Metabolism Assay Kits
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Fatty Acid Methyl Ester Purification Kit MAK225-1KT
General descriptionFatty Acid Methyl Ester Purification Kit is used to further purify samples prepared with the Fatty Acid Methylation Kit (MAK224). Fatty acid samples are required to be cleaned with this kit before the analysis by a capillary column.SuitabilitySuitable for the purification of methylated fatty acid samples such as blood, yeast, liver, cooking oil, soybean powder, fish oils, etc.PrincipleThe Fatty Acid Methylation Kit (Catalog Number MAK224) followed by the Fatty Acid Methyl Ester Purification Kit employs a proprietary reaction technique. Using these kits, the fatty acid methyl esterification/purification process is greatly simplified.Параметрыusage sufficient for 50 tests storage temp. room temp
Safety Informationpictograms GHS02 signalword Danger hcodes H225 pcodes P210 - P233 - P240 - P241 - P242 - P243 RIDADR UN 3316 9 Flash Point F -14.8 °F - closed cup Flash Point C -26.0 °C - closed cup Узнать цену -
Fatty Acid Methylation Kit MAK224-1KT
NACRES: NA.25 General descriptionMethyl esterification of fatty acids is commonly done prior to gas chromatography analysis to prevent peak tailing and to increase sample volatility. However, the conventional esterification procedure requires specialized equipment and high technical skill. By using the Fatty Acid Methylation kit that employs a new reaction technique, followed by the fatty acid methyl ester purification kit (MAK225), fatty acid methyl esterification is greatly simplified.SuitabilitySuitable for the methyl esterification of fatty acids (glycerolipids such as triglycerides, phospholipids, glycolipids, lecithin and sterol esters). Samples include blood, yeast, liver, cooking oil, soybean powder, fish oil, etc.PrincipleThe Fatty Acid Methylation Kit followed by the Fatty Acid Methyl Ester Purification Kit (Catalog Number MAK225) employs a proprietary reaction technique. Using these kits, the fatty acid methyl esterification/purification process is greatly simplified.Параметрыusage sufficient for 100 tests storage temp. room temp
Safety Informationpictograms GHS02,GHS05,GHS06,GHS08,GHS09 signalword Danger hcodes H225 - H251 - H290 - H301 + H311 + H331 - H304 - H314 - H336 - H361fd - H370 - H373 - H373 - H411 pcodes P210 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Target Organs Central nervous system, Eyes, Nervous system Supp Hazards EUH014 RIDADR UN 3316 9 WGK Germany WGK 3 Flash Point F -14.8 °F Flash Point C -26 °C Узнать цену -
Fatty Acid Uptake Kit MAK156-1KT
EC Number: 200-664-3 General descriptionFatty acids are critical for many biological processes including energy metabolism and the synthesis of structural cellular components. The facilitated uptake of fatty acids, presumably through fatty acid transport proteins, is thought to be regulated at multiple levels. The dysregulation of fatty acid uptake may contribute to diseases such as obesity, type 2 diabetes, and hepatic steatosis.SuitabilityThis kit is suitable for the measurement of fatty acid uptake in cells containing fatty acid transportersPrincipleThis kit is provides a simple and sensitive method for the measurement of fatty acid uptake in cells containing fatty acid transporters. This kit uses a proprietary dodecanoic acid fluorescent fatty acid substrate whose uptake into cells result in an increase in fluorescence intensity (λex = 485/λem = 515 nm).Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric) MAK369-1KT
General descriptionAntioxidants are molecules which act as reducing agents by donating electrons to free radicals to stabilize them and minimize the damage caused by free radicals to DNA, cells and organ systems. Antioxidants include substances such as polyphenols; flavonoids; vitamins and enzymes like glutathione peroxidase and superoxide dismutase. They are known to have beneficial health effects such as lowering the risk of cancer, heart disease and neurodegenerative disorders and are abundantly found in plants, fruits, vegetables, beverages and natural products.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the measurement of antioxidant capacity in fruits, vegetables, beverages (like tea and wine), food products, plant extracts, herbal products, serum and plasma.PrincipleFerric reducing antioxidant power (FRAP) assay is a widely used method that uses antioxidants as reductants in a redox-linked colorimetric reaction, wherein Fe3+ is reduced to Fe2+. Ferric (Fe3+) to ferrous (Fe2+) ion reduction at low pH causes formation of a colored ferrous-probe complex from a colorless ferric-probe complex. The assay is high-throughput adaptable and can detect antioxidant capacities as low as 0.2 mM Fe2+ equivalents.Параметрыusage sufficient for 200 colorimetric tests detection method colorimetric
Safety Informationpictograms GHS05 signalword Warning hcodes H290 pcodes P234 - P390 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Fluorimetric Hydrogen Peroxide Assay Kit MAK166-1KT
EC Number: 231-765-0 NACRES: NA.84 General descriptionHydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.ApplicationFluorimetric Hydrogen Peroxide Assay Kit has been sued to determine the oxidation pressure (H2O2).Features and BenefitsThis assay is compatible with high-throughput handling systems.SuitabilityThe Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.PrincipleFluorimetric Hydrogen Peroxide Assay Kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates an infrared fluorescent product (ex = 640/em = 680 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader. The generation of the fluorescence product is pH independent between pH 4 to pH 10 and is superior to other fluorescence kits for measuring hydrogen peroxide in low pH solutions which often display decreased probe sensitivity. The infrared fluorescent probe is less sensitive to assay background due to autofluorescence from biological samples. This assay is compatible with high-throughput handling systems.Параметрыusage sufficient for 500 fluorometric tests (infrared fluorescence) detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS03,GHS05,GHS07 signalword Danger hcodes H271 - H302 + H332 - H314 pcodes P220 - P280 - P305 + P351 + P338 - P310 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorimetric Hydrogen Peroxide Assay Kit MAK165-1KT
General descriptionHydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.Features and BenefitsCompatible with high-throughput handling systems.SuitabilityThis kit is suitable to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.PrincipleThe Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions. This kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates a red fluorescent product (ex = 540/em = 590 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader.Параметрыusage sufficient for 500 fluorometric tests (red fluorescence) detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H302 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorometric Aldehyde Assay Kit MAK141-1KT
EC Number: 200-664-3 NACRES: NA.84 General descriptionAldehydes are highly reactive molecules, which play a role in many physiological, pathological and commercial processes. They have been implicated as environmental pollutants, and in neurodegeneration and DNA damage. A method for rapid and accurate measurement of aldehydes is an important tool for biological and chemical research, the food industry, and environmental pollution surveillance.
The Fluorometric Aldehyde Assay Kit provides a simple and direct procedure for measuring aldehydes in samples in which the pH is physiological and higher. Aldehyde reacts with a fluorogenic dye, resulting in a fluorometric (ex = 365/em = 435 nm) product proportional to the amount of aldehydes present. The Fluorometric Aldehyde Assay Kit has a lower limit of detection of 0.3 nanomole of aldehyde in a 100 µL assay volume (3 µM).
Aldehydes are highly reactive molecules, which play a role in many physiological, pathological and commercial processes. They have been implicated as environmental pollutants and in neurodegeneration and DNA damage. A method for rapid and accurate measurement of aldehydes is an important tool for biological and chemical research, the food industry and environmental pollution surveillance.SuitabilityThis kit is suitable for the detection of aldehydes in a variety of samples, particularly when the samples are at a higher pH. When working with samples in a low pH, product number MAK140, Colorimetric Aldehyde Assay, is more suitable.PrincipleThe fluorometric aldehyde assay kit provides a simple and direct procedure for measuring aldehydes in samples in which the pH is physiological and higher. Aldehyde reacts with a fluorogenic dye, resulting in a fluorometric (ex = 365/em = 435 nm) product proportional to the amount of aldehydes present. The fluorometric aldehyde assay has a lower limit of detection of 0.3nanomole of aldehyde in a 100µL assay volume (3µM).Параметрыusage sufficient for 200 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS05 signalword Warning hcodes H290 pcodes P234 - P390 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorometric Intracellular pH Assay Kit MAK150-1KT
General descriptionIntracellular pH changes are implicated in diverse physiological and pathological processes, including cell proliferation, apoptosis, fertilization, malignancy, multidrug resistance, ion transport, lysosomal storage disorders, and Alzheimers disease.
This kit can be utilized to measure decreases in intracellular pH in cells treated under various conditions using a simple standard protocol. Alternatively, an Acid-Load procedure can be used to measure the increase of intracellular pH associated with changes in cellular metabolism due to GPCR activation, growth factor activity, or other cellular stimulation. With the Acid-Load procedure ammonium chloride solution is added after the fluorescent pH dye is loaded into cells in a minimum volume. This Acid-Loading step is followed by the addition of agonist in a relatively large volume (~4x) of buffer. The sudden volume change initiates an efflux of ammonia (NH3) from the cells causing a rapid decrease in intracellular pH, and thus a decrease in fluorescence signal. The effect of agonist on the subsequent recovery of intracellular pH is measured by the relative fluorescence signal increase.ApplicationFluorometric Intracellular pH Assay Kit has been used to measure the intracellular pH.Features and BenefitsSuperior alternative to BCECFSuitabilityThis kit can be utilized to measure changes in intracellular pH in cells treated under various conditions.PrincipleThe Fluorometric Intracellular pH Assay Kit utilizes a proprietary cell-permeable fluorescent indicator, BCFL-AM, for measuring relative intracellular pH changes. BCFL-AM is a superior alternative to the popular ratiometric pH probe, 2,7-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). Unlike BCECF, which is a complex and variable mixture of isomers, BCFL-AM is a single isomer species. BCFL-AM has the same spectral responses as BCECF-AM (ex = 490 nm/em = 535 nm for single measurement or ex = 505 nm/em = 535 nm and ex = 430 nm/em = 535 nm for ratio measurement) but enhanced reproducibility. BCFL-AM exhibits pH-dependent dual excitability, pKa ~7.0, and an excitation isosbestic point of 454 nm.Параметрыusage sufficient for 1000 fluorometric tests detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorometric Intracellular Ros Kit MAK145-1KT
NACRES: NA.25 General descriptionReactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.ApplicationFluorometric intracellular reactive oxygen species (ROS) Kit has been used for detection of intracellular ROS in live cells.SuitabilityThis kit is suitable for the detection of intracellular ROS by fluorescence microplate reader or a fluorescent microscope.PrincipleThe kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (ex = 520/em = 605 nm) proportional to the amount of ROS present The assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader.Параметрыusage sufficient for 200 fluorometric tests (red) detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorometric Intracellular Ros Kit MAK142-1KT
EC Number: 200-664-3 NACRES: NA.25 General descriptionReactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.SuitabilityThis kit is suitable for the detection of intracellular ROS by fluorescence microplate reader, flow cytometer, or a fluorescent microscope with TRITC filter.PrincipleThe Fluorometric Intracellular ROS Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells after 1 hour incubation. ROS react with a fluorogenic sensor localized to the cytoplasm, resulting in a fluorometric product proportional to the amount of ROS present. The assay can be performed in either a 96 or 384 multiwell plate format and with detection at λex = 650/λem = 675 nm.Параметрыusage sufficient for 200 fluorometric tests (Deep red) detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Fluorometric Intracellular Ros Kit MAK144-1KT
EC Number: 200-664-3 NACRES: NA.25 General descriptionReactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation above the biological system′s detoxification capacity results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.ApplicationFluorometric intracellular reactive oxygen species (ROS) Kit has been used to evaluate the mitochondrial damage by mechanical ventilation (MV). It is also used to measure the production of intracellular ROS.SuitabilityThis kit is suitable for the detection of intracellular ROS by fluorescence microplate reader, flow cytometer, or a fluorescent microscope with TRITC filter.PrincipleThe Fluorometric Intracellular ROS Assay Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (ex = 540/em = 570 nm) proportional to the amount of ROS present. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.Параметрыusage sufficient for 200 fluorometric tests (orange) detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorometric Intracellular Ros Kit MAK143-1KT
NACRES: NA.25 General descriptionReactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.ApplicationFluorometric Intracellular Ros Kit has been used in reactive oxygen species (ROS) generation assay.SuitabilityThis kit is suitable for the detection of intracellular ROS by fluorescence microplate reader or a fluorescent microscope.PrincipleThe Fluorometric Intracellular ROS Assay Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells within 1 hour incubation. ROS react with a fluorogenic sensor localized to the cytoplasm, resulting in a fluorometric product (ex = 490/em = 520 nm) proportional to the amount of ROS present. The assay can be performed in either a 96 or 384 multiwell plate format and read using a fluorescence microscope or a fluorescence microplate reader.
Параметрыusage sufficient for 200 fluorometric tests (green) detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorometric Maleimide Assay Kit MAK167-1KT
EC Number: 204-892-4 NACRES: NA.84 General descriptionMaleimide is an unsaturated imide used in protein conjugation reactions.SuitabilityThe assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader.PrincipleThe Fluorometric Maleimide Assay Kit provides a sensitive, one-step fluorometric assay to detect maleimide, which reacts with a proprietary dye, resulting in a fluorometric product (λex = 490/λem = 520 nm) proportional to the amount of maleimide present. The assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader.Параметрыusage sufficient for 200 fluorometric tests detection method fluorometric storage temp. −20°C
Safety InformationRIDADR UN 3316 9 WGK Germany WGK 3 Узнать цену -
Fluorometric Sphingomyelin Assay Kit MAK154-1KT
NACRES: NA.84 General descriptionSphinogmyelin, a sphingolipid consisting of a phospholipid (typically phosphocholine) attached to a ceramide moiety, is a major component of cellular membranes. Sphingomylein is enriched in the exoplasmic leaflet of the cell membrane. Within the cell membrane, sphingomyelin interacts with cholesterol to form lipid rafts. The metabolism of sphingomyelin contributes to cellular signaling via the release of ceramide, a second messenger that participates in multiple cellular signaling pathways. Sphingomyelin accumulates in Niemann-Pick disease due to deficiencies in sphingomyelinase activity.SuitabilityThis kit is suitable for the detection of sphingomyelin in a variety of samples such as blood and cell extracts.PrincipleSphingomyelin is determined by a coupled enzyme assay, in which the hydrolysis of sphingomyelin by sphingomyelinase produces phosphocholine, which results in a fluorescent product (ex =540/em = 590 nm) proportional to the Sphingomyelin present.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS05,GHS08,GHS09 signalword Danger hcodes H317 - H318 - H334 - H410 pcodes P261 - P273 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
Fluorometric Sphingomyelinase Assay Kit MAK155-1KT
NACRES: NA.84 General descriptionSphingomyelinase (SMase), or sphingomyelin phosphodiesterase, catalyzes the hydrolytic cleavage of sphingolipid to phosphocholine and ceramide, a second messenger involved in multiple cellular responses including the cellular response to stress. There are at least five types of SMases identified, which are classified according to their cation dependence, cellular location, and pH optima for activity, and include the acidic and neutral sphingomyelinase. Neutral SMases, along with the acidic SMases, are thought to be major contributors to the production of ceramide in response to cellular stress. Mutations in is associated with metabolic disorders like Niemann-Pick disease B.SuitabilityThis kit is suitable for the detection of sphingomyelin in a variety of samples such as blood and cell extracts.PrincipleThe Sphingomyelinase Assay Kit provides a simple and direct procedure for measuring neutral SMase activity in a variety of samples such as blood or cell extracts, or for screening SMase activators and inhibitors. SMase activity is determined by a coupled enzyme assay, which results in a fluorescent product (λex =540/λem = 590 nm) proportional to the SMase activity present.Параметрыusage sufficient for 200 fluorometric tests (red fluorescence) detection method fluorometric storage temp. 20°C Gene Information human ... SMPD2(6610), SMPD3(55512), SMPD4(55627)
mouse ... SMPD2(20598), SMPD3(58994), SMPD4(77626)
rat ... SMPD2(83537), SMPD3(94338), SMPD4(303790)
Safety Informationpictograms GHS05,GHS08,GHS09 signalword Danger hcodes H317 - H318 - H334 - H410 pcodes P261 - P273 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
Fluorometric Thiol Assay Kit MAK151-1KT
EC Number: 200-664-3 NACRES: NA.84 General descriptionThiol groups, found as free cysteine, glutathione (GSH), and cysteine residues in proteins, are involved in many biological processes. The disulfide bonds generated when the thiol groups of two cysteine residues are oxidized contribute to the tertiary or quatenary structure of a protein.ApplicationFluorometric Thiol Assay Kit has been used to determine the lipid peroxidation expressed as thiols in samples.SuitabilityThis kit is suitable for the detection of thiols in a variety of different biological samples including plasma, urine, and cell extracts. This kit is not optimized for measuring thiol groups in proteins.PrincipleThe Fluorometric Thiol Quantitation Kit provides an ultrasensitive assay to quantitate the thiol content of small molecules. The proprietary thiol sensor generates a strongly fluorescent adduct (λex = 490/λem = 520 nm) upon reacting with a thiol-containing compound. In addition, both absorption and emission spectra of the thiol adduct are pH-independent, making this assay kit highly robust. The kit can detect as little as 1 picomole of cysteine or GSH in a 100 μL assay volume (10 nM). The assay can be performed conveniently in either a 96 or 384 multiwell plate format.Параметрыusage sufficient for 200 fluorometric tests detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Formaldehyde Assay MAK131-1KT
NACRES: NA.84 General descriptionFormaldehyde is widely employed in industry for a wide range of applications. It acts as a disinfectant and biocide, and is utilized as a tissue fixative and embalming agent. Formaldehyde is a key metabolite produced during normal metabolism and contributes to methylation reactions and protein and nucleic acid synthesis. Increased production of formaldehyde has been observed in many cancer cell lines and increased formaldehyde concentration in urine has been associated with bladder and prostrate cancer.Features and BenefitsCompatible with high-throughput handling systems. Can be adapted for use with cuvettes.SuitabilitySuitable for the detection of formaldehyde in urine and other biological samples.PrincipleThe Formaldehyde Assay kit provides a simple and direct procedure for measuring formaldehyde levels in urine and other biological samples. In this assay, formaldehyde is derivatized with acetoacetanilide in the presence of ammonia resulting in a fluorescent (λex = 370/λem = 470 nm) product, proportional to the formaldehyde present.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. 2-8°C
Safety Informationpictograms GHS05,GHS07,GHS08,GHS09 signalword Danger hcodes H290 - H302 - H314 - H335 - H370 - H410 pcodes P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Target Organs Respiratory system RIDADR UN 3316 9 WGK Germany WGK 3 Узнать цену -
Formate Assay Kit
NACRES: NA.84
Кат. номер MAK059-1KT MAK059-1KT-CUS General descriptionFormate, the salt form of formic acid, is the simplest carboxylate anion. Formate is produced in the mitochondria as a result of the oxidation of one-carbon donors such as serine. Mitochondrially-derived formate is utilized for cytoplasmic one-carbon metabolism in the de novo synthesis of purines, thymdiylate, and the remethylation of homocysteine to methionine. Under normal physiologic conditions, formate is present at low levels in serum and blood but levels may be increased following acute methanol poisoning or environmental exposure to formaldehyde. Serum and urine formate levels were increased in vitamin B12-deficient rats and may be indicative of hyperhomocysteinemia due to defects in remethylation.ApplicationFormate Assay kit has been used to determine the concentration of formate in samples.SuitabilitySuitable for serum, urine, media, cells, and tissues.PrincipleThe Formate Assay Kit provides a simple, sensitive, and rapid means of quantifying formate in a variety of samples including serum, urine, media, cells, and tissues. Formate concentrations are determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the formate present.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05 signalword Danger hcodes H314 pcodes P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405 RIDADR NONH for all modes of transport Узнать цену -
Free Fatty Acid Quantitation Kit MAK044-1KT
General descriptionFree fatty acids, also know as nonesterified fatty acids, circulate in the plasma bound to albumin. The majority of free fatty acids are derived from either dietary sources or are mobilized from adipose tissue. Plasma free fatty acids are elevated in many obesity-related disorders and may contribute to insulin resistance in peripheral tissues. Conditions such as sepsis and tumors producing lipoactive homones may also be associated with elevated free fatty acid levels.
Abnormalities in fatty acid metabolism causes fatty liver disease. Accumulation of free fatty acid in myocytes leads to the risk of cardiac dysfunction. Increased fatty acid -oxidation is implicated in cardiac ischemia.ApplicationFree Fatty Acid Quantitation Kit has been used in the following:- to measure lipid hydrolysis, by determining the free fatty acid in digested sample.
- to measure plasma and serum free fatty acids level.
- to quantify free fatty acids level in liver tissue.
SuitabilitySuitable for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc.PrincipleIn this kit, the concentration of fatty acids (C8 and longer) is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (ex = 535/em = 587 nm) product, proportional to the fatty acids present.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety InformationPictograms GHS07,GHS09 Signal Word Warning Hazard Statements H319 - H411 Precautionary Statements P264 - P273 - P280 - P337 + P313 - P391 - P501 RIDADR NA 1993 / PGIII WGK Germany WGK 3 Flash Point(F) 188.6 °F - closed cup Flash Point(C) 87 °C - closed cup Узнать цену -
Free Glycerol Determination Kit FG0100-1KT
NACRES: NA.26 General descriptionGlycerol, also referred to as glycerin or glycerine, is a 3 carbon sugar alcohol that forms the backbone of fatty acids. It is a central component for synthesis of all lipids, and acts as a backbone for triglycerides and phospholipids, which play an important role in cell membrane structure. Due to its low toxicity, glycerol is widely used in pharmaceutical, food and cosmetic formulations and is the main waste by-product of biodiesel production via transesterification. In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.ApplicationThe triglyceride and free glycerol kits are for the quantitative determination of glycerol, total triglycerides or free triglycerides.
The Free Glycerol Determination Kit has been used to determine the level of glycerol released into the medium from mammalian cells grown in vitro .
The Free Glycerol Determination Kit measures free, endogenous glycerol using coupled enzyme reactions and does not include initial lipase hydrolysis. Glycerol is phosphorylated by adenosine-5-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H2O2). Peroxidase catalyzes the coupling of H2O2 with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to the free glycerol concentration of the sample.SuitabilitySuitable for the quantitative enzymatic determination of glycerol in serum or plasma.LinkageIn addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.ПараметрыQuality Level 200, usage 1 kit sufficient for 1000 reactions detection method colorimetric storage temp. 2-8°C
Safety Informationpictograms GHS05,GHS07,GHS09 signalword Danger hcodes H302 - H315 - H318 - H410 pcodes P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 RIDADR UN 3077 9 / PGIII Узнать цену -
Fructose Colorimetric/Fluorometric Assay Kit MAK265-1KT
NACRES: NA.84 General descriptionFructose is a monosaccharide naturally found in honey and fruits. Several studies have linked fructose to the development of the metabolic syndrome particularly through promoting insulin resistance in the liver. It has also been implicated in the obesity epidemic. Studies in animals have revealed high fructose intake during pregnancy and lactation may lead to metabolic dysfunctions in the mother and the newborn. Fructose measurements can provide useful insights into metabolic and biochemical functions. Excessive uptake of fructose is implicated in obesity in children. Fructose induced hyperuricemia, reduces nitric oxide bioavailability for insulin and thus it is implicated in diabetes. Fructose might be implicated in cardiovascular disease, as it is the only sugar that raises uric acid production in the body. High fructose diet is known to induce hypertension.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for detecting fructose in cell and tissue culture supernatants. Note: Not suitable for urine, plasma, or serum.PrincipleIn this assay kit, fructose is converted to -glucose resulting in a colorimetric (570 nm)/fluorometric (ex = 535 nm/em = 587 nm) product proportional to fructose present. This kit has a linear range of detection between 0.2–1.0 nmole fructose for the fluorometric assay and 2–10 nmole fructose for the colorimetric assay.Параметрыdetection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 - H412 pcodes P261 - P272 - P273 - P280 - P284 - P302 + P352 RIDADR UN 3316 9 WGK Germany WGK 3 Узнать цену -
Fructose-6-Phosphate Assay Kit MAK020-1KT
NACRES: NA.84 General descriptionFructose-6-phosphate (F6P) is a glycolytic pathway intermediate produced by the isomerization of glucose-6-phosphate by phosphoglucose isomerase. F6P is further phosphorylated to fructose 1,6-biphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. F6P can also be shunted from glycolysis to the non-oxidative branch of the pentose phosphate pathway as a means of generative pentose phosphates for nucleotide synthesis. F6P levels are elevated in rapidly proliferating cells such as cancer cells. The Fructose-6-phosphate Assay Kit is a highly sensitive and simple fluorescence-based method of quantifying F6P in a variety of samples.ApplicationFructose-6-Phosphate Assay Kit has been used to measure fructose-6-phosphate (F-6-P) and glucose-6-phosphate (G6P).SuitabilitySuitable for quantifying Fructose-6-Phosphate in a variety of samplesPrincipleFructose-6-phosphate concentration is determined by a coupled enzyme reaction, which results in a fluorometric (ex = 535 nm/em = 587 nm) product, proportional to the fructose-6-phosphate present. Typical sensitivities for this kit are 0.01 to 0.5 nmoles of F6P.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NA 1993 / PGIII Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Fumarase Activity Colorimetric Assay Kit MAK206-1KT
NACRES: NA.84 General descriptionFumarase (Fumarate Hydratase; EC 4.2.1.2) catalyzes the reversible hydration of fumarate to malate in the tricarboxylic acid (Krebs) cycle. The activity of fumarase is important as it produces reducing equivalents that drive the synthesis of ATP and maintains energy balance in aerobic metabolism. A deficiency of fumarase activity results in progressive encephalopathy.ApplicationFumarase Activity Colorimetric Assay Kit has been used to determine fumarase (FUM) activity.SuitabilitySuitable for the detection of Fumarase in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.PrincipleThe Fumarase Activity Colorimetric Assay Kit provides a simple and high-throughput ready procedure for measuring fumarase activity in a variety of tissues, cells, and isolated mitochondria. Fumarase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of fumarase is the amount of enzyme that generates 1.0 mmole of NADH per minute at pH 9.5 at 37 °C.Параметрыdetection method colorimetric storage temp. 20°C Gene Information human ... FH(2271)
mouse ... FH(14193)
rat ... FH(24368)
Safety Informationpictograms GHS05 signalword Danger hcodes H314 pcodes P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405 RIDADR NONH for all modes of transport Узнать цену -
Fumarate Assay Kit MAK060-1KT
General descriptionFumarate is a tricarboxylic acid (TCA) cycle intermediate formed by the oxidation of succinate by succinate dehydrogenase. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. Deficiencies in fumarate hydratase are associated with increased cellular concentrations of fumarate and increased incidences of renal cell cancer. This is most likely due to fumarate-mediated upregulation of HIF1. The Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples.ApplicationFumarate Assay Kit has been used to determine the concentration of fumarate in samples.SuitabilitySuitable for the detection of fumarate in a variety of samples including tissue, cells and serumPrincipleFumarate is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional to the fumarate present.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationhcodes H412 pcodes P273 - P501 RIDADR NONH for all modes of transport Узнать цену -
Galactose and Lactose Assay Kit MAK011-1KT
NACRES: NA.84 General descriptionLactose is a disaccharide containing one galactose and one glucose molecule. Lactose is the major sugar in the milk of most species, typically present between 2-8%. The enzyme lactase hydrolyzes lactose to its constituent monosaccharides. Galactose is a simple monosaccharide that serves as an energy source and as an essential component of glycolipids and glycoproteins. Galactose contributes to energy metabolism via its conversion to glucose by the enzymes that constitute the Leloir pathway. Defects in the genes encoding these proteins leads to the metabolic disorder galactosemia.ApplicationGalactose and lactose assay kit has been used in the measurement of lactose/galactose concentration in milk/lactose-free milk and to estimate the saccharides concentrations measured with the biosensor.SuitabilitySuitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.PrincipleIn this assay kit, galactose is oxidized by galactose oxidase resulting in a colorimetric (570 nm)/ fluorometric (ex = 535 nm/em = 587 nm) product, proportional to the galactose present. To detect lactose, lactose is hydrolyzed by lactase to generate free galactose, which is then measured (total galactose). The lactose level is equal to total galactose minus free galactose.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 - H412 pcodes P261 - P272 - P273 - P280 - P284 - P302 + P352 RIDADR NA 1993 / PGIII Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Galactose Assay Kit MAK012-1KT
NACRES: NA.84 General descriptionGalactose is a simple monosaccharide that serves as an energy source and as an essential component of glycolipids and glycoproteins. Galactose contributes to energy metabolism via its conversion to glucose by the enzymes that constitute the Leloir pathway. Defects in the genes encoding these proteins lead to the metabolic disorder galactosemia.Application- Galactose assay kit has been used in the determination of reactor galactose concentration.
- It has been used in D-galactose assay using the galactose oxidase enzymatic microreactor.
- It has also been used in the general synthesis and characterization of O-(2-aminoethyl)-O-galactosyl polyethylene glycol (H2N-PEG-GAL) derivative.
SuitabilityThis kit is suitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.PrincipleIn this assay kit, Galactose is oxidized resulting in a colorimetric (570 nm)/fluorometric (ex = 535 nm/em = 587 nm) product, proportional to the galactose present. This kit has a linear range of detection between 0.2-1.0 nmole galactose for the fluorometric assay and 2-10 nmole galactose for the colorimetric assay.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 - H412 pcodes P261 - P272 - P273 - P280 - P284 - P302 + P352 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
GAPDH Activity Assay Kit MAK277-1KT
NACRES: NA.84 General descriptionGAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology.ApplicationGAPDH Activity Assay Kit has been used to measure the enzyme activity of glyceraldehyde-3-phosphate dehydrogenase in testis.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for use with various tissue and cell culture samples.PrincipleThe GAPDH Activity Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various samples. GAPDH activity is determined in a coupled enzyme reaction in which GAP is converted to BPG by GAPDH. This results in a colorimetric (450 nm) product proportional to the enzymatic activity present. The assay is sensitive to 100 mUnits/mL. One unit of GAPDH activity is the amount of enzyme that will generate 1.0 mmole of NADH per minute at pH 7.2 at 37 °C.Параметрыdetection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05 signalword Danger hcodes H314 - H412 pcodes P273 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric) MAK348-1KT
General descriptionGelatinases are a type of matrix zinc-dependent metalloproteases (MMPs) that degrade gelatins and a variety of other extracellular matrix proteins. These enzymes are synthesized as latent zymogens that are activated by proteolysis and inhibited by tissue inhibitors of metalloproteases (TIMPs). Two mammalian gelatinases, Gelatinase A (MMP-2) and Gelatinase B (MMP-9), are critical for basement membrane degradation and are highly upregulated in variety of tumor cells.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the detection of gelatinase activity in biological samples such as recombinant protein, tissue, cell lysates, etcPrincipleThe Gelatinase Activity Assay Kit utilizes a hybrid approach for the detection of gelatinase activity by employing a highly quenched gelatin substrate which upon cleavage by a suitable gelatinase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of gelatinase activity (as low as 0.06 mCDU).Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS09 hcodes H411 pcodes P273 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Glucose (GO) Assay Kit GAGO20-1KT
NACRES: NA.84 ApplicationThis kit is for the quantitative, enzymatic determination of glucose in food and other materials. Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product. The intensity of the pink color measured at 540 nm is proportional to the original glucose concentration.SuitabilitySuitable for the quantitative, enzymatic determination of glucosein food and other materials.Параметрыusage sufficient for 20 assays Quality Level 100 Featured Industry Food and Beverages
General Analyticalstorage temp. 2-8°C
Safety InformationPictograms GHS07,GHS08 Signal Word Danger Hazard Statements H302 - H350 Precautionary Statements P202 - P264 - P270 - P280 - P301 + P312 - P308 + P313 RIDADR NONH for all modes of transport Узнать цену -
Glucose and Sucrose Assay Kit MAK013-1KT
NACRES: NA.84 General descriptionSucrose is a disaccharide that is hydrolyzed to glucose and fructose by invertase. Glucose is the primary carbohydrate utilized for energy during cellular respiration. Upon cellular uptake, glucose can be shunted towards breakdown via glycolysis or polymerized for energy storage.ApplicationGlucose and sucrose assay kit has been used to:- determine sucrose, aerobic glycolysis and pyruvate kinase activity
- determine protein and sugar in strawberries
- determine plasma insulin and glucose levels in mice
- analyze plasma glucose and plasma lactate and to quantify glucose/sucrose.
SuitabilitySuitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.PrincipleIn this assay kit, glucose is oxidized via glucose oxidase resulting in a colorimetric (570 nm)/ fluorometric (ex = 535 nm/em = 587 nm) product, proportional to the glucose present. To measure sucrose, invertase is added to the reaction to convert the sucrose to glucose and fructose. The free glucose can be subtracted from the total glucose to give the concentration of sucrose present. This kit has a linear range of detection between 0.2-1.0 nmole of glucose for the fluorometric assay and 2-10 nmoles of glucose for the colorimetric assay.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 - H412 pcodes P261 - P273 - P284 - P501 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Glucose Colorimetric/Fluorometric Assay Kit MAK263-1KT
NACRES: NA.84 General descriptionGlucose is a primary energy source that naturally occurs in its free state in fruits and other plant parts. Abnormal glucose levels have been associated with several metabolic dysfunctions such as hypoglycemia, hyperglycemia, and diabetes mellitus. Measurements of glucose levels in tissues and body fluids (such as blood and urine) are often used for the diagnosis of glucose– related disorders. Glucose levels are also monitored to check the efficacy of therapeutics such as insulin and sulphonylureas in type 2 diabetics.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the determination of glucose concentrations in various biological samples, including serum, plasma, food, or growth medium. This kit is also suitable for monitoring glucose levels during fermentation and glucose feeding in protein expression processes.PrincipleGlucose is oxidized to generate a colorimetric (570 nm)/ fluorometric (ex = 535/em = 587 nm) product, proportional to the amount of glucose present. The kit is able to detect 1-10,000 µM of glucose in various samples.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 - H412 pcodes P261 - P273 - P284 - P501 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Glucose Oxidase Activity Assay Kit MAK097-1KT
NACRES: NA.84 General descriptionGlucose Oxidase (GOx, EC 1.1.3.4) is an enzyme found in insects, fungi, and bacteria that catalyzes the oxidation of D-glucose to D-gluconolactone. GOx is widely used in the food, beverage, chemical, and pharmaceutical industries.SuitabilitySuitable for the measurement of glucose oxidase (GOx) in a variety of samples including cells and serumPrincipleThe Glucose Oxidase Activity Assay Kit provides a simple and direct procedure for measuring GOx activity in a variety of biological samples. GOx activity is determined by a coupled enzyme assay, in which GOx oxidizes D-glucose resulting in the production of hydrogen peroxide (H2O2) that reacts with a probe, generating a colorimetric (570 nm)/fluorometric (ex = 535/em = 587 nm) product, proportional to the GOx present. One unit of GOx is defined as the amount of enzyme that generates 1.0 µmole of H2O2 per minute at 37 °C.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 - H412 pcodes P261 - P272 - P273 - P280 - P284 - P302 + P352 RIDADR UN 1993 / PGIII Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Glucose Uptake Colorimetric Assay Kit MAK083-1KT
General descriptionGlucose is the primary source of energy for most cells. Glucose uptake into cells is highly regulated and the first rate limiting step in glucose metabolism. Glucose uptake is facilitated by the GLUT family of transporter proteins, whose expression and activity are regulated by multiple mechanisms. Glucose uptake is upregulated in many cancer cells, which exhibit high rates of aerobic glycolysis. Cells exhibiting insulin resistance show diminished glucose uptake in response to insulin stimulation.
The use of the recycling amplification reaction in the colorimetric assay results in the limit of detection being 10-fold lower (20-100 pmole) compared to the fluorescence assay (200-1,000 pmole, MAK084).ApplicationGlucose uptake colorimetric assay kit has been used to measure glucose uptake by a variety of cells.SuitabilitySuitable for detecting glucose uptake in adherent or suspension cells cultured in a 96-well microtiter plate.PrincipleThe Glucose Uptake Colorimetric Assay kit provides a simple and direct procedure for measuring glucose uptake in a variety of cells. Glucose uptake is measured using the glucose analog, 2-deoxyglucose (2-DG), which is taken up by cells and phosphorylated by hexokinase to 2-DG6P. 2-DG6P cannot be further metabolized and accumulates in cells, directly proportional to the glucose uptake by cells. In this assay, 2-DG uptake is determined by a coupled enzymatic assay in which the 2-DG6P is oxidized, resulting in the generation of NADPH, which is then determined by a recycling amplification reaction in which the NADPH is utilized by glutathione reductase in a coupled enzymatic reaction that produces glutathione. Glutathione reacts with DTNB to product TNB, which is detected at 412 nm.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS09 hcodes H411 pcodes P273 RIDADR NONH for all modes of transport Узнать цену -
Glucose Uptake Fluorometric Assay Kit MAK084-1KT
NACRES: NA.84 General descriptionGlucose is the primary source of energy for most cells.Transport of glucose across the plasma membrane is the first rate limiting step in glucose metabolism. Glucose uptake is facilitated by the GLUT family of transporter proteins, whose expression and activity are regulated by multiple mechanisms. Glucose uptake is upregulated in many cancer cells, which exhibit high rates of aerobic glycolysis. Cells exhibiting insulin resistance show diminished glucose uptake in response to insulin stimulation.ApplicationGlucose Uptake Fluorometric Assay Kit has been used to measure the amount of glucose uptake by monocytic myeloid-derived suppressor cells (M-MDSCs).SuitabilitySuitable for detecting glucose uptake in adherent or suspension cells cultured in a 96-well microtiter plate.PrincipleThe Glucose Uptake Fluorometric Assay kit provides a simple and direct procedure for measuring glucose uptake in a variety of cells. Glucose uptake is measured using the glucose analog, 2-deoxyglucose (2-DG), which is taken up by cells and phosphorylated by hexokinase to 2-DG6P. 2-DG6P cannot be further metabolized and accumulates in cells, directly proportional to the glucose uptake by cells. In this assay, 2-DG uptake is determined by a coupled enzymatic assay in which the 2-DG6P is oxidized, resulting in the generation of NADPH, which reacts with the probe to generate a fluorometric (λex = 535/λem = 587 nm) product, proportional to the 2-DG taken up by the cell.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H319 - H335 - H412 pcodes P261 - P264 - P271 - P273 - P302 + P352 - P305 + P351 + P338 Target Organs Respiratory system RIDADR UN 1993 / PGIII Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Glucose-1-Phosphate (G1P) Colorimetric Assay Kit MAK098-1KT
NACRES: NA.84 General descriptionGlucose-1-phosphate (G1P), also known as the Cori ester due to its discovery by Gerty and Carl Cori, is produced from glycogen during glycogenolysis by the actions of glycogen phosphorylase. Conversion to glucose-6-phosphate (G6P) by phosphoglucomutase allows for entry of the glucose molecule into metabolic pathways such as glycolysis. During glycogenesis, G6P is converted to G1P by the actions of phosphoglucose isomerase.SuitabilitySuitable for the measurement of glucose-1-phosphate in various tissues (liver, muscle and heart etc.) and cells (adherent or suspension), the analysis of glucose metabolism and cell signaling in various cells and screening anti-diabetic drugs.PrincipleThe G1P Colorimetric Assay kit provides a simple and direct procedure for measuring G1P in a variety of samples. G1P concentration is determined by a coupled enzyme assay, in which G1P is converted to glucose-6-phosphate by phosphoglucomutase in the presence of glucose-1,6-biphosphate. The glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH. The resulting NADH reduces a colorless probe resulting in a colorimetric (450 nm) product proportional to the G1P present.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Glucose-6-Phosphate Assay Kit MAK014-1KT
NACRES: NA.84 General descriptionGlucose 6-phosphate (G6P) is a key metabolic intermediate that enters either metabolic pathways or storage. G6P is generated when glucose is phosphorylated by hexokinase or glucokinase or by the conversion of glucose-1-phosphate by phosphoglucomutase during glycogenolysis. G6P lies at the beginning of both glycolysis and the pentose phosphate pathways. It also can be stored as glycogen when blood glucose levels are high.ApplicationGlucose-6-phosphate assay kit has been used in:- glucose 6-phosphate (G6P) assay
- quantification of glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P)
- the determination of malate
- metabolite analysis
SuitabilitySuitable for quantifying Glucose-6-Phosphate in a variety of samples.PrincipleG6P is utilized by Glucose-6-phosphate dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. G6P is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional to the G6P present. Typical sensitivities of detection are between 1-30 nmoles.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Glucose-6-Phosphate Dehydrogenase Activity Assay Kit MAK015-1KT
General descriptionGlucose-6-phosphate dehydrogenase (G6PDH) catalyzes the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step of the pentose phosphate pathway (PPP). The PPP pathway critical for maintaining the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH) and for the production of pentose sugars. The NADPH produced is critical for redox regulation via the regeneration of GSH and for providing reducing equivalents for fatty acid biosynthesis. Deficiencies in G6PDH predisposes individuals to non-immune hemolytic anemia.ApplicationGlucose-6-phosphate dehydrogenase assay kit has been used in the determination of glucose-6-phosphate dehydrogenase (G6PDH) activity and to measure PDH (pyruvate dehydrogenase).SuitabilitySuitable for the measurement of Glucose-6-phosphate dehydrogenase (G6PDH) activity in various tissues and cells.PrincipleIn the glucose-6-phosphate dehydrogenase assay kit, glucose-6-phosphate is oxidized to generate a product which is specifically detected by colorimetric (450 nm) assay. The G6PDH Assay Kit can detect G6PDH activity as low as 0.04mU G6PDH per well.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. 20°C Gene Information human ... H6PD(9563)
mouse ... H6PD(100198)
rat ... H6PD(298655)
Safety Informationpictograms GHS05 signalword Danger hcodes H314 pcodes P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 RIDADR NONH for all modes of transport Узнать цену -
Glutamate Assay Kit MAK330-1KT
General descriptionGlutamate is an important chemical in general metabolism. It is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis, lathyrism, autism and Alzheimers disease. Glutamate is also widely used as a flavor enhancer in the food industry. Simple, direct and automation-ready procedures for measuring glutamate concentration are very desirable.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for glutamate determination in serum, plasma, tissue extracts and food extract samples. Also suitable in drug discovery/pharmacology for studying the effects of drugs on glutamate levels.PrincipleThe Glutamate Assay Kit is based on glutamate dehydrogenase catalyzed oxidation of glutamate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the glutamate concentration in the sample. Detection limit of 50 μM , linearity up to 2.5 mM glutamate in 96-well plate assay.Параметрыdetection method colorimetric storage temp. −20°C Узнать цену -
Glutamate Assay Kit MAK004-1KT
NACRES: NA.84 General descriptionGlutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. The synthesis of α-KG from glutamate by glutamate dehydrogenase is a key TCA cycle anaplerotic reaction. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Within the mammalian nervous system, glutamate is the most abundant excitatory neurotransmitter. It is believed to be involved in learning and memory and may also be involved in diseases like amyotrophic lateral sclerosis, autism, some forms of mental retardation, and Alzheimer′s disease. Glutamate is present in a wide variety of foods, and has been used as a flavor enhancer in food industry.ApplicationGlutamate Assay Kit has been used to determine the concentration of glutamate in cerebrospinal fluid in a rat subarachnoid hemorrhage model. It has also been used to determine cytosolic and secreted glutamate concentrations in cerebrospinal fluid of patients with amyotrophic lateral sclerosis.SuitabilitySuitable to determine glutamate concentration in a variety of samples.PrincipleGlutamate concentration is determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the glutamate present.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS09 hcodes H411 pcodes P273 - P391 - P501 RIDADR NONH for all modes of transport WGK Germany WGK 3 Узнать цену -
Glutamate Dehydrogenase (GDH) Activity Assay Kit MAK099-1KT
General descriptionGlutamate Dehydrogenase (GDH) is a mitochondrial enzyme that catalyzes the reversible oxidative deamination of glutamate to α-ketoglutarate and serves as a key link between anabolic and catabolic pathways. In mammals, GDH is subject to allosteric regulation and has high activity in liver, kidney, brain, and pancreas. GDH activity in serum can be used to differentiate between liver diseases due to liver inflammation, which do not show elevated serum GDH activity, and diseases that result in hepatocyte necrosis, which results in elevated serum GDH.ApplicationGlutamate Dehydrogenase (GDH) Activity Assay Kit has been used to determine the enzymatic activity of glutamate dehydrogenase.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for detecting glutamate dehydrogenase (GDH) activity in serum or tissue and cell extracts.PrincipleGDH activity is determined by a coupled enzyme assay in which glutamate is consumed by GDH generating NADH, which reacts with a probe generating a colorimetric (450 nm) product proportional to the GDH activity present. One unit of GDH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 7.6 at 37 °C.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. 20°C Gene Information human ... GLUD1(2746), GLUD2(2747)
mouse ... GLUD1(14661)
rat ... GLUD1(24399)
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Glutamate Oxidase Assay Kit MAK170-1KT
EC Number: 200-664-3 NACRES: NA.84 General descriptionGlutamate oxidase is an oxidoreductase that catalyzes the oxidative deamination of L-glutamate to ketoglutarate, ammonia and hydrogen peroxide.
Glutamate oxidase is an oxidoreductase that catalyzes the oxidative deamination of L-glutamate to ketoglutarate, ammonia, and hydrogen peroxide.
The Glutamate Oxidase Assay Kit provides a simple and direct procedure for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts. Glutamate Oxidase activity is determined by a coupled enzyme assay, in which the oxidation of glutamic acid results in a fluorescent product (ex =540/em = 590 nm) proportional to the glutamate oxidase activity present. One unit of glutamate oxidase activity is defined as the amount of enzyme that catalyzes the conversion of L-glutamic acid to 1.0 µmole a-ketoglutaric acid per minute at pH 7.4 at room temperature.SuitabilitySuitable for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts.PrincipleThe glutamate oxidase assay kit provides a simple and direct procedure for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts. Glutamate oxidase activity is determined by a coupled enzyme assay, in which the oxidation of glutamic acid results in a fluorescent product (ex =540/em = 590 nm) proportional to the glutamate oxidase activity present. One unit of glutamate oxidase activity is defined as the amount of enzyme that catalyzes the conversion of L-glutamic acid to 1.0µmole -ketoglutaric acid per minute at pH 7.4 at room temperature.Параметрыusage sufficient for 200 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 pcodes P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312 RIDADR NONH for all modes of transport Узнать цену -
Glutathione Peroxidase Cellular Activity Assay Kit
NACRES: NA.32
Кат. номер CGP1-1KT CGP1-1KT-PW General descriptionThe Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.SuitabilitySuitable for the measureent of glutathione peroxidase in tissue extractsPrincipleThe Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.Other NotesThis kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme.ПараметрыQuality Level 200, usage sufficient for 100 tests shelf life Kit is stable for 24 months when unopened. detection method colorimetric shipped in wet ice storage temp. −20°C
Safety Informationpictograms GHS02,GHS05,GHS06,GHS08,GHS09 signalword Danger hcodes H226 - H242 - H302 + H332 - H311 - H314 - H341 - H411 pcodes P210 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 - P370 + P378 RIDADR UN 3316 9 WGK Germany WGK 3 Узнать цену -
Glutathione Reductase Assay Kit
Кат. номер GRSA-1KT GRSA-1KT-PW General descriptionGlutathione reductase (GR) is a ubiquitous enzyme that catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). Glutathione reductase is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also a substrate for the glutathione peroxidases and glutathione S-transferases in the detoxification of organic peroxides and the metabolism of xenobiotics.ApplicationGlutathione Reductase Assay Kit has been used to measure the activity of glutathione reductase as a part of oxidative stress assessment and also to study the effects of antifouling biocides on it.Biochem/physiol ActionsGlutathione reductase (EC 1.6.4.2) (GR) is a ubiquitous enzyme that catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). Glutathione reductase is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also a substrate for the glutathione peroxidases and glutathione S-transferases in the detoxification of organic peroxides and the metabolism of xenobiotics. This kit contains reagents for the spectrophotometric determination of glutathione reductase activity either by following the decrease in absorbance caused by the oxidation of NADPH at 340 nm (UV assay) or the increase in absorption caused by the reduction of dithiobis(2-nitrobenzoic acid) (DTNB) at 412 nm (colorimetric assay). This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid).
This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid).SuitabilitySuitable for the measurement of glutathione reductase activity in biological samplesPrincipleThis kit contains reagents for the spectrophotometric determination of glutathione reductase activity either by following the decrease in absorbance caused by the oxidation of NADPH at 340 nm (UV assay) or the increase in absorption caused by the reduction of dithiobis(2-nitrobenzoic acid) (DTNB) at 412 nm (colorimetric assay). This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid).Analysis NoteUV assay: One unit will cause the oxidation of 1.0 µmole of NADPH at 25 °C at pH 7.5.
Colorimetric assay: One unit will cause the reduction of 1.0 µmole of DTNB to TNB at 25 °C at pH 7.5.ПараметрыQuality Level 200, usage sufficient for 100 tests detection method colorimetric storage temp. 2-8°C Gene Information human ... GSR(2936)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Glycerol Assay Kit MAK117-1KT
NACRES: NA.84 General descriptionGlycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides. Glycerol is a sweet-tasting, hygroscopic liquid. Glycerol is widely used in food, beverage, and pharmaceutical formulations and is the main waste by-product of biodiesel production via transesterification.ApplicationGlycerol assay kit has been used to quantify glycerol release from mouse 3T3-L1 cell culture supernatant samples and to measure plasma glycerol levels.Features and BenefitsCompatible with high-throughput handling systems.SuitabilityThis kit is suitable for glycerol detection in foods, beverages, pharmaceutical formulations, and biological samples.PrincipleIn this kit, glycerol concentration is determined by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the glycerol present. The linear range of detection for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).Параметрыusage sufficient for 200 colorimetric or fluorometric tests detection method colorimetric, fluorometric shipped in dry ice storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Glycerol-3-Phosphate Assay Kit MAK207-1KT
EC Number: 249-889-9 NACRES: NA.84 General descriptionGlycerol 3-phosphate (G3P) is an important intermediate of carbohydrate and lipid metabolic pathways. It is produced from glycerol by glycerol kinase or from dihydroxyacetone phosphate by glycerol 3-phosphate dehydrogenase. G3P may enter the G3P shuttle to generate NAD+, or may be converted to glyceraldehyde 3-phosphate and enter glycolysis or the lipid biosynthesis pathway.ApplicationGlycerol-3-Phosphate Assay Kit has been used in the determination of glycerol-3-phosphate and phosphoenolpyruvate levels. It has also been used to quantify the concentration in aerial mycelium.SuitabilitySuitable for the detection of Glycerol-3-Phosphate in tissue and cell culture samples.PrincipleThe Glycerol 3-Phosphate Colorimetric Assay Kit provides a simple assay for measuring G3P in various tissues and cells (ranging from 2-10 nmole/well). G3P is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the amount of G3P present.Параметрыdetection method colorimetric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Glycine Assay Kit (Fluorometric) MAK261-1KT
NACRES: NA.84 General descriptionGlycine (GLY) is a non-essential amino acid used to help create muscle tissue and convert glucose into energy. It′s basic functions include the participation in the synthesis of creatine, glutathione, heme groups, and conjugated bile acids (bile salts). It is also used in the synthesis of DNA and RNA. It is one of the most abundant residues in the triple-helical structure of collagen, which contributes to the elasticity of skin and connective tissue. It acts as a glucogenic amino acid regulating blood sugar levels. Glycine is necessary for central nervous system function. It possesses both inhibitory and excitatory neurotransmitter functions in the brain stem and spinal cord. Recent studies suggest, glycine may play a role in tumorigenesis and malignancy. Some of the external sources of glycine includes legumes, meat, fish and dairy products.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the detection of glycine in biological fluids such as serum, plasma, saliva, urine, etcPrincipleThe Glycine Assay Kit is a simple and reliable, high throughput assay that detects the physiological concentration of glycine in a variety of biological fluids. In the assay, glycine is oxidized generating a fluorometric (lex = 535 nm/ lem = 587 nm) product, proportional to the glycine present. The assay is specific, and other standard and non-standard amino acids do not interfere with the assay. The kit is sensitive to 1 mM of glycine.Параметрыdetection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS05,GHS07 signalword Danger hcodes H290 - H314 - H335 pcodes P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Target Organs Respiratory system RIDADR UN 3316 9 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Glycogen Assay Kit MAK016-1KT
NACRES: NA.84 General descriptionGlycogen is a branched polymer of glucose that serves as the primary short-term energy storage molecule in animals. Glycogen is primarily synthesized in liver and muscle tissue where it can constitute up to 10% of the weight of liver and 1-2% of the weight of muscle tissue. While muscle glycogen is generally utilized locally, liver glycogen serves as an important buffer to regulate blood glucose levels. Glycogen metabolism is dysregulated in diabetes and the glycogen storage diseases due to inborn errors of metabolism.ApplicationGlycogen Assay Kit has been used in glycogen quantification. It has also been used to determine the glycogen content in liver, vastus lateralis and muscle homogenates.SuitabilitySuitable for determining glycogen concentration in various tissues such as liver etc. and cell culture (adherent or suspension cells).PrincipleGlycogen concentration is determined by a coupled enzyme assay, which produces a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the glycogen present.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) 188.6 °F - closed cup Flash Point(C) 87 °C - closed cup Узнать цену -
Glycogen Phosphorylase Colorimetric Assay Kit MAK417-1KT
General descriptionGlycogen phosphorylase catalyzes the rate-limiting step in glycogenolysis using glycogen and inorganic phosphate to produce glucose-1-phosphate (G1P). In mammals, glycogen phosphorylase is abundant in muscle, liver, and brain tissues. There are two forms of glycogen phosphorylase, namely glycogen phosphorylase a and b forms. Glycogen phosphorylase a is the highly active form whereas glycogen phosphorylase b has only limited activity. Glycogen phosphorylase is a clinically significant enzyme as its mutations are associated with different glycogen storage diseases in muscle and liver. In addition, this enzyme has been suggested as a biomarker for gastric cancer and its inhibition has been tested in treating type 2 diabetes.Features and BenefitsGlycogen Phosphorylase Assay Kit:- Detects as low as 10 mU in a variety of sample types.
SuitabilityThe kit is suitable for the measurement of glycogen phosphorylase activity in cell culture and animal tissues (heart, liver, kidney, muscle, etc.) as well as the analysis of glycogen phosphorylase kinetics, inhibition or activation.PrincipleThe Glycogen Phosphorylase Colorimetric Activity Assay Kit is based on the detection of G1P by a set of enzymatic reactions to generate a colored product with a strong absorbance at A450 nm. The A450 nm signal is directly proportional to the glycogen phosphorylase activity. The kit is a rapid, sensitive and convenient tool for detecting glycogen phosphorylase activity.ПараметрыQuality Level 100, detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05 signalword Danger hcodes H314 - H412 pcodes P273 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Узнать цену
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