate) have been associated with the formation of kidney stones. Hence, oxalate measurements can be used for the diagnosis and monitoring of renal stones.ApplicationOxalic acid colorimetric assay kit has been used in 24-hour urinary oxalate level (24HUOL) evaluation in an animal model.SuitabilitySuitable for the detection of oxalate (oxalic acid) in biological samples including animal and plant tissue.PrincipleThe Oxalic Acid Assay Kit provides a simple and sensitive procedure for measuring oxalic acid in variety of samples including animal and plant tissue. In this assay, oxalic acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (450 nm) product, proportional to the oxalate present.Параметрыdetection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H302 + H312 pcodes P264 - P270 - P280 - P301 + P312 - P302 + P352 + P312 - P362 + P364 RIDADR NONH for all modes of transport Узнать цену -
Oxaloacetate Assay Kit MAK070-1KT
NACRES: NA.84 General descriptionOxaloacetate (OAA) is an intermediate of both the TCA cycle and gluconeogenesis. It precedes citrate which is formed by the citrate synthase-mediated condensation of acetyl-CoA and OAA. OAA is also formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl-CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase. The Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples.ApplicationOxaloacetate Assay Kit has been used to determine the concentration of oxaloacetate in samples.SuitabilitySuitable for animal tissues, cell culture (adherent or suspension cells), serum and saliva.PrincipleIn this kit, oxaloacetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the oxaloacetate present. Typical detection ranges for this kit are 2–10 nmole (colorimetric) and 0.2–1 nmole (fluorometric).Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C Узнать цену -
PAH (p-Aminohippuric Acid) Assay Kit MAK101-1KT
NACRES: NA.25 General descriptionp-Aminohippuric acid (PAH), a derivative of hippuric acid, is used in the measurement of renal plasma flow (RPF). Renal plasma flow measures the volume of plasma, which enters the kidney in a given period of time. To determine effective renal plasma flow, PAH is measured in a plasma sample (PPAH) and a urine sample (UPAH). The volume of urine flow (V) is also required.SuitabilitySuitable for measuring PAH (p-Aminohippuric Acid) in plasma and urine samplesPrincipleThe PAH (p-Aminohippuric Acid) Assay kit can be used for measuring PAH in a plasma and urine samples. PAH reacts with 4-(dimethylamino)cinnamaldehyde (DACA) to generate a compound, which can be detected spectrophotometrically at 550 nm.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05,GHS07,GHS09 signalword Danger hcodes H314 - H335 - H410 pcodes P261 - P273 - P280 - P305 + P351 + P338 - P310 - P501 Target Organs Respiratory system RIDADR UN 3316 9 Узнать цену -
PDH Activity Assay Kit MAK183-1KT
NACRES: NA.84 General descriptionPyruvate dehydrogenase (PDH) is a mitochondrial enzyme that catalyzes the conversion of pyruvate to acetyl-CoA and CO2, and also links the tricarboxylic acid (TCA) and glycolysis pathways. The enzyme is inhibited by phosphorylation and activated by dephosphorylation. Mutations in PDH have been linked to pyruvate dehydrogenase deficiency (causing lactic acidosis and neurologic dysfunctions) and Leigh syndrome. PDH has also been implicated in oncogene-induced senescence. PDH measurements can provide insights into metabolic functions and oncogenesis.ApplicationPDH Activity Assay Kit has been used to determine the activity of pyruvate dehydrogenase.SuitabilitySuitable for the detection of PDH activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.PrinciplePyruvate dehydrogenase activity is determined using a coupled enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of pyruvate dehydrogenase is the amount of enzyme that will generate 1.0 mmole of NADH per minute at 37 °C.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. 20°C Gene Information human ... PDHA1(5160), PDHA2(5161), PDHB(5162), PDHX(8050)
mouse ... PDHA1(18597), PDHA2(18598), PDHB(68263), PDHX(27402)
rat ... PDHA1(29554), PDHA2(117098), PDHB(289950), PDHX(311254)
Safety Informationpictograms GHS05,GHS07 signalword Danger hcodes H314 - H317 pcodes P261 - P272 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
PEG Virus Precipitation Kit MAK343-1KT
General descriptionVirus is typically produced at a low titer. It often needs to be concentrated for storage and/or for use in further applications.SuitabilitySuitable to concentrate retroviruses, baculoviruses, lentiviruses, and phages etc. in cell culture medium or environmental samples.Параметрыusage sufficient for 200 preparations storage temp. −20°C
Safety InformationFlash Point F Not applicable Flash Point C Not applicable Узнать цену -
PEP Colorimetric/Fluorometric Assay Kit MAK102-1KT
EC Number: 215-185-5 NACRES: NA.84 General descriptionPhospho(enol)pyruvate (PEP) in an intermediate in carbohydrate metabolism involved in glycolysis and gluconeogeneis. In glycolysis, PEP is metabolized by pyruvate kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.ApplicationPEP Colorimetric/Fluorometric Assay Kit has been used to measure the concentrations of phosphoenolpyruvate.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the measurement of phosphoenolpyruvate (PEP) in tissue, cells and plant extractsPrinciplePEP concentration is determined by a coupled enzyme assay, in which PEP is converted to ATP and pyruvate, resulting in a colorimetric (570 nm)/fluorometric (ex = 535/em = 587 nm) product, proportional to the PEP present.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 pcodes P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312 RIDADR UN 3316 9 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Peroxidase Activity Assay Kit MAK092-1KT
NACRES: NA.84 General descriptionPeroxidase (EC 1.11.1.7) is an enzyme found broadly in biological systems that utilizes hydrogen peroxide in the oxidation of various substrates. The Peroxidase Activity Assay Kit provides a simple and direct procedure for measuring peroxidase activity in a variety of biological samples.ApplicationPeroxidase Activity Assay Kit has been used to quantify the activity of peroxidase.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the determination of peroxidase activity in cell culture supernatant, serum, plasma, urine, and other biological fluids.PrinciplePeroxidase catalyzes the reaction between H2O2 and the probe, resulting in a colorimetric (570 nm)/fluorometric (ex = 535/em = 587 nm) product, proportional to the peroxidase activity present. One unit of peroxidase is defined as the amount of enzyme that reduces1.0 µmole of H2O2 per minute at 37°C.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 pcodes P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312 RIDADR NA 1993 / PGIII Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Peroxide Assay Kit MAK311-1KT
NACRES: NA.84 General descriptionPeroxide (e.g. hydrogen peroxide H2O2), a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.ApplicationPeroxide Assay Kit has been used to measure hydrogen peroxide level in rat tissue homogenates.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium.PrincipleThe Peroxide Assay Kit provides a simple and high-throughput adaptable assay for quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium. This assay utilizes the chromogenic Fe3+ xylenol orange reaction, in which a purple complex is formed when Fe2+ is oxidized to Fe3+ by peroxides present in the sample, generating a colorimetric (585 nm) result, proportional to the level of peroxide present. The optimized formulation reduces interference by substances in the raw samples. A concentration of 1 µM H2O2 equals 34 ng/mL or 34 ppb. The kit has a detection range of 0.2–30 µM (7–1,020 ng/mL) H2O2 in a 96 well format.Параметрыdetection method colorimetric storage temp. 2-8°C
Safety Informationpictograms GHS05 signalword Warning hcodes H290 - H315 - H319 pcodes P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 RIDADR UN 2796 8 / PGII Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Phenolic Compounds Assay Kit (Colorimetric) MAK365-1KT
General descriptionPhenolic compounds are phytochemical secondary metabolites found abundantly in dietary and medicinal plants, vegetables and fruits. Major types of phytochemical phenolic compounds include simple phenolic acids (such as gallic acid and vanillic acid), flavonoids (such as catechin), stilbenoids, lignans and various highly complex polyphenols (proanthocyanidins and tannins). These compounds play an important role in plant defense against ultraviolet radiation, serve as a deterrent to herbivores and also act as signaling molecules in ripening and other plant growth processes. Both simple phenolic acids and complex polyphenols are found in high concentrations in foods and beverages such as berries, vegetables, cereals, coffee, tea and wine. Phenolic compounds, being antioxidants, have been increasingly studied in dietary sources, due to their protective effects against cardiovascular diseases, cancer and neurodegenerative diseases. Studies have also shown dietary polyphenols to possess antimicrobial, anti-inflammatory and anti-allergic properties.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the measurement of phenolic compounds in fruits, vegetables, beverages (e.g. tea, wine, coffee), food products, plant extracts and natural or herbal products.PrincipleThe Phenolic Compounds Assay Kit provides a quick, sensitive and selective method for measuring the total amount of phenolic compounds in various biological samples. In this assay, phenolic compounds couple with diazonium salts under alkaline conditions to form a stable diazo chromophore, detectable by absorbance at 480 nm. Unlike the classical Folin-Ciocalteu protocol for measuring phenolic compounds, this assay is not affected by non-phenolic reducing substances such as sulfites, reducing sugars or ascorbic acid. The assay is high-throughput adaptable and can detect concentrations of phenolic compounds as low as 0.02 mM catechin equivalents (CEs) from a variety of plant and food-based samples.Параметрыusage sufficient for 200 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS02,GHS07 signalword Danger hcodes H225 - H319 pcodes P210 - P305 + P351 + P338 Flash Point F 49.5 °F Flash Point C 9.7 °C Узнать цену -
Phenylalanine Assay Kit MAK005-1KT
NACRES: NA.84 General descriptionPhenylalanine is a non-polar essential amino acid. In the liver, phenylalanine is converted to tyrosine, which is a precursor for multiple compounds including L-DOPA and melanin. Defects in the activity of phenylalanine hydroxylase result in the inherited metabolic disorder phenylketonuria (PKU). PKU results in the build up of phenylalanine and phenylalanine metabolites, and can result in growth defects and mental retardation if not treated.SuitabilitySuitable for L-phenylalanine detection in cell and tissue culture supernatants, serum, and other biological samplesPrinciplePhenylalanine concentration is determined by a coupled enzyme assay, which results in the deamination of phenylalanine and the production of NADH which reacts with the probe resulting in a fluorescent (ex = 535 nm/em = 587 nm) product, proportional to the phenylalanine present.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Phosphate Assay Kit MAK308-1KT
NACRES: NA.84 General descriptionPhosphate is one of the most abundant minerals in the human body and contains about 1 % of total body weight. Phosphate homeostasis is maintained by the several regulators such as parathyroid hormone (PTH), calcitriol and phosphatonins. Maintenance of phosphate homeostasis is essential for various biological processes, such as energy metabolism, cell signaling, regulation of protein synthesis, skeletal development and bone integrity. An adequate level of phosphate is needed for the process of apoptosis of mature chondrocytes in the growth plate. Phosphate deficiency is associated with the bone pathology and clinical illness.ApplicationThis kit may be used to measure the liberation of free orthophosphate in the following applications:- Phosphatase Assays: liberation of phosphate from peptide, protein, or small molecule substrate.
- Lipase Assays: liberation of phosphate from phospholipids
- Nucleoside Triphosphatase Assays: liberation of phosphate from nucleoside triphosphates (ATP, GTP, TTP, CTP etc).
- Quantitation of phosphate in phospholipids, proteins and DNAs, etc.
- Drug Discovery: high-throughput screen for phosphatase inhibitors.
Features and BenefitsCompatible with high-throughput handling systems.
Reagent very stable: Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits.- High sensitivity and wide detection range: detection of as little of 20 pmoles of phosphate and useful range between 0.4–50 M phosphate.
- Fast and convenient: homogeneous “mix-andmeasure” assay allows quantitation of free phosphate within 30 minutes.
- Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes, or microplates, on spectrophotometers and plate readers.
- Robust and amenable to HTS: Z factors of 0.7–0.9 are observed in 96 well plates. Can be readily automated on HTS liquid handling systems.
SuitabilitySuitable for phosphate determination in enzyme reactions with free phosphate releases.PrincipleThe Phosphate Assay Kit is based on a proprietary formulation of the malachite green dye. The kit reagent forms a blue colored complex with free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be performed in tubes, cuvettes or multi-well plates. The assays can be conveniently executed in 96-well plates for high-throughput screening of enzyme inhibitors.Параметрыusage sufficient for 500 colorimetric tests detection method colorimetric storage temp. 2-8°C
Safety Informationpictograms GHS05 signalword Warning hcodes H290 pcodes P234 - P390 RIDADR UN 3264 8 / PGIII Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Phosphate Colorimetric Kit MAK030-1KT
General descriptionPhosphate is an essential component in living organisms and contributes to a variety of biological functions, including structural roles within nucleic acids, cellular membranes and bone. Phosphate is also important in the transport of cellular energy, nucleic acid metabolism and signal transduction. Hyperphosphatemia, a condition of excess phosphate levels in the blood, can lead to calcification of organs and interference with usage of other inorganic ions, such as iron, calcium, magnesium and zinc.
Phosphate is an essential component in living organisms and contributes to a variety of biological functions, including structural roles within nucleic acids, cellular membranes, and bone. Phosphate is also important in the transport of cellular energy, nucleic acid metabolism, and signal transduction. Hyperphosphatemia, a condition of excess phosphate levels in the blood, can lead to calcification of organs and interference with usage of other inorganic ions, such as iron, calcium, magnesium, and zinc.
The Phosphate Colorimetric Assay Kit provides a simple and direct procedure for measuring phosphate in a variety of samples. Phosphate reacts with a chromogenic complex, which results in a colorimetric (650 nm) product proportional to the amount of phosphate present. Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of 0.1 nmol, can be directly determined.ApplicationPhosphate colorimetric kit has been used to measure the phosphate concentration in a variety of samples.SuitabilitySuitable for measuring phosphate levels in a variety of samples including serum and urinePrincipleThe Phosphate Colorimetric Assay Kit provides a simple and direct procedure for measuring phosphate in a variety of samples. Phosphate reacts with a chromogenic complex, which results in a colorimetric (650 nm) product proportional to the amount of phosphate present. Phosphate concentrations between 1 μM and 1 mM, with a lower limit of detection of 0.1 nmol, can be directly determined.Параметрыusage sufficient for 500 colorimetric tests detection method colorimetric storage temp. room temp
Safety Informationpictograms GHS05 signalword Warning hcodes H290 RIDADR NONH for all modes of transport Узнать цену -
Phosphate Fluorometric Kit MAK031-1KT
NACRES: NA.84 General descriptionPhosphate is an essential component in living organisms and contributes to a variety of biological functions, including structural roles within nucleic acids, cellular membranes and bone. Phosphate is also important in the transport of cellular energy, nucleic acid metabolism and signal transduction. Hyperphosphatemia, a condition of excess phosphate levels in the blood, can lead to calcification of organs and interference with usage of other inorganic ions, such as iron, calcium, magnesium and zinc.SuitabilitySuitable for the detection of inorganic phosphate (Pi) produced through reactions involving ATPases, GTPases, 5-nucleotidase, protein phosphatases, acidand alkaline phosphatases, phosphorylase, etc. from a variety of samplesPrincipleThe Phosphate Fluorometric Assay Kit provides a simple and direct procedure for measuring phosphate in a variety of samples. Phosphate reacts with maltose to produce glucose in the presence of an enzyme. The glucose is subsequently oxidized and reacts with a probe, resulting in a fluorometric (ex = 535/em = 587 nm) product proportional to the amount of phosphate present. This kit has a linear range of detection between 50-250 pmoles.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety InformationFlash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Phosphatidylcholine Assay Kit MAK049-1KT
NACRES: NA.84 General descriptionPhosphatidylcholine (PC) accounts for more than 50% of the phospholipids composing mammalian plasma membranes and is particularly enriched in the extracellular leaflet. PC is synthesized in the liver and is required for lipoprotein assembly and secretion. PC is cleaved by phospholipase D, which hydrolyses the choline head, producing the signaling intermediate phosphatidic acid.ApplicationPhosphatidylcholine Assay Kit has been used to determine the concentration of phosphatidylcholine in samples.SuitabilitySuitable for the measurement of phosphatidylcholine in a variety of biological samplesPrincipleIn this assay, PC concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (ex = 535/em = 587 nm) product, proportional to the PC present.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety InformationFlash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Phosphatidylethanolamine Assay Kit (Fluorometric) MAK361-1KT
General descriptionPhosphatidylethanolamine (PE), also known as cephalin is the second most abundant phospholipid in animal and plant tissues and is present on the cytoplasmic side of the plasma membrane. Phosphatidylethanolamine is a neutral phospholipid consisting of a phosphatidyl group ester linked to an ethanolamine molecule. Its functions include membrane fission/fusion, maintenance of membrane curvature and stabilization of membrane proteins, since it can form hydrogen bonds with proteins through an ionizable amine group. It acts as a chaperone during assembly of membrane proteins and aids in their translocation from the cytoplasm to the membrane. It is also involved in secretion of very low-density lipoproteins in the liver.SuitabilitySuitable for the measurement of Phosphatidylethanolamine content in lipid extracts from cells and tissues.PrincipleThe Phosphatidylethanolamine assay kit is a microplate based enzymatic assay for the quantitation of PE in cells and tissues. PE Converter hydrolyses PE to an intermediate, which converts a colorless probe to a fluorescent product via enzymatic reaction (λex = 535 nm/λem = 587 nm). The intermediate formed through PE converter hydrolysis is specific to phosphatidylethanolamine. No other phospholipids (i.e. phosphatidylcholine, phosphatidylinositol or phosphatidic acid) will be detected, making the kit highly specific. The assay kit can detect as low as 0.2 nmol per well.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −70°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 pcodes P261 - P284 - P501 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Phosphatidylserine Assay Kit (Fluorometric) MAK371-1KT
General descriptionPhosphatidylserine (PS) is a glycerophospholipid consisting of a phosphatidyl group attached to L-serine via a phosphodiester linkage. PS is a critical component of the cellular plasma membrane and accounts for 2-15% of plasma membrane lipid composition, depending on the cell or tissue type. The highest concentrations of PS are found in neuronal tissues, which are critical for maintaining conduction velocity in myelinated neurons, as well as for higher order cognitive skills such as learning and memory. In normal, healthy cells, PS is held in the inner membrane surface (facing the cytosol) by the lipid transporter protein flippase. However, in apoptotic cells, PS molecules ‘shuffle between the inner and outer plasma membrane monolayers. When PS molecules flip to the extracellular (outer) surface of the cell membrane, they act as a signal for macrophages to engulf and digest the (apoptotic) cell.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the measurement of phosphatidylserine concentration in lipid extracts such as soft tissue homogenates (i.e. liver, brain, etc.) and cultured cell lysates (adherent or suspension cells).PrincipleThe Phosphatidylserine Assay Kit allows for quantification of PS in lipid extracts of cell and tissue lysates. The assay is based on the enzymatic cleavage of PS to yield phosphatidic acid and L-serine, which is subsequently metabolized and reacts with a probe to form a stable fluorophore at λex = 538 nm/λem = 587 nm. The assay is selective for PS (other phospholipids such as phosphatidylcholine, phosphatidylethanolamine or phosphatidic acid do not interfere). The kit is high-throughput ready and highly sensitive, detecting as little as 50 pmole/well of PS (5 μM in a 10 μL sample volume).Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS08,GHS09 signalword Danger hcodes H334 - H411 pcodes P261 - P273 - P284 - P391 - P501 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Phosphoenolpyruvate Carboxykinase Activity Assay Kit (Colorimetric) MAK408-1KT
General descriptionPhosphoenolpyruvate Carboxykinase (PEPCK) is an enzyme which belongs to the lyase family. In the presence of GTP, it catalyzes the reversible conversion of oxaloacetate (OAA) into phosphoenolpyruvate (PEP), GDP and CO2. In humans, two isoforms of PEPCK are found: cytosolic form (PEPCK-C, also called PCK1) and mitochondrial form (PEPCK-M). PEPCK-C is a rate-controlling step in gluconeogenesis. Recent studies found abnormal concentrations of PEPCK in diabetic mice. Therefore, accurate measurement of Phosphoenolpyruvate Carboxykinase activity is valuable for both mechanistic and therapeutic studies.Features and BenefitsPhosphoenolpyruvate Carboxykinase Activity Assay Kit:- Compatible with high-throughput handling systems.
- Sensitive, detecting than 10 µU of Phosphoenolpyruvate Carboxykinase activity per sample.
SuitabilityThe kit is suitable for the measurement of Phosphoenolpyruvate Carboxykinase activity in various samples including tissue (rat liver, kidney, heart etc.) and adherent/suspension cells (HeLa, Jurkat, HEK 293 cells etc.). As well as the analysis of the gluconeogenesis pathway.PrincipleIn this assay, Phosphoenolpyruvate Carboxykinase is coupled with a set of enzymes that convert phosphoenolpyruvate and carbonate into a series of intermediates and hydrogen peroxide, which in turn, reacts with a probe and is converted generating a colorimetric signal (A570 nm). The color intensity is directly proportional to the amount of active Phosphoenolpyruvate Carboxykinase present in samples.ПараметрыQuality Level 100, detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS07,GHS08 signalword Danger hcodes H317 - H334 - H335 pcodes P261 - P271 - P272 - P280 - P284 - P302 + P352 Target Organs Respiratory system Узнать цену -
Phosphofructokinase (PFK) Activity Colorimetric Assay Kit MAK093-1KT
NACRES: NA.84 General descriptionPhosphofructokinase (PFK) catalyzes the third step of glycolysis, the conversion of fructose-6-phosphate to fructose-1,6-diphosphate, the rate limiting step of glycolysis. PFK activity is highly regulated by multiple cofactors in addition to post-translational modifications and can be used to measure glycolytic flux in tissues. Deficiencies in PFK activity can result in the glycogen storage disease, glycogenosis type VII (Tarui′s disease), which is a glycogen storage disease.ApplicationPhosphofructokinase (PFK) Activity Colorimetric Assay Kit has been used to measure the activity of phosphofructokinase.SuitabilitySuitable for the measurement of phosphofructokinase activity, analysis of glucose metabolism and cell signaling and screening anti-cancer drugs in animal tissues (liver, brain, heart, muscles etc.) and cell culture (adherent or suspension cells).PrincipleThe Phosphofructokinase Colorimetric Assay kit provides a simple and direct procedure for measuring PFK activity in a variety of samples. PFK activity is determined by a coupled enzyme assay, in which fructose-6-phosphate and ATP is converted to fructose-1,6-diphosphate and ADP by PFK. The ADP is converted by the enzyme mix to AMP and NADH. The resulting NADH reduces a colorless probe resulting in a colorimetric (450 nm) product proportional to the PFK activity present. One unit of PFK is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 7.4 at 37 °C.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. 20°C Gene Information human ... PFKL(5211), PFKM(5213), PFKP(5214)
mouse ... PFKL(18641), PFKM(18642), PFKP(56421)
rat ... PFKL(25741), PFKM(65152), PFKP(60416)
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Phosphoglucomutase Colorimetric Assay Kit MAK105-1KT
NACRES: NA.84 General descriptionPhosphoglucomutase (PGM) is an enzyme crucial for the interconversion of glucose-1-phosphate and glucose-6-phosphate. In glycogenolysis, PGM converts glucose-1-phosphate into glucose-6-phosphate for use in either the glycolytic pathway or pentose phosphate pathway. When blood glucose levels are high, PGM will convert excess glucose-6-phosphate (formed from glucose by the action of hexokinase) to glucose-1-phosphate.
Deficiency of phosphoglucomutase leads to glycogen storage disease.SuitabilitySuitable for the measurement of phosphoglucomutase activity, analysis of glucose metabolism and cell signaling and screening anti-diabetic drugs in animal tissues (muscle, liver, heart, kidney etc.), cell culture (adherent or suspension cells) and plasma.PrincipleThe Phosphoglucomutase Colorimetric Assay kit provides a simple and direct procedure for measuring PGM activity in a variety of samples. PGM activity is determined by a coupled enzyme assay in which glucose-1-phosphate is converted by PGM to glucose-6-phosphate. Glucose-6-phosphate is subsequently oxidized to form a product, which reacts with a probe generating a colorimetric (450 nm) product proportional to the PGM activity present. One unit of PGM is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
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Phosphoglucose Isomerase Colorimetric Assay Kit MAK103-1KT
General descriptionPhosphoglucose Isomerase (PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate in the second step of glycolysis and is involved in glucogenesis. PGI is also referred to as Autocrine Motility Factor (AMF) and is secreted by cancer cells in order to stimulate metastasis. Deficiencies in PGI activity can lead to hemolytic anemia.SuitabilitySuitable for the measurement of phosphoglucose isomerase activity, analysis of glucose metabolism and cell signaling and screening anti-diabetic drugs in animal tissues (liver, brain, heart, kidney etc.), cell culture (adherent or suspension cells), bacteria, yeast, fish etc.PrincipleThe Phosphoglucose Isomerase Colorimetric Assay kit provides a simple and direct procedure for measuring PGI activity in a variety of samples. PGI activity is determined by a coupled enzyme assay in which fructose-6-phosphate is converted by PGI to glucose-6-phosphate. Glucose-6-phosphate is subsequently oxidized to form a product, which reacts with a probe generating a colorimetric (450 nm) product proportional to the PGI activity present. One unit of PGI is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05,GHS08 signalword Danger hcodes H314 - H334 pcodes P260 - P280 - P284 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) MAK412-1KT
General descriptionPhosphoglycerate Dehydrogenase (PHGDH) is the first enzyme that participates in the L-serine synthesis pathway. PHGDH catalyzes the reversible reaction that converts 3-phosphoglycerate into 3 phosphohydroxyglycerate and the NADH-dependent reduction of a-ketoglutarate into D-2-hydroxyglutarate (D-2HG). In humans, PHGDH deficiency causes severe health problems including congenital microcephaly, psychomotor retardation, and intractable seizure. Recent studies have shown increased PHGDH activity in a wide variety of cancers, particularly in breast and melanoma tumors. Therefore, the measurement of PHGDH activity has become of great importance in searching potential therapeutic agents against cancer.Features and BenefitsPhosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit:- Compatible with high-throughput handling systems.
- Includes Positive Control.
- Sensitive, detecting phosphoglycerate dehydrogenase activity less than 0.1 mU per sample.
SuitabilityThe kit is suitable for the measurement of PHGDH activity in various tissues (liver and kidney, etc.) and cells (adherent or suspension cells) and for the analysis of de novo L-serine biosynthesis.PrincipleThe Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay kit provides a quick and easy method for monitoring PHGDH activity in a variety of samples. In this kit, phosphoglycerate dehydrogenase converts 3-phosphoglycerate and NAD into 3-phosphohydroxyglerate and NADH respectively. The oxidation of NADH reduces a probe generating a strong, stable absorbance signal at A450 nm.ПараметрыQuality Level 100, detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05,GHS07 signalword Danger hcodes H314 - H335 pcodes P261 - P271 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P305 + P351 + P338 Target Organs Respiratory system Узнать цену -
Phosphoglycerate Mutase Activity Assay Kit (Colorimetric/Fluorometric) MAK424-1KT
General descriptionPhosphoglycerate Mutase (PGAM) is an important enzyme in the glycolytic pathway that catalyzes the reversible conversion of 3-phosphoglycerate to 2-phosphoglycerate. It plays an important role in regulating glycolysis and anabolic activity to promote cancer cell proliferation. Several studies reported that PGAM is upregulated in many human cancers. Inhibition of this enzyme could lead to tumor cell death thereby making PGAM an attractive therapeutic target.Features and BenefitsPhosphoglycerate Mutase Activity Assay Kit:- Sensitive method to detect PGAM activity as low as 0.05 mU in a variety of samples.
SuitabilityThe kit is suitable for the measurement of PGAM activity in various tissues (liver, muscle, heart etc.) and cells (adherent or suspension cells), the analysis of glycolytic metabolism in different sample types, the mechanistic studies of various cancers and for the screening of anti-cancer drugs.PrincipleThe kit utilizes the ability of an active PGAM to catalyze the conversion of 3-Phosphoglycerate to 2-Phosphoglycerate (2-PG), which is subsequently used to generate an intermediate product. The intermediate product then stoichiometrically reacts with a probe to generate color (A570 nm) or fluorescence (ex = 535 nm/em = 587 nm).ПараметрыQuality Level 100, detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS07,GHS08 signalword Danger hcodes H315 - H317 - H319 - H334 - H335 pcodes P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338 Target Organs Respiratory system Узнать цену -
Phospholipase D Activity Assay Kit MAK137-1KT
NACRES: NA.84 General descriptionPhospholipase D (PLD) catalyzes the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and choline. It is ubiquitously expressed and is implicated in immune function and cancer inhibition. Abnormalities in PLD expression have been associated with human cancers such as breast, renal, gastric and colorectal cancer.ApplicationSuitable for the detection of phospholipase D activity in biological samples. This kit can be used to study the effects of various compounds on phospholipase D activity.
Phospholipase D (PLD) Activity Assay Kit has been used to measure PLD activity in the liver and lysosomal fractions of the mice liver.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.PrincipleIn this assay, PLD hydrolyzes phosphatidylcholine to choline, which is determined using choline oxidase resulting in a colorimetric (570 nm)/fluorometric (λex = 530/λem = 585 nm) product, proportional to the PLD activity in the sample. Unit definition: one unit of PLD catalyzes the formation of 1 mmole choline per minute under the assay conditions (pH 7.4).Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. 20°C Gene Information human ... PLD1(5337), PLD2(5338), PLD3(23646), PLD4(122618), PLD5(200150), PLD6(201164)
mouse ... PLD1(18805), PLD2(18806), PLD3(18807), PLD4(104759), PLD5(319455), PLD6(194908)
rat ... PLD1(25096), PLD2(25097), PLD3(361527), PLD4(362792), PLD5(289270), PLD6(287366)
Safety Informationpictograms GHS05,GHS09 signalword Danger hcodes H315 - H318 - H410 pcodes P273 - P280 - P302 + P352 - P305 + P351 + P338 + P310 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Phospholipid Assay Kit MAK122-1KT
NACRES: NA.84 General descriptionPhospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline.
In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (ex = 530/em = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 µM for colorimetric assays and 0.6 - 20 µM for fluorometric assays.ApplicationPhospholipid assay kit has been used in biochemical analysis and phospholipid quantitation.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the detection of phospholipids in biological samplesPrincipleIn this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (ex = 530/em = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 µM for colorimetric assays and 0.6 - 20 µM for fluorometric assays.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric shipped in dry ice storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 - H412 pcodes P261 - P272 - P273 - P280 - P302 + P352 - P304 + P340 + P312 RIDADR NONH for all modes of transport Узнать цену -
Plasmin Activity Assay Kit (Fluorometric) MAK244-1KT
NACRES: NA.84 General descriptionPlasmin (EC 3.4.21.7) is a serine protease occurring in plasma as plasminogen. Upon activation via cleavage by plasminogen activators; plasmin solubilizes fibrin clots and activates and/or degrades compounds of the coagulation and complement systems. Plasminogen-Plasmin system has also been implicated in a wide variety of physiologic and pathologic processes, including tumor growth, invasion and metastasis.SuitabilitySuitable to determine the activity of pure plasmin and to detect the activity of plasmin in plasma.PrincipleThe Plasmin Activity Assay Kit is based on the ability of plasmin to proteolytically cleave a synthetic substrate and release a fluorophore, AMC, which can be easily quantified by fluorescence microplate readers. This assay kit is simple, rapid, and can detect plasmin activity as low as 10 ng in a variety of samples.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Plasmin Inhibitor Screening Kit (Fluorometric) MAK245-1KT
NACRES: NA.25 General descriptionPlasmin (EC 3.4.21.7) is a serine protease occurring in plasma as plasminogen. Upon activation via cleavage by plasminogen activators; plasmin solubilizes fibrin clots and activates and/or degrades compounds of the coagulation and complement systems. Plasmin inhibitors are critical in the treatment of hyperfibrinolysis-associated with blood loss and other related complications. A number of biological processes including immune response, fibrinolysis, hemostasis and the elimination of inflammation are affected by serine protease inhibition. Snake venom is considered to be a source of many serine protease inhibitor.SuitabilitySuitable for screening potential inhibitors of plasmin and characterizing/studying plasmin inhibitors in plasma samples.PrincipleThe Plasmin Inhibitor Screening Kit utilizes the ability of plasmin to cleave a synthetic AMC-based peptide substrate and release a fluorophore, AMC, which can be easily quantified by fluorescence microplate readers. In the presence of plasmin specific inhibitors, the extent of cleavage reaction is reduced or completely abolished. The loss in the fluorescence intensity can be correlated to the amount of inhibitor present in the assay solution. The kit provides a simple and rapid method to screen potential inhibitors of Plasmin.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PLTP Activity Assay Kit MAK108-1KT
NACRES: NA.84 General descriptionPhospholipid transfer protein (PLTP) is a protein present in normal human plasma. PLTP transfers phospholipids among lipoproteins in plasma.SuitabilitySuitable to determine Phospholipid transfer protein (PLTP) activity in plasma or serum (fresh or frozen) as well as other sources of PLTP.PrincipleThe PLTP Activity Assay Kit includes proprietary substrates to detect PLTP mediated transfer of fluorescent substrate. Transfer activity results in increased fluorescent emission intensity (λex = 465 nm/λem = 535 nm) from the assay.Analysis NoteLearn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.,Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. 2-8°C Gene Information human ... PLTP(5360)
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Proline Assay Kit (Fluorometric) MAK427-1KT
General descriptionProline is a proteogenic amino acid, which plays an important role in protein folding. In humans, proline can be synthesized from glutamate and arginine. Proline is one of the conditionally essential amino acids in humans. Proline is also produced by honeybees as they process nectar into honey. Thus, its content in honey is often used as an indicator of honey ripeness and sugar adulteration. For mature honey, 180 mg-proline/kg of honey is used as an international standard. Proline contents are measured for its biological importance in plants where it also serves as a stress marker. Unstressed plants contain approximately 0.5 μmol proline per gram of plant tissue while stressed plants can range up to 100 times more proline than the unstressed ones.Features and BenefitsProline Assay Kit:- Compatible with high-throughput handling systems.
- Sensitive, detecting as low as 5 pmol of proline per well.
SuitabilityThe kit is suitable for the measurement of proline in biological samples such as serum, honey and mammalian and plant tissue. The kit can also be used in the analysis of honey maturation and in assaying proline concentration as a stress factor in plants.PrincipleIn this assay, proline is converted to an intermediate, which will further react with a probe to produce a strong fluorometric signal (ex = 535 nm/em = 587nm).ПараметрыQuality Level 100, detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS07,GHS08 signalword Danger hcodes H315 - H319 - H334 - H335 pcodes P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338 Target Organs Respiratory system Узнать цену -
Proteasome 20S Activity Assay Kit MAK172-1KT
EC Number: 200-664-3 NACRES: NA.84 General descriptionProtein degradation occurs most commonly through two major mechanisms, lysosomal degradation and proteasome mediated degradation. Lysosomal degradation occurs through proteolytic enzyme activity and is fairly non-specific. Proteasomal degradation is targeted for a specific protein through the ubiquitination of the target protein. The target protein is typically poly-ubiquitinated and activates the 26S proteasome toward proteolysis. The proteasome 26S is the most common form of the proteasome and is an ATP-dependent proteolytic complex, which contains one 20S (700-kDa) core particle structure and two 19S (700-kDa) regulatory caps.
The proteasome is responsible for the recycling of proteins and maintenance of the balance of protein synthesis and degradation. The proteasome is involved with apoptosis, DNA repair, endocytosis, and cell cycle and has been implicated in disease states such as certain types of cancer, diabetes mellitus, and Alzheimers disease among others making it the target for drug discovery investigations.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for measuring the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates.PrincipleThe fluorometric proteasome 20S assay kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex in cultured cells. This kit uses LLVY-R110 as a fluorogenic indicator for proteasome activities. Cleavage of LLVY-R110 by proteasome generates strongly green fluorescent R110 that is monitored fluorimetrically at 520-530 nm with excitation at 480-500 nm. The assay is robust, and can be readily adapted for high-throughput assays to evaluate the proteasome activities or screen the inhibitors in cultured cells or in solution. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
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Protein Carbonyl Content Assay Kit MAK094-1KT
NACRES: NA.84 General descriptionOxidative stress results when the effectiveness of antioxidant defenses is insufficient to deal with the production of reactive oxygen species (ROS). ROS can induce damage to DNA, lipids, and proteins. The oxidation of proteins results in the production of stable carbonyl groups, which can be used as a measure of oxidative injury.ApplicationProtein carbonyl content assay kit has been used to determine protein carbonyl concentration.SuitabilitySuitable for quantifying carbonyls in protein samplesPrincipleThe Protein Carbonyl Content Assay Kit provides a simple and direct procedure for measuring carbonyl content in a variety of biological samples. Carbonyl content is determined by the derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH) leading to the formation of stable dinitrophenyl (DNP) hydrazone adducts, which can be detected spectrophotometrically at 375 nm, proportional to the carbonyls present.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. 2-8°C
Safety Informationpictograms GHS05,GHS07,GHS09 signalword Danger hcodes H314 - H335 - H410 pcodes P261 - P273 - P280 - P305 + P351 + P338 - P310 - P501 Target Organs Respiratory system RIDADR UN 3316 9 Узнать цену -
Purine Nucleoside Phosphorylase Activity Assay Kit (Colorimetric) MAK289-1KT
NACRES: NA.25 General descriptionPurine nucleoside phosphorylase (PNP) (E.C. 2.4.2.1.) is an enzyme involved in purine metabolism and it catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides, in the presence of inorganic phosphate as a second substrate, to generate the purine base and ribose-1-phosphate or deoxyribose-1-phosphate. It is one of the enzymes of the nucleotide salvage pathways that allow the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). PNP is a cytosolic enzyme.SuitabilitySuitable for the detection of Purine Nucleoside Phosphorylase activity in cell and tissue lysate or purified recombinant protein.PrincipleIn this Purine Nucleoside Phosphorylase Activity Assay, hypoxanthine formed by the breakdown of inosine is further converted to uric acid using a developer. The uric acid is measured at a wavelength of 293 nm. Limit of quantification is 0.1μU recombinant purine nucleoside phosphorylase.Параметрыdetection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05,GHS07,GHS09 signalword Danger hcodes H314 - H317 - H411 pcodes P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 RIDADR UN 3316 9 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric) MAK288-1KT
NACRES: NA.25 General descriptionPurine Nucleoside Phosphorylase (PNP) (E.C. 2.4.2.1.) is an enzyme involved in purine metabolism and it catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides, in the presence of inorganic phosphate as a second substrate, to generate the purine base and ribose-1-phosphate or deoxyribose-1-phosphate. It is one of the enzymes of the nucleotide salvage pathways that allows the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). PNP deficiency leads to severe combined immunodeficiency and also leads to hypouricemia and reduced urinary excretion of uric acid.SuitabilitySuitable for the detection of Purine Nucleoside Phosphorylase activity in cell and tissue lysate or purified recombinant proteinPrincipleIn this Purine Nucleoside Phosphorylase Activity Assay, hypoxanthine formed from the breakdown of inosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the PNP Probe. The fluorescent product is measured at λex = 535nm/λem = 587nm. Limit of quantification is 0.005 μU of recombinant purine nucleoside phosphorylase.Параметрыdetection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS05,GHS08 signalword Danger hcodes H290 - H314 - H334 - H412 pcodes P234 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 RIDADR UN 3316 9 Узнать цену -
Pyridoxal 5-phosphate (Vitamin B6) Fluorometric Assay Kit MAK425-1KT
General descriptionPyridoxal 5-phosphate (PLP), the active form of Vitamin B6 is a cofactor in a variety of metabolic reactions including transamination, decarboxylation, racemization, etc. Vitamin B6 comprises of pyridoxine, pyridoxamine and pyridoxal, which during metabolism is converted to the enzymatically active form namely PLP. Vitamin B6 is necessary for the synthesis of neurotransmitters, hemoglobin in red blood cells, fat metabolism etc. Vitamin B6 deficiency can lead to nervous disorders, peripheral neuropathy, anemia, seizures etc.Features and BenefitsPyridoxal 5-phosphate (Vitamin B6) Assay Kit:- Compatible with high-throughput handling systems.
- Detects as low as 0.2 pmol/well of PLP in biological samples.
SuitabilityThe kit is suitable for the measurement of PLP in biological samples (ex. serum and mammalian tissue) and beverages and for the analysis of Vitamin B6 intake on serum.PrincipleIn this assay, PLP reacts with a PLP-dependent enzyme thereby converting the substrate into an intermediate, which will further react with a probe to produce a strong fluorometric signal (ex = 535 nm/em = 587 nm).ПараметрыQuality Level 100, detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS05,GHS08 signalword Danger hcodes H315 - H317 - H318 - H334 - H373 pcodes P260 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338 Target Organs Respiratory system Узнать цену -
Pyrophosphate (PPi) Assay Kit (Fluorometric/Colorimetric) MAK386-1KT
General descriptionInorganic pyrophosphate (diphosphate, pyrophosphoric acid or PPi) is a small molecule formed during, or required as a substrate, for a number of biochemical reactions. Enzymatic reactions that generate PPi include ATP hydrolysis, DNA and RNA polymerization, cyclic AMP formation, and enzymatic activation of fatty acids to form their CoA esters. Conversely, enzymes that utilize PPi may include cyclases, hydrolases and ligases. Measurement of inorganic pyrophosphate (PPi) in plasma, serum and other biologic fluids is important in studies of bone metabolism, renal stone disease, and certain types of arthritis.Features and BenefitsThe Pyrophosphate (PPi) Assay Kit is:- Compatible with high-throughput handling systems.
- Specific for Pyrophosphate: other molecules containing phosphate groups do not interfere with the assay.
- Detects as little as 2 µM of Pyrophosphate in a variety of biological samples.
SuitabilityThe kit is suitable for the determination of PPi concentration, monitoring pyrophosphate release by a variety of enzymes and for the screening inhibition or activities of enzymes that consume pyrophosphate. Suitable samples include plasma, serum and other biological fluids, cell culture extracts and tissue lysates.PrincipleThe Pyrophosphate (PPi) Assay Kit provides a fast, convenient and ultrasensitive method for determination of free inorganic pyrophosphate levels in biological material. PPi produced during biotic processes is detected through a series of reactions which utilize a proprietary enzyme mix and probe, generating a stable product that can be quantified by either colorimetric or fluorometric readout. Generated fluorescence (ex = 535 nm/em = 587nm) or color (A570 nm) intensities are directly proportional to the concentrations of pyrophosphate, enabling precise measurements. Monomeric inorganic phosphate (Pi) does not interfere with the assay.ПараметрыQuality Level 100, detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 pcodes P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 Узнать цену -
Pyrophosphate Assay Kit MAK168-1KT
EC Number: 200-664-3 NACRES: NA.84 General descriptionPyrophosphate (PPi) is produced by a number of biochemical reactions such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters. It is hydrolyzed by inorganic pyrophosphatase. PPi is present in the blood and in the extracellular matrix of tissues.SuitabilityThe Pyrophosphate Assay Kit provides a simple and direct microplate assay procedure for measuring pyrophosphate in a variety of samples.PrincipleThe pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (ex =316/em = 456 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.Параметрыusage sufficient for 200 fluorometric tests (Blue fluorescence) detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS05 signalword Danger hcodes H318 pcodes P280 - P305 + P351 + P338 RIDADR NONH for all modes of transport Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Pyruvate Assay Kit MAK332-1KT
General descriptionPyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for pyruvate detection in biological samples and for studying the effects of drugs on pyruvate metabolism.PrincipleThe Pyruvate Assay Kit uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λem/ex = 585/530nm is directly proportional to pyruvate concentration in the sample. Linear detection range in 96-well plate: 2 to 500 μM (17 μg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 μM for fluorometric assays.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric
Safety InformationWGK Germany WGK 2 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Pyruvate Assay Kit MAK071-1KT
NACRES: NA.84 General descriptionPyruvate is a central molecule in metabolism that is used and synthesized through multiple metabolic pathways including glycolysis and the transamination of alanine. Pyruvate may be further oxidized in the citric acid cycle, converted to carbohydrates during gluconeogenesis, or reduced to lactate. High levels of pyruvate are associated with liver disease and genetic disorders. Pyruvate has also been used to stimulate metabolism leading to loss of body weight.
SuitabilitySuitable for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells.PrinciplePyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (ex = 535/em = 587 nm) product, proportional to the pyruvate present.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. 20°C Gene Information human ... PKLR(5313), PKM2(5315)
mouse ... PKLR(18770), PKM2(18746)
rat ... PKLR(24651), PKM2(25630)
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 - H412 pcodes P261 - P272 - P273 - P280 - P284 - P302 + P352 RIDADR NA 1993 / PGIII Узнать цену -
Pyruvate Kinase Activity Assay Kit MAK072-1KT
NACRES: NA.84 General descriptionPyruvate kinase (PK) is an enzyme that catalyzes the final step of glycolysis, the transfer of a phosphate group from phospho(enol)pyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase deficiency, due to defects in pyruvate kinase expression or activity, is the second most common cause of hemolytic anemia.
The Pyruvate Kinase Activity Assay Kit provides a simple and direct procedure for measuring pyruvate kinase activity in a variety of biological samples. Pyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (ex = 535/em = 587 nm) product, proportional to the pyruvate present. One unit of pyruvate kinase is the amount of enzyme that will transfer a phosphate group from PEP to ADP to generate 1.0 µmole of pyruvate per minute at 25 °C.ApplicationPyruvate Kinase Activity Assay Kit has been used to determine the activity of pyruvate kinase in samples.SuitabilitySuitable for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etcPrincipleThe Pyruvate Kinase Activity Assay Kit provides a simple and direct procedure for measuring pyruvate kinase activity in a variety of biological samples. Pyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate present. One unit of pyruvate kinase is the amount of enzyme that will transfer a phosphate group from PEP to ADP to generate 1.0 μmole of pyruvate per minute at 25 °C.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 - H412 pcodes P261 - P272 - P273 - P280 - P284 - P302 + P352 RIDADR NA 1993 / PGIII Узнать цену -
Reduced Glutathione (GSH) Assay Kit (Colorimetric) MAK364-1KT
General descriptionGlutathione (GSH), a thiol-containing tripeptide (γ-glutamyl-cysteinyl-glycine), is a key antioxidant in many species. It has been highly implicated in the detoxification/elimination of xenobiotics (naturally occurring harmful compounds such as free radicals, hydroperoxides etc.) and in the maintenance of the oxidation state of protein sulfhydryl groups. In addition, GSH plays a pivotal role in the pathogenesis of numerous human diseases including cancer and cardio-vascular diseases. Glutathione is present in cells in both reduced (GSH) and oxidized (GSSG) forms- GSH being, the predominant species under normal physiological conditions inside cells. Thus, pathologic conditions causing oxidative stress would result in increased levels of GSSG. Therefore, the measurement of intracellular GSH appears to be a sensitive indicator of the overall cell health, and its ability of resisting toxic challenges.SuitabilityThe kit is suitable for the measurement of reduced glutathione in various biological samples/preparations including tissue homogenates (liver, etc.) and cell lysates (Hep G2, Jurkat, etc.).PrincipleThe Reduced Glutathione (GSH) Assay Kit is based on an enzymatic cycling method in the presence of GSH and a chromophore. The reduction of the chromophore produces a stable product, which can be followed kinetically at A450 nm. Therefore, its absorbance is directly proportional to the amount of GSH in the sample. The kit is highly specific and sensitive because GSSG does not interfere with the assay. The kit includes 5-Sulfosalicylic acid (SSA) in order to protect samples GSH endogenous content. (SSA acts as a deproteinizing and antioxidant agent. The assay is simple, reproducible and can specifically detect as low as 50 pmol/well of reduced form of Glutathione (GSH) in a 100 μL reaction.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05,GHS08 signalword Danger hcodes H314 - H317 - H334 - H341 pcodes P202 - P261 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Renin Assay Kit MAK157-1KT
NACRES: NA.84 General descriptionRenin, also known as angiotensinogenase, is an aspartyl protease that cleaves angiotensinogen to form angiotensin I, the initial and rate-limiting step of the renin-angiotensin system (RAS). This system plays a critical role in the regulation of blood pressure and fluid balance. Increased renin activity can result in hypertension due to increased vasoconstriction and fluid retention. Inhibitors of renin can be used as a treatment for hypertension.ApplicationRenin Assay Kit has been used to determine the activity of renin.SuitabilityThe Renin Assay Kit provides a convenient and simple method for screening renin activity in a variety of samples or for screening renin inhibitors.PrincipleRenin activity is determined using a proprietary fluorescence resonance energy transfer (FRET) peptide containing a fluorescence quencher. Cleavage of the peptide by renin results in the production of a fluorescent product (ex = 540/em = 590 nm). This assay is ~50-fold more sensitive than the EDANS/DABCYL-based assay.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. 20°C Gene Information human ... REN(5972)
rat ... REN(24715)
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Salicylate Assay Kit (Colorimetric) MAK415-1KT
General descriptionSalicylates (salts of 2-hydroxybenzoic acid) are common non-steroidal anti-inflammatory drugs (NSAIDs) with anti-pyretic and mild analgesic effects. Originally discovered in willow tree bark, salicylate has been used in an ethnopharmacological context for millennia to treat inflammation and fever. The most frequently used modern salicylate drug is aspirin (acetylsalicylic acid), which is rapidly hydrolyzed to salicylate in both the gastrointestinal tract and bloodstream. Salicylate acts as a weak inhibitor of the proinflammatory COX-2 enzyme, which is thought to underlie its anti-inflammatory effect in chronic aspirin therapy. However, supratherapeutic blood salicylate levels can cause severe intoxication and poisoning. Serum salicylate levels are monitored in people taking chronic high-dose aspirin for arthritis and in suspected cases of aspirin overdose. Therapeutic serum levels for salicylate range from 50-250 mg/mL (0.36 – 1.8 mM) in individuals on chronic aspirin therapy. Levels over 300 mg/ml (2.17 mM) are considered toxic, resulting in dose-dependent and potentially lethal symptoms such as tinnitus/deafness, lethargy/coma, seizures and metabolic acidosis.Features and BenefitsSalicylate Assay Kit:- Compatible with high-throughput handling systems.
SuitabilityThe kit is suitable for the estimation of salicylate concentration in blood (plasma or serum).PrincipleIn the assay, salicylate is enzymatically metabolized to catechol, with concomitant oxidation of a cofactor, resulting in a decrease in absorbance at A405 nm that is proportional to the concentration of salicylate present. The assay is not affected by other NSAIDs and has an LOQ of 0.19 mM with a reliable linear range from 1 – 20 nmole salicylate per well (corresponding to 0.2 – 4 mM salicylate in undiluted samples).ПараметрыQuality Level 100, detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05,GHS07,GHS08 signalword Danger hcodes H302 - H318 - H361d pcodes P201 - P202 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313 Узнать цену
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