NNMT Inhibitor Screening Assay Kit MAK299-1KT

NACRES:

NA.84

N′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy.
Suitable for screening/studying/characterizing potential inhibitors of N′-Nicotinamide Methyltransferase (NNMT).
This NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using the Thiol Detecting Probe, generating an enhanced fluorescence signal (λex = 392 nm/ λem = 482 nm). In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.

detection method	fluorometric
storage temp.	−70°C

pictograms	GHS02,GHS05,GHS07,GHS08
signalword	Danger
hcodes	H226 - H290 - H312 - H314 - H317 - H361fd - H412
pcodes	P201 - P210 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 + P310
RIDADR	UN 3316 9
Flash Point F	84.2 °F
Flash Point C	29 °C