Mammalian Cell Lines
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RED.1
Кат. номер 85092601-1VL 85092601-DNA-5UG 85092601-RNA-5UG Cell Line OriginMouse leukaemic lymphoblastCell Line DescriptionA Rauscher virus induced erythroid leukaemia cell line. It is known to produce low amounts of Rauscher virus (a murine leukaemia virus).ApplicationTumourigenicity and virus studies: Rauscher virusCulture MediumDMEM or RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Let the cell culture media become acidic before sub-culturing/freezing. Limited lifespan in vitro, therefore a master bank should be frozen as soon as possible, of at least 10 ampoules.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse blood growth mode Suspension karyotype Aneuploid morphology Lymphoblast products Rauscher virus receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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REE, GD human cell line
Кат. номер 94082231-DNA-5UG 94082231-CDNA-20UL 94082231-1VL 94082231-RNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileHLA DRB1: 301
HLA DRB3: 101
HLA DQAP: 4.1
HLA DQA1: 501
HLA DQB1: 201
HLA DPB1: 01011 02012Serological ProfileHLA A: 1 24
HLA B: 8Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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REM 134 Cell Line canine 12122002-1VL
General descriptionREM 134 is a canine mammary carcinoma cell line derived from a spontaneous primary tumour.Culture MediumMEM (M2279), + 2mM L-Glutamine (G7513), + 10% FBS (F2442),Subculture RoutineSplit sub-confluent cultures (70-80%) seed new cultures at 2-4 x10,000 cells/cm using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply, for more information.CitationIf use of this culture results in a scientific publication, it should be cited in the publication as: REM 134 (ECACC 12122002)Параметрыbiological source canine mammary glands Quality Level 100, growth mode adherent morphology mixed population of polygonal cells application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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REM 885
Кат. номер 6070401-CDNA-20UL 6070401-DNA-5UG 6070401-RNA-5UG 6070401-1VL Cell Line OriginHuman EBV transformed lymphocytes AlzheimerCell Line DescriptionDerived from a patient with early onset Alzheimer disease and cerebral amyloid angiopathy (CAA), carrying a 6.5 Mb microduplication of the APP gene locus on chromosome 21. This is an EBV-transformed lymphoblastoid cell line.Culture MediumRPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 300,000 to 900,000 cells/ml; 5% CO2; 37°COther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lymph growth mode Suspension karyotype 46,XY.ish dup(21)(q21)(RP11-15D13++,RP11-66H5++) morphology Lymphoblastoid products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RENE 1
Кат. номер 92092510-1VL 92092510-DNA-5UG 92092510-RNA-5UG Cell Line OriginRat Sprague-Dawley, primary endometrial, adenovirus transformedCell Line DescriptionDerived from the endometrium tissue from the uterus of a 21-28 day old Sprague-Dawley rat. Cells carry vimentin, connexin 26 and connexin 43 markers. The transforming agent is adenovirus E1a protein.Culture MediumDMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time 48 hours.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat endometrium growth mode Adherent karyotype Not specified morphology Cuboidal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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RENE 2
Кат. номер 92092511-RNA-5UG 92092511-1VL 92092511-DNA-5UG Cell Line OriginRat Sprague-Dawley, primary endometrial, adenovirus transformedCell Line DescriptionDerived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. Cells carry vimentin, connexin 26 and connexin 43 markers. The transforming agent is adenovirus E1a protein.Culture MediumDMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time 48 hours.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat endometrium growth mode Adherent karyotype Not specified morphology Cuboidal products Not specified receptors Glucocorticoid application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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RENT 4
Кат. номер 92092512-DNA-5UG 92092512-1VL 92092512-RNA-5UG Cell Line OriginRat Sprague-Dawley, primary endometrial, SV40 large T antigen transformedCell Line DescriptionDerived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. Cells carry vimentin, connexin 43, collagen type III and proenkephalin markers. The transforming agent was SV40 large T antigen.Culture MediumDMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time 48 hours.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat endometrium growth mode Adherent karyotype Not specified morphology Spindle shaped products Not specified receptors Glucocorticoid application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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RENT RO1
Кат. номер 92092513-RNA-5UG 92092513-1VL 92092513-DNA-5UG Cell Line OriginRat Sprague-Dawley, primary endometrial, SV40 T antigen transformedCell Line DescriptionDerived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, connexin 43, desmin, proenkephalin, uteroglobin and uvomorulin markers.Culture MediumDMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time 35 hours.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat endometrium growth mode Adherent karyotype Not specified morphology Spindle shaped products Not specified receptors Glucocorticoid application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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RENT RO3
Кат. номер 92092514-1VL 92092514-DNA-5UG 92092514-RNA-5UG Cell Line OriginRat Sprague-Dawley, primary endometrial, SV40 T antigen transformedCell Line DescriptionDerived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, uvomorulin, proenkephalin, desmin and connexin 43 markers.Culture MediumDMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time 35 h.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat endometrium growth mode Adherent karyotype Not specified morphology Spindle shaped products Not specified receptors Glucocorticoid application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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RENT ts3.1
Кат. номер 92092516-1VL 92092516-RNA-5UG 92092516-DNA-5UG Cell Line OriginRat Sprague-Dawley, primary endometrial, SV40 T antigen transformedCell Line DescriptionDerived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat, transformed with a ts mutant of SV40 T antigen (tsA58). Cells express vimentin, CSF-1 (at 39°C), desmin, collagen type III and connexin 43.Culture MediumDMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 34°C. Doubling time 48 hours. Cells show continuous growth at 34°C and growth arrest at 39°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat endometrium growth mode Adherent karyotype Not specified morphology Cuboidal products Not specified receptors Glucocorticoid application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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RENT tsS1.1
Кат. номер 92092515-1VL 92092515-RNA-5UG 92092515-DNA-5UG Cell Line OriginRat Sprague-Dawley, primary endometrial, SV40 T antigen transformedCell Line DescriptionDerived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, CSF-1 (at 39°C), desmin, collagen type III and connexin 43 markers. The transforming agent is a ts mutant of SV 40 T-antigen (tsA58).Culture MediumDMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time 48 hours. Cells show continuous growth at 34°C and growth arrest at 39°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat endometrium growth mode Adherent karyotype Not specified morphology Cuboidal products Not specified receptors Glucocorticoid application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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RFAL3
Кат. номер 87100702-1VL 87100702-DNA-5UG 87100702-RNA-5UG Cell Line OriginMouse NS1/1 x Mouse BALB/c spleenCell Line DescriptionThe antibody is directed against 100kDa acute lymphoblastic leukaemia antigen, common acute lymphoblastic leukaemia and Daudi cells, glomeruli and proximal tubules in the kidney. The antibody used for bone-marrow purging in all suffering patients prior to autologous bone-marrow transplantation.ImmunogenNALM-6 cellsCulture MediumRPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS1/1 Reactivity Anti-CD10, human application(s) cell culture | mammalian: suitable isotype IgM shipped in dry ice storage temp. −196°C
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RFL-6
Кат. номер 86072501-RNA-5UG 86072501-1VL 86072501-DNA-5UG Cell Line OriginRat Sprague-Dawley lungCell Line DescriptionDerived from the lung tissue of a normal germ-free, 18 day gestation, Sprague-Dawley rat foetus. At least 45 population doublings expected.Culture MediumHam′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat lung growth mode Adherent karyotype 2n = 42 morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Ri-1
Кат. номер 96090512-CDNA-20UL 96090512-1VL 96090512-RNA-5UG 96090512-DNA-5UG Cell Line OriginHuman Caucasian B-cell lymphomaCell Line DescriptionThe cell line Ri-1 has been established from the peripheral blood of a 48-year-old female patient with B-cell lymphoma. This EBV-negative cell line with 14q+ abnormality was shown to be at an intermediate stage of B-cell differentiation. Ri-1 expresses surface and cytoplasmic immunoglobulin (IgM kappa), HLA-DR, CD20, CD38 and FMC7. Rearranged heavy-light chain genes and one rearranged kappa light-chain gene was detected as well as a kappa and lambda light-chain gene in germ-line configuration.ApplicationStudy of B-cell lymphoma-leukaemiaCulture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cell growth is very slow after resuscitation; large amounts of debris present.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood growth mode Suspension karyotype 92XXXX/69XXX,t(1,2)(p32,q34)t(2,8)(p11,q24)t(14,18 morphology Lymphoblastoid products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RIN-5F
Кат. номер 95090402-DNA-5UG 95090402-1VL 95090402-RNA-5UG Cell Line OriginRat islet cell tumourCell Line DescriptionRIN-5F is a secondary clone of the rat islet tumour cell line RIN-m (Sigma Catalogue number. 95071701). Cloning was performed by limited dilution in RPMI 1640, 10%FBS and 20% conditioned medium. The conditioned medium contained growth hormones and prolactin being produced by rat pituitary tumour cells GH3 (Sigma Catalogue number. 87012603). RIN-5F cells secrete insulin, produce high levels of the key APUD enzyme, L-dopa-decarboxylase and contain membrane-bound secretory granules.ApplicationStudy of pancreatic islet cell biology eg. synthesis, storage and secretion of insulinCulture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells grow in small islands and do not form a confluent monolayer.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat pancreas growth mode Adherent karyotype Not specified morphology Not specified products Insulin, L-dopa-decarboxylase receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RIN-m
Кат. номер 95071701-DNA-5UG 95071701-1VL 95071701-RNA-5UG Cell Line OriginRat islet cell tumourCell Line DescriptionEstablished from a transplantable pancreatic tumour of the islets of Langerhans. The tumour originated in an irradiated NEDH inhibited albino rat and was maintained by serial transplantation in Nedh rats. After transplantation into athymic nude mice the cell line RIN-m was established from the fourth nude mouse heterotransplant, 9 days after the tumour was removed cells were exposed to cystine-free medium resulting in the complete elimination of fibroblast-like cells. RIN-m cells secrete insulin and somatostatin. High levels of L-dopa-decarboxylase and membrane bound secretory granules were detected. RIN-5F (95090402) was established as a secondary clone from RIN-m.ApplicationStudy of biology of pancreatic islet cells, synthesis, storage and secretion of insulin and somatostatinCulture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat pancreas growth mode Adherent karyotype Not specified morphology Epithelial-like products Somatostatin, insulin receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RK-13 Cell Line from rabbit
eCl@ss: 32190102
Кат. номер 21715-1VL 21715-RNA-5UG Cell Line OriginRabbit kidneyCell Line DescriptionRK13 has been derived from kidney cells of a 5 week old rabbit. The cell line has been shown to be susceptible to pseudorabies virus, rabbitpox virus, myxomatose virus, vaccinia and EAV. Sensitivity to other viruses such as Simian adenovirus and rubella has been reported as well with the production of cytopathic effect (CPE). RK13 has been successfully treated with thiamutin for eradication of mycoplasma. The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.ApplicationVirus studiesCulture MediumEMEM(HBSS) (M5775), + 2mM L-Glutamine (G7513), + 10% FBS / FCS (F2442),.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C; monolayer is confluent after 48-56 hrs and stable for at least 21d; small syncytia in elder cultures are typical.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rabbit kidney growth mode Adherent karyotype 2n=44, stem-line = 56 (=22%) morphology Epithelial-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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RLC 27
Кат. номер 90071811-DNA-5UG 90071811-1VL 90071811-RNA-5UG Cell Line OriginRat liverCell Line DescriptionDerived from the liver of a 13 day old male rat. Mycoplasma eliminated by the original depositor with MC-210. RLC 27 is tumorigenic in nude mice.ApplicationTumourigenicity studiesCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serium (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat liver growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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RML human cell line
Кат. номер 88052106-RNA-5UG 88052106-1VL 88052106-DNA-5UG 88052106-CDNA-20UL General descriptionConsanguineous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 204
Cw: 1502
B: 5101
HLA DRA: 101
HLA DRB1: 1602
HLA DRB5: 202
HLA DRB6: 202
HLA DQA1: 5013
HLA DQB1: 301
HLA DPA1: 1
HLA DPB1: 402Serological ProfileHLA A: 2
HLA A ief: 2.4
HLA B: 51
HLA B ief: 51
Bw4/bw6: 4
HLA Dw: 22
HLA DR: 16
HLA DR51: 51
HLA DQ: 7
HLA DP: 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Native South American, Warao gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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RN 22
Кат. номер 93011414-1VL 93011414-DNA-5UG 93011414-RNA-5UG Cell Line OriginRat Schwann cell, immatureCell Line DescriptionTransplantable tumour of cervical nerve-root induced transplacentally by ethylnitrosourea in an inbred BD-IX rat.ApplicationAnalysis of Schwann cell developmentCulture MediumDMEM or Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat nerve growth mode Adherent karyotype n = 43 morphology Bipolar, stellate products S-100 protein receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RN46A
Кат. номер 12061302-DNA-5UG 12061302-RNA-5UG 12061302-1VL Cell Line OriginEmbryonic rat medullary raphe, temperature-sensitive mutant of SV40 large T-antigen, immortalised, serotonergic, neuronalCell Line DescriptionRN46A , an immortalized serotonergic neuronal cell line, was cloned by serial dilution following infection of dissociated embryonic day 13 rat medullary raphe cells with a retrovirus encoding the temperature-sensitive mutant of SV40 large T-antigen (T-ag), RN46A cells are capable of differentiating at 39 °C the non-permissive temperature. Under differentiation conditions, RN46A cells cease dividing, take on a neuronal morphology, and express enhanced levels of NSE and all three NF proteins. Differentiated RN46A cells express low-affinity nerve growth factor (NGF) receptor (p75NGFR) and are i mMunoreactive using an antibody that recognizes the carboxy-terminal 13 amino acids of all three trk proteins (pan-trk). Both i mMunoreactivities could be potentiated by treatment with brain-derived neurotrophic factor (BDNF), NGF, and adrenocorticotropic hormone, fragment 4-10 (ACTH4-10). Differentiated RN46A cells express low levels of tryptophan hydroxylase (TPH) i mMunoreactivity, which could be enhanced by treatment with ACTH4-10, BDNF, or NGF. Low levels of serotonin i mMunoreactivity are detected in differentiated RN46A cells, and this was potentiated by differentiating RN46A cells with BDNF for 8 d and 40 mM KCl for days 4-8. RN46A cells should prove useful to elucidate intracellular mechanisms that control neurofilament assembly and 5-HT expression in differentiating raphe neurons.DNA ProfileNot specifiedCulture MediumDMEM:F12 (1:1), (D8062) + L-Glutamine, (G7513) + 10% FBS / FCS, (F2442) + 0.25mg/ml Geneticin (G418). Alternatively CNS medium can be used (see Kawamoto & Barrett 1986). Differentiation can be induced as follows: cells growing at 33 °C are sub-cultured onto collagen/fibronectin matrix (100 µg/cm2 air-dried collagen I from rat tail followed by 1 µg/cm2 fibronectin). Sub-confluent cells (75%) are shifted to 39 °C. The culture medium is changed to DMEM:F12 (1:1), (D8062) + 1%(w/v) bovine serum albumin (BSA) + 1 μg/ml bovine transferrin + 5 μg/ml bovine insulin + 100 nM putrescine + 20 nM progesterone.Subculture RoutineSplit subconfluent cultures (70-80%) 1:2 to 1:5 using 0.25% trypsin/EDTA; 5% CO2; 33 °C. Suggested seeding density 2-4 x 10,000 cells/cm2. Doubling time 19hrs.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat embryo (day 13 medullary raphe) growth mode Adherent karyotype Not specified morphology Fibroblast morphology while proliferating and neuronal on differentiation receptors Not specified
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RN46A-B14
Кат. номер 12061303-DNA-5UG 12061303-RNA-5UG 12061303-1VL Cell Line OriginEmbryonic rat medullary raphe, temperature-sensitive mutant of SV40 large T-antigen, immortalised, serotonergic, neuronalCell Line DescriptionThe clonal cell line, RN46A-B14, was isolated following transfection of the gene encoding rat brain-derived neurotrophic factor (BDNF) into RN46A cells (see ECACC Catalogue number 12061302). RN46A-B14 cells synthesize and secrete biologically active BDNF in vitro and synthesize serotonin (5-HT) following partial membrane depolarization. Two weeks following RN46A-B14 cell transplantation into the adult rat cortex and hippocampus, there is a threefold increase in survival of RN46A-B14 cells compared to RN46A cells. The grafted RN46A-B14 cells i mMunohistochemically stain for BDNF and 5-HT, while RN46A cells transfected with vector only are negative for both BDNF and 5HT. In addition, RN46A-B14 cells attain more morphologically complex phenotypes, indicating enhanced neuronal differentiation. Autocrine secretion of BDNF by RN46A-B14 cells thus potentiates survival and can be used to deliver both BDNF and 5-HT in vivo.DNA ProfileNot specifiedCulture MediumDMEM:F12 (1:1), (D8062) + L-Glutamine, (G7513) + 10% FBS / FCS, (F2442) + 0.25mg/ml Geneticin (G418) + 0.1mg/ml hygromycin. Alternatively CNS medium can be used (see Kawamoto & Barrett 1986).Subculture RoutineSplit subconfluent cultures (70-80%) 1:2 to 1:5 using 0.25% trypsin/EDTA; 5% CO2; 33 °C. Suggested seeding density 2-4 x 10,000 cells/cm2. Doubling time 25hrs.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat embryo (day 13 medullary raphe) growth mode Adherent karyotype Not specified morphology Fibroblast morphology while proliferating and neuronal on differentiation receptors Not specified
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RO82-W-1
Кат. номер 92030502-DNA-5UG 92030502-1VL 92030502-CDNA-20UL 92030502-RNA-5UG Cell Line OriginHuman follicular thryoid carcinomaCell Line DescriptionEstablished from the metastases of a follicular carcinoma in a female patient. Tumorigenic in nude mice. The basal release of immuno-detectable thyroglobulin increased in response to thyroid-stimulating hormone (TSH). The cell line grows as a monolayer without the formation of follicles. Electronmicroscopy studies showed the presence of microvilli with interdigitations between neighbouring cells.ApplicationA valuable model for the study of human follicular carcinoma.Culture MediumDMEM:Ham′s F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human thyroid growth mode Adherent karyotype 2n = 60-82, 82 predominant morphology Epithelial products Thyroglobulin receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) metastasis shipped in dry ice storage temp. −196°C
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ROF-NL human cell line
Кат. номер 94071435-1VL 94071435-CDNA-20UL 94071435-RNA-5UG 94071435-DNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 03 32
B: 07 55
HLA DRB1: 1501 04
HLA DRB5: 101
HLA DQB1: 602 03Serological ProfileHLA A: 3 32
HLA C: 3 7
HLA B: 7 55
Bw4/bw6: 6
HLA DR: 2 4
HLA DR53: 53
HLA DQ: 1 3Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Not specified gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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Rolf B1.T
Кат. номер 3071601-1VL 3071601-DNA-5UG 3071601-RNA-5UG Cell Line OriginAdult rat olfactory nerve ensheathing cellsCell Line DescriptionRolf B1.T cells arose from a spontaneous immortalisation event - retaining characteristics of normal cells. The cells are dependent on serum growth factors for growth. They constitutively express glial fibrillary acidic protein, S100, the low affinity neurotrophin receptor p75 NTR, laminin, tenascin, and NCAM. These cells support the regrowth of neurites from adult rat retinal ganglion cells. They have potential value in research into mechanisms of glial support for axonal regeneration from adult mammalian central neurons and other aspects of differentiated glial cell biology. Epithelial-like or fusiform (inducible).ApplicationNeural research including axonal regeneration.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineSplit sub-confluent cultures (70-80%) 1:5 - 1:20 e.g. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat nose growth mode Adherent karyotype Normal rat chromosome complement morphology Epithelial-like products cadherin, tenascin, laminin, N-CAM, GFAP, p75 NTR receptors p75 NTR application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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RPMI 1788
Кат. номер 85112106-RNA-5UG 85112106-1VL 85112106-DNA-5UG 85112106-CDNA-20UL Cell Line OriginHuman Caucasian peripheral blood lymphocyteCell Line DescriptionDerived from the peripheral blood of an apparently normal 33 year old Caucasian male. The cells secrete IgM (lambda light chain). HLA = A2, Aw33, B7, B14.DNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9.3
TPOX: 8,9
vWA: 18,19Culture MediumRPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-4x100,000 cells/ml; 5% CO2; 37°C. Maximum cell density 1-2x1,000,000 cells/ml.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood growth mode Suspension karyotype 2n = 46, modal no. 45 morphology Lymphoblast products Immunoglobulin M (IgM) (Lambda light chain) receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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RPMI 1846
Кат. номер 93120834-RNA-5UG 93120834-1VL 93120834-DNA-5UG Cell Line OriginHamster Syrian melanotic melanomaCell Line DescriptionDerived from a spontaneous malignant melanoma in an aged golden Syrian hamster. The cells have the ability to produce melanin in vitro. The TD50 for new-born hamsters is 68 cells.ApplicationTumourigenicity studiesCulture MediumMcCoy′s 5a + 2mM Glutamine + 10-20% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 i.e. seeding at 1.5x1,000,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source hamster skin growth mode Adherent karyotype 2n = 44 morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RPMI 2650
Кат. номер 88031602-CDNA-20UL 88031602-DNA-5UG 88031602-1VL 88031602-RNA-5UG Cell Line OriginHuman nasal septum quasidiploid tumourCell Line DescriptionFrom the pleural effusion of an extensive malignant tumour of the nasal septum, diagnosed as anaplastic squamous cell carcinoma. Nearly normal karyotype, stability of chromosome number and probable origin from malignant cells make the cell line unique amongst permanent human cell lines. Cells are very small and grow in clusters fusing to form thick layers.ApplicationTumourigenicity studiesDNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,11
D13S317: 11,12
D16S539: 11,12
D5S818: 12,13
D7S820: 8,11
THO1: 6,8
TPOX: 8
vWA: 16,18Culture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human nose growth mode Adherent karyotype 2n = 46 morphology Epithelial products Mucoid material receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RPMI 6666
Кат. номер 87081807-DNA-5UG 87081807-RNA-5UG 87081807-1VL 87081807-CDNA-20UL Cell Line OriginHuman Caucasian lymphoblast, Hodgkins diseaseCell Line DescriptionDerived from a 29 year old male Caucasian with Hodgkin′s disease. One of a large series of permanent lines from leukocytes in the peripheral blood of donors with and without malignancies. Has cell-associated EBV and produces immunoglobulins. This cell line was initially thought to have been derived from Hodgkin lymphoma, but is now known to be an EBV-positive B-lymphoblastoid cell line.ApplicationTumourigenicity studiesCulture MediumRPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood growth mode Suspension karyotype 2n = 46 morphology Lymphoblast products Immunoglobulin receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RPMI 7666
Кат. номер 87012703-CDNA-20UL 87012703-RNA-5UG 87012703-1VL 87012703-DNA-5UG Cell Line OriginHuman Caucasian lymphoblastCell Line DescriptionInitiated from the leukocytes obtained from a 32 year old Caucasian male having no known malignancy. EBV particles are present. The cells produce immunoglobulins in vitro.ApplicationTumourigenicity studiesDNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 9,11
D5S818: 11,12
D7S820: 8,11
THO1: 7,9.3
TPOX: 8,11
vWA: 14,16Culture MediumRPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood growth mode Suspension karyotype 2n = 46 morphology Lymphoblast products Immunoglobulin receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RPMI 7932 cell line 94072246-1VL
Cell Line OriginHuman pleural effusion from a patient with malignant melanomaCell Line DescriptionDerived from the pleural effusion of a 36 year old female with malignant melanoma. Cell line produces plasminogen activator. This cell line was found to be indistinguishable from cell lines COLO 699N & COLO 738 by STR PCR DNA profiling. At ECACC the STR PCR profile of all stocks of this cell line match the profile of the original source material received at ECACC. However, it is not clear if the cell lines COLO 699N, RPMI 7932 & COLO 738 were originally derived from the same individual as the profiles suggest. The cell lines COLO 699N, RPMI 7932 & COLO 738 were received from the same depositor. If we are able to obtain clarification of the relationship between these cell lines this information will be updated.ApplicationTumourigenicity studiesCulture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lung (effusion) Quality Level 100, growth mode Adherent karyotype Not specified morphology Epithelial-like products Plasminogen activator receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RPMI 8226
Кат. номер 87012702-RNA-5UG 87012702-1VL 87012702-DNA-5UG 87012702-CDNA-20UL Cell Line OriginHuman myelomaCell Line DescriptionDerived from the peripheral blood of a 61 year old male with multiple myeloma. The cells produce and secrete Ig lambda light chain.ApplicationRPMI 8226 has been used:- to grow human myeloma cell lines
- to evaluate the effect of 1-aryl-2-methylthioimidazolines (IIV) on the viability of RPMI 8226 cells
- to measure cytokine production by bone marrow stromal cells (BMSCs) after contact with the RPMI 8226 cell line
DNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18Culture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. After resuscitation cell debris will be present in culture. This will decrease with subsequent subculture.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood growth mode Suspension karyotype Not specified morphology Not specified products Immunoglobulin G (Ig) lambda light chain receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RPMI 8866 Cell Line human
Кат. номер 95041316-RNA-5UG 95041316-1VL 95041316-CDNA-20UL 95041316-DNA-5UG Cell Line OriginHuman B lymphocyteCell Line DescriptionRPMI 8866 has been established from the peripheral blood of a 51-year-old American woman with chronic myelogenous leukaemia in May 1966. The B lymphoid cell line expresses a relatively high amount of calcitonin receptors, membrane bound Ig and soluble IgE-binding factor (Fc epsilon RII/CD23).ApplicationReceptor studies, binding assays, hormone studies.DNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10,14
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 9.3
TPOX: 8,11
vWA: 14,16Culture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineTo keep the cells in exponential growth maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. If starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 200ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 10% serum. Incubate flask at 37 C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth, this can take up to 7 days.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood growth mode Suspension karyotype Not specified morphology Lymphoblastoid products Not specified receptors Immunoglobulin E (IgE) binding factor, Calcitonin application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RR-CHOKI
Кат. номер 92052129-RNA-5UG 92052129-1VL 92052129-DNA-5UG Cell Line OriginHamster Chinese ovaryCell Line DescriptionDerived from the parent line CHO-KI, resistant to ionising radiation and UV irradiation.ApplicationRadiation sensitivity and mutation studiesCulture MediumHam′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cell/cm2 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source hamster ovary growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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RR1022
Кат. номер 90102526-RNA-5UG 90102526-DNA-5UG 90102526-1VL Cell Line OriginRat Amsterdam Schmidt-Ruppin sarcomaCell Line DescriptionDerived from a Rous sarcoma virus (Schmidt-Ruppin strain) induced tumour in an inbred Amsterdam rat. The cells are highly tumorigenic in new-born rats and also chickens. There is some evidence that the cell line is latently infected with the virus.ApplicationTumourigenicity and virus studies: Rous sarcoma virusCulture MediumMedium 199 (HBSS) + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat unknown/unspecified growth mode Adherent karyotype 2n = 42 morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RSA-ND human cell line
Кат. номер 94022546-RNA-5UG 94022546-1VL 94022546-DNA-5UG 94022546-CDNA-20UL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 0201 3401
B: 1513 3802
HLA DRB1: 15
HLA DQB1: 05Serological ProfileHLA A: 2 34
HLA C: 7
HLA B: 77 38
Bw4/bw6: 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Filipino gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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RSH human cell line
Кат. номер 88052021-RNA-5UG 88052021-DNA-5UG 88052021-CDNA-20UL 88052021-1VL General descriptionConsanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 6802 3001
Cw: 1701
B: 4201
HLA DRA: 102
HLA DRB1: 302
HLA DRB3: 101
HLA DQAP: 4.2
HLA DQA1: 401
HLA DQB1: 402
HLA DPA1: 0301 0202
HLA DPB1: 0101 0402Serological ProfileHLA A: 68 30
HLA A ief: 68.1 30.3
HLA C: 2
HLA B: 42
HLA B ief: 42
Bw4/bw6: 6
HLA Dw: RSH
HLA DR: 18
HLA Dw2: 24
HLA DR52: 52
HLA DQ: 4
HLA DP: 1 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity South African Zulu gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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RT 1
Кат. номер 94011416-1VL 94011416-RNA-5UG 94011416-DNA-5UG Cell Line OriginRat Lewis fibrosarcomaCell Line DescriptionDerived during co-culture of rat lewis embryonic fibroblasts with chicken sarcoma cells originally obtained from a tumour induced in vivo by RSV-33. The cells contain the viral genome but do not express virus specific RNA. The cells are tumourigenic in rats.Culture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat unknown/unspecified growth mode Adherent karyotype Modal no. 40 morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RT112/84
Кат. номер 85061106-1VL 85061106-RNA-5UG 85061106-CDNA-20UL Cell Line OriginHuman bladder carcinoma epithelialCell Line DescriptionA slow growing tumour which does not form colonies in soft agar. Tumorigenic in nude mice, RT1112/84 cells possess a low plating efficiency requiring 400 cells/dish to form any colonies.ApplicationRT112/84 cancer cell line has been used to study the cytotoxic effect of lipoic acid and calcium hydroxycitrate concentrations on RT112/84 cells.
Tumourigenicity studiesDNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13,14
D16S539: 11,13
D5S818: 10,13
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 14,17Culture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Subculture weekly - cells are difficult to trypsinise, and should be kept sub-confluent.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human bladder growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RT4
Кат. номер 91091914-RNA-5UG 91091914-CDNA-20UL 91091914-1VL 91091914-DNA-5UG Cell Line OriginHuman Caucasian bladder transitional-cell carcinomaCell Line DescriptionDerived from explants of a recurring papillary tumour of the bladder.ApplicationRT4 cell line has been used to study the effects of uropathogenic Escherichia coli on neutrophil adhesion via neutrophil adhesion assay.
Tumourigenicity studiesDNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8
D16S539: 9
D5S818: 11,12
D7S820: 9,12
THO1: 9,9.3
TPOX: 8,11
vWA: 14,17Culture MediumMcCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human bladder growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. 196°C
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RT4-D6P2T
Кат. номер 93011415-1VL 93011415-RNA-5UG 93011415-DNA-5UG Cell Line OriginRat Schwann cell, immatureCell Line DescriptionDerived from the ethylnitrosourea-induced tumour line D6 of the rat peripheral nervous system. Cells will de-differentiate after 10 passages. Read references prior to use.ApplicationSchwann cell gene regulation and developmentCulture MediumDMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat nerve growth mode Adherent karyotype Not specified morphology Bipolar products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RT4/31
Кат. номер 86082102-CDNA-20UL 86082102-RNA-5UG 86082102-DNA-5UG 86082102-1VL Cell Line OriginHuman bladder transitional cell papillomaCell Line DescriptionDerivative of RT4 which was derived from a transitional cell papilloma of a human bladder.Culture MediumMcCoys 5a + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS)Subculture RoutineSplit confluent cultures 1:3-1:6 ie seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/ EDTA ; 5%CO2; 37°COther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human bladder growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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RTG-2
Кат. номер 90102529-DNA-5UG 90102529-RNA-5UG 90102529-1VL Cell Line OriginTrout Rainbow gonad tissueCell Line DescriptionFrom pooled male/female gonad tissue of yearling rainbow trout (Oncorhynchus mykiss). First cell line to be established from cold-blooded vertebrates. 8-fold difference in growth rate in temperature range 4-24°C. Susceptible to infectious pancreatic necrosis virus, Egtved virus and arboviruses (EEE, VEE).ApplicationVirus studies: Inf. pacr. necrotic virus, Egtved virus, arboviruses (EEE, VEE)Culture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutinePassage using 0.25% trypsin below 24°C. Cells may be seeded as few as 3x10,000 cells/ml 5% CO2; 24°C. Fish cell lines detach easily during transit if the culture is very younOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source fish ovary growth mode Adherent karyotype 2n = 60 morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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S1814.PB5
Кат. номер 85011402-DNA-5UG 85011402-1VL 85011402-CDNA-20UL 85011402-RNA-5UG Cell Line OriginHuman embryo fibroblastCell Line DescriptionHuman embryo fibroblast cell line.DNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 11
D5S818: 13
D7S820: 10,11
THO1: 7,9
TPOX: 9,11
vWA: 15Culture MediumMcCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells should also grow in DMEM or RPMI 1640. This cell line also grow well in HAT.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:10 i.e. seeding 3-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human embryo growth mode Adherent karyotype Diploid morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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S2.1
Кат. номер 92040116-1VL 92040116-RNA-5UG 92040116-DNA-5UG Cell Line OriginPig L231 x boar spleen (d/d haplotype)Cell Line DescriptionThe antibody is directed against the ST cell antigen as determined in virus enzyme linked cell immunoassays (VELCIA). The antibody does not cross-react with mouse 3T3 cells.ImmunogenSwine testis cell culture supernatant containing transmissible gastro-enteritis virus (strain Nouzilly 188.SG)ApplicationVELICA, ELISACulture MediumRPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 2-10x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Pig x Pig Hybridoma ImmunoDon Boar spleen (d/d haplotype) Myeloma L231 (lymphoblastoid) Reactivity Anti-Swine Testis (ST) cell antigen application(s) cell culture | mammalian: suitable isotype IgA shipped in dry ice storage temp. −196°C
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S2.2
Кат. номер 92040117-DNA-5UG 92040117-1VL 92040117-RNA-5UG Cell Line OriginPig L231 x boar spleen (d/d haplotype)Cell Line DescriptionThe antibody is directed against swine testis cell antigen as determined in virus enzyme linked cell immunoassays (VELCIA). The antibody does not cross-react with mouse 3T3 cells.ImmunogenSwine testis cell culture supernatant containing transmissible gastro-enteritis virus (strain Nouzilly 188.SG)ApplicationVELICA, ELISACulture MediumRPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 2-10x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Pig x Pig Hybridoma ImmunoDon Boar spleen (d/d haplotype) Myeloma L231 (lymphoblastoid) Reactivity Anti-Swine Testis (ST) cell antigen application(s) cell culture | mammalian: suitable isotype IgA shipped in dry ice storage temp. −196°C
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SA human cell line
Кат. номер 88052001-DNA-5UG 88052001-1VL 88052001-CDNA-20UL 88052001-RNA-5UG General descriptionHomozygous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 2402
Cw: 702
B: 702
HLA DRB1: 01
HLA DQB1: 05
HLA DPB1: 402Serological ProfileHLA A: 24
HLA A ief: 24.1
HLA C: 7
HLA B: 7
HLA B ief: 7.2
Bw4/bw6: 6
HLA Dw: 1
HLA DR: 1
HLA DQ: 1
HLA DP: 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Japanese gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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SAF-1
Кат. номер 122301-RNA-5UG 122301-DNA-5UG 122301-1VL Cell Line OriginGilt head seabream caudal finCell Line DescriptionSAF-1 has been developed from the fin tissues of an adult gilt-head seabream (sparius aurata) without immortalising treatments. SAF-1 supports the replication of several common fish viruses such as lymphocystis disease virus (LDV), infectious haematopoietic necrosis virus (IHNV) and viral haemorrhagic septicaemia virus (VHSV). SAF-1 has a higher susceptibility to rhabdoviruses, especially UHNV. In the SAF-1 line, neither chromosome changes or differences from passage 50-70 have been detected, suggesting a normal karyotype and stable cell line. The cell line may be useful in isolating and studying undetected latent and potential lytic viruses infecting gilt-head seabream and other related sparid species. In addition to its proven applications, SAF-1 could be used as an in vitro system to address multiple questions related to the genetic engineering of this species, such as evaluating the efficiency of the promotors in transgenic constructions, testing whether this species is susceptible to viral vectors available in fish or whether heterologous transposable elements can be mobilised for insertional mutagenesis. This cell line was mycoplasma eradicated at ECACC.ApplicationGenetic engineering, virus susceptibility, fish physiology.Culture MediumL15 (L5520), + 4mM L-Glutamine (G7513), + 15% FBS / FCS (F2442),Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 -1.6 ie. seeding at 2-4 x10,000 cells/cm2 using 0.25% trypsin/EDTA; 25°C; no CO2.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source fish fin growth mode Adherent karyotype Not specified morphology Fibroblast-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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