Chymotrypsin Activity Assay Kit MAK345-1KT

Chymotrypsin is a key serine protease involved in dietary protein digestion in mammals. It is primarily produced by the pancreas, but may be expressed in other tissues, including spleen and liver. In the pancreas, chymotrypsin is initially expressed as the inactive proenzyme chymotrypsinogen, which is cleaved by other proteases to chymotrypsin, its active form. Chymotrypsin specifically cleaves peptide bonds at the C-terminal end of bulky hydrophobic or aromatic amino acids (such as tyrosine, tryptophan or phenylalanine).
Suitable for the measurement of chymotrypsin activity in tissue lysate or cultured cells
The Chymotrypsin Activity Assay kit uses a synthetic fluorogenic substrate, enabling kinetic measurement of chymotrypsin activity in cell and tissue lysates. A chymotrypsin activator cleaves chymotrypsinogen to form active chymotrypsin, which then hydrolyzes the non-fluorescent substrate to release a stable fluorophore (Ex/Em = 380/460 nm). The kit includes a selective chymotrypsin inhibitor that can be used to measure specific chymotrypsin activity in samples containing non-specific proteases and endopeptidases that may also metabolize the substrate. The assay can detect as low as 0.01 mU of Chymotrypsin.

usage	sufficient for 100 fluorometric tests
detection method	fluorometric
storage temp.	−20°C

pictograms	GHS08,GHS09
signalword	Danger
hcodes	H315 - H319 - H334 - H411
pcodes	P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
Flash Point F	188.6 °F
Flash Point C	87 °C