Fluorometric Sphingomyelinase Assay Kit MAK155-1KT

NACRES:

NA.84

Sphingomyelinase (SMase), or sphingomyelin phosphodiesterase, catalyzes the hydrolytic cleavage of sphingolipid to phosphocholine and ceramide, a second messenger involved in multiple cellular responses including the cellular response to stress. There are at least five types of SMases identified, which are classified according to their cation dependence, cellular location, and pH optima for activity, and include the acidic and neutral sphingomyelinase. Neutral SMases, along with the acidic SMases, are thought to be major contributors to the production of ceramide in response to cellular stress. Mutations in is associated with metabolic disorders like Niemann-Pick disease B.
This kit is suitable for the detection of sphingomyelin in a variety of samples such as blood and cell extracts.
The Sphingomyelinase Assay Kit provides a simple and direct procedure for measuring neutral SMase activity in a variety of samples such as blood or cell extracts, or for screening SMase activators and inhibitors. SMase activity is determined by a coupled enzyme assay, which results in a fluorescent product (λex =540/λem = 590 nm) proportional to the SMase activity present.

usage	sufficient for 200 fluorometric tests (red fluorescence)
detection method	fluorometric
storage temp.	20°C
Gene Information	human ... SMPD2(6610), SMPD3(55512), SMPD4(55627) mouse ... SMPD2(20598), SMPD3(58994), SMPD4(77626) rat ... SMPD2(83537), SMPD3(94338), SMPD4(303790)

pictograms	GHS05,GHS08,GHS09
signalword	Danger
hcodes	H317 - H318 - H334 - H410
pcodes	P261 - P273 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
RIDADR	NONH for all modes of transport