Tyrosinase Activity Assay Kit (Colorimetric) MAK395-1KT

Tyrosinase is a copper-binding enzyme that is expressed across a vast range of species ranging from bacteria and fungi to mammals. It is involved in two sequential reactions of the melanin synthesis pathway; first being the hydroxylation of a monophenol and second the conversion of an ortho-diphenol to a quinone. Quinone then undergoes a series of reactions including polymerization to form melanin. Tyrosinase is of great interest to the agriculture industry since it causes browning of fruits, vegetable and mushrooms, as well as to the cosmetic industry as it causes skin darkening. Development and screening of tyrosinase inhibitors, therefore, is very useful for conditions such as hyperpigmentation and melasma. Tyrosinase activity is significantly increased in melanoma. Therefore, the detection of tyrosinase activity could be promising as a specific diagnostic test for melanoma and may be useful in monitoring patient response to melanoma treatments.
Tyrosinase Activity Assay Kit:

• Non-radioactive assay.
• Sensitive, detecting Tyrosinase Activity 30 µU.

The kit is suitable for the measurement of tyrosinase activity in cell lysates (e.g. Melanoma cells), tissue lysates, plant tissue lysates (e.g. potato), recombinant enzyme and purified protein.
In this assay, tyrosinase catalyzes the conversion of a phenolic substrate to a Quinone intermediate, which reacts with the tyrosine enhancer forming a highly stable chromophore with absorbance at A510 nm. The assay can detect as low as 30mU Tyrosinase in biological samples.

Quality Level	100,
detection method	colorimetric
storage temp.	−20°C

pictograms	GHS06
signalword	Danger
hcodes	H301 - H315 - H319 - H335
pcodes	P261 - P264 - P270 - P301 + P310 - P302 + P352 - P305 + P351 + P338
Target Organs	Respiratory system