Homocysteine Assay Kit MAK354-1KT

Homocysteine is a non-proteogenic amino acid synthesized intracellularly by removal of the N-methyl group from the essential amino acid methionine. Homocysteine is exported from cells into the blood, where it exists mainly as an oxidized disulfide species, either as a dimer or bound to cysteine residues of serum proteins. The reduced form of homocysteine (‘free homocysteine) can be metabolized into cysteine via the transsulfuration pathway; however, it can also undergo intramolecular cyclization, forming the highly reactive pro-oxidant homocysteine thiolactone. Subsequent N-homocysteinylation of protein lysine residues by the reactive thiolactone disrupts protein conformation, leading to formation of cytotoxic protein aggregates. Homocysteinylated proteins may also act as autoantigens, triggering arterial inflammation and atherosclerosis. Elevated plasma homocysteine concentration is a clinical biomarker for increased risk of cardiovascular disease, ischemic stroke and myocardial infarction. Severely elevated homocysteine levels (hyperhomocysteinemia) are correlated with a 4-fold increase in mortality due to heart attack and a 16-fold increase in the likelihood of recurrent stroke.
Compatible with high-throughput handling systems.
Suitable for the determination of total homocysteine concentration in plasma or serum.
The Homocysteine Assay Kit allows for quantification of total homocysteine in biological fluids such as plasma and serum. The assay is based on the reduction of homocysteine disulfides to free homocysteine, which is cleaved by a homocysteine-selective enzyme, generating an intermediate product. The intermediate reacts with a probe solution to form a stable fluorophore that emits in the far-red spectrum (λex = 658 nm/λem = 708 nm). The assay is not affected by physiological concentrations of other biological thiols (such as cysteine, methionine and glutathione), is high-throughput adaptable and can detect as low as 5 μM homocysteine.

usage	sufficient for 100 fluorometric tests
detection method	fluorometric

pictograms	GHS05,GHS09
signalword	Warning
hcodes	H290 - H315 - H319 - H411
pcodes	P234 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
Flash Point F	Not applicable
Flash Point C	Not applicable