Phosphoglycerate Mutase Activity Assay Kit (Colorimetric/Fluorometric) MAK424-1KT

Phosphoglycerate Mutase (PGAM) is an important enzyme in the glycolytic pathway that catalyzes the reversible conversion of 3-phosphoglycerate to 2-phosphoglycerate. It plays an important role in regulating glycolysis and anabolic activity to promote cancer cell proliferation. Several studies reported that PGAM is upregulated in many human cancers. Inhibition of this enzyme could lead to tumor cell death thereby making PGAM an attractive therapeutic target.
Phosphoglycerate Mutase Activity Assay Kit:

• Sensitive method to detect PGAM activity as low as 0.05 mU in a variety of samples.

The kit is suitable for the measurement of PGAM activity in various tissues (liver, muscle, heart etc.) and cells (adherent or suspension cells), the analysis of glycolytic metabolism in different sample types, the mechanistic studies of various cancers and for the screening of anti-cancer drugs.
The kit utilizes the ability of an active PGAM to catalyze the conversion of 3-Phosphoglycerate to 2-Phosphoglycerate (2-PG), which is subsequently used to generate an intermediate product. The intermediate product then stoichiometrically reacts with a probe to generate color (A570 nm) or fluorescence (ex = 535 nm/em = 587 nm).

Quality Level	100,
detection method	colorimetric, fluorometric
storage temp.	−20°C

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H315 - H317 - H319 - H334 - H335
pcodes	P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
Target Organs	Respiratory system