Deproteinizing Sample Preparation Kit II MAK342-1KT

The study of biological fluids, cell, bacteria, yeast, and tissue lysates has delivered vital information regarding their biochemical composition. These biological samples comprise a rich, complex list of lipids, proteins, small organic molecules, ions, and, depending on the sample, exogenous molecules (i.e. drugs). Several small organic molecules can also be found free and/or bound to other macromolecules. These macromolecules (lipoproteins, proteins, enzymes) interfere or degrade low molecular-weight metabolites. Therefore, sample deproteinization is often required. Chemical deproteinization procedure such as Trichloroacetic acid (TCA) is one of the most utilized methods to accomplish sample deproteinization.
Suitable for deproteinizing biological fluids (serum, plasma, whole blood, saliva, urine etc.) and lysates (cell culture, mammalian tissue, yeast, bacteria etc.)
The Deproteinizing Sample Preparation Kit II offers an excellent alternative when metabolite lability affects the accurate detection and estimation of small molecule concentrations and when organic solvents or ultrafiltration cannot be used. TCA precipitates proteins by lowering the sample pH drastically. After removal of precipitated proteins, the pH of the sample is neutralized with neutralization buffer that is provided in the kit.

usage

sufficient for 200 assays

pictograms	GHS05,GHS07,GHS09
signalword	Danger
hcodes	H290 - H302 + H332 - H314 - H335 - H410
pcodes	P273 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 + P310
Target Organs	Respiratory system
Flash Point F	Not applicable
Flash Point C	Not applicable