Thioredoxin Reductase Kit MAK409-1KT

Thioredoxin reductase (TrxR) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide.
Thioredoxin Reductase Kit:

• Simple and fast procedure with results in 40 minutes.

The kit is suitable for the measurement of thioredoxin reductase (TrxR) in various samples including tissue, cells and serum.
In the assay TrxR catalyzes the reduction of 5, 5-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generates a strong yellow color at A412 nm. In crude biological samples, other enzymes such as glutathione reductase and glutathione peroxidase can also reduce DTNB. Therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed. The first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR.

Quality Level	100,
detection method	colorimetric
storage temp.	−20°C

pictograms	GHS05
signalword	Danger
hcodes	H315 - H318
pcodes	P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364