Mammalian Cell Lines
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NCTC 4206
Кат. номер 91010303-1VL 91010303-DNA-5UG 91010303-RNA-5UG Cell Line OriginHamster Chinese peritoneumCell Line DescriptionA chemically defined medium derivative of B14FAF28-G3. Since it was established, NCTC cells have been employed in nutritional studies involving 6 different NCTC chemically defined serum-free media.ApplicationNutritional studiesCulture MediumNCTC 135 100% + 2mM Glutamine.Subculture RoutineSub-cultures are prepared by scraping; 5% CO2; 37°C. Media change three times a week. Freeze in 9% glycerol/91% FBS.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source hamster peritoneum growth mode Adherent karyotype 2n = 22; Modal no. 23 morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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NCTC clone 1469
Кат. номер 88111403-1VL 88111403-DNA-5UG 88111403-RNA-5UG Cell Line OriginMouse C3H/An liverCell Line DescriptionDerived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).ApplicationCell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)Culture MediumNCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO2; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearanOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse liver growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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NCTC clone 929
Кат. номер 88102702-DNA-5UG 88102702-RNA-5UG 88102702-1VL Cell Line OriginMouse C3H/An connective tissueCell Line DescriptionFrom parental strain L which was derived from normal subcutaneous areolar and adipose tissue of 100 day old male C3H/An. Used in development of replicate culture techniques, chemically defined media, fluid suspension culture methods and virus assays. Susceptible to pseudorabies virus and vesicular stomatitis (Indiana strain) virus.ApplicationReplication, biochemical, neoplastic transformation, nutritional, metabolic enzyme, morphological, chromosome, immunologic, radiation, toxicologic and virus studiesCulture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Freeze in 10% glycerol/90% FBS. After resuscitation cells may take up to 4 days to establish growth.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse connective tissue growth mode Adherent karyotype 2n = 40 morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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ND-C
Кат. номер 92090913-DNA-5UG 92090913-1VL 92090913-RNA-5UG Cell Line OriginMouse neuroblastoma x Rat neurone hybridCell Line DescriptionND-C hybrid cells were derived after fusion of primary neonatal-rat dorsal-root-gauglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300) derived azaguanine resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. The cells express rat Thy 1.1 marker and the sensory neuropeptide substance P. The cell line has been mycoplasma eradicated at ECACC.ApplicationStudy of neuronal differentiation, cell adhesion and expression of neuronal proteins.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3 x 10,000 cells/cm2; aspirate or gently tap cells loose, use of trypsin not necessary; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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ND15
Кат. номер 92090907-1VL 92090907-RNA-5UG 92090907-DNA-5UG Cell Line OriginMouse neuroblastoma x Rat neurone hybridCell Line DescriptionThe hybrid cell line ND15 was derived after fusion of primary neonatal-rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)- derived azaguanine-resistant line. HAT-sensitive hybrids were selected and cloned by limiting dilution. The cells express all isoforms of N-CAM but no embryonic form. Thy 1.1 marker, P31 and PrPc can be detected as well as a weaker expression of F3 and Thy-3. When differentiated in serum free media an increased level of Thy-2 can be detected.ApplicationStudy of neuronal differentiation, cell adhesion and expression of neuronal proteinsCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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ND27
Кат. номер 92090912-DNA-5UG 92090912-RNA-5UG 92090912-1VL Cell Line OriginMouse neuroblastoma x Rat neurone hybridCell Line DescriptionMouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion. The cell line has been mycoplasma eradicated at ECACC.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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ND3
Кат. номер 92090901-DNA-5UG 92090901-1VL 92090901-RNA-5UG Cell Line OriginMouse neuroblastoma x Rat neurone hybridCell Line DescriptionND3 hybrid cells were derived after fusion of primary neonatal-rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)-derived azaguanine-resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. The cells express all isoforms of N-CAM but no embryonic form. Thy 1.1 marker, P31 and PrPc can be detected as well as a weaker expression of F3 and Thy-3.ApplicationStudy of neuronal differentiation, cell adhesion and expression of sensory neuron specific markerCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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ND7/23 Cell Line
Кат. номер 92090903-1VL 92090903-RNA-5UG 92090903-CDNA-20UL Cell Line OriginMouse neuroblastoma x Rat neurone hybridCell Line DescriptionMouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion.ApplicationSubculture: Split at 70-80% confluency, approx. 1:3 to 1:6, seed at 1-3x104 viable cells/cm2 at 37°C and 5% CO2. Dissociate cells by aspiration or gentle tapping. Cells are lightly adherent, no trypsinization is necessary.
Recommended culture medium: DMEM + 2 mM L-Glutamine + 10% FBS
Mouse neuroblastoma (N18 tg 2) and rat dorsal root glanglia produced by PEG-mediated fusion.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. 196°C
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ND8/34 Cell Line from mouse
Кат. номер 92090904-RNA-5UG 92090904-DNA-5UG 92090904-1VL Cell Line OriginMouse neuroblastoma x Rat neurone hybridCell Line DescriptionThe hybrid ND8/34 is a subclone of ND8 which was derived after fusion of primary neonatal rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)-derived azaguanine-resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. ND8/34 are electrically excitable to the chemical activator of nociceptive sensory neurons, bradykinin.ApplicationStudy of neuronal differentiation.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve description Mouse neuroblastoma x Rat neurone hybrid growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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Neopu
Кат. номер 90070561-RNA-5UG 90070561-DNA-5UG 90070561-1VL Cell Line OriginDrosophila embryoCell Line DescriptionSupports the growth of rhabdovirus and picornavirus. Developed from the embryonic cells of Drosophila melanogaster.ApplicationVirus studies: rhabdovirus, picornavirusCulture MediumSchneider′s Drosophila medium + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml. Cells may semi-adhere to the tissue culture flask, shake gently to dislodge; 25°C; No CO2.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source insect embryo growth mode Semi-adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Neuro 2a Cell Line from mouse
Кат. номер 89121404-1VL 89121404-RNA-5UG Cell Line OriginMouse Albino neuroblastomaCell Line DescriptionDerived from a spontaneous tumour in an albino strain A mouse. Cells produce microtubular protein which is believed to play a role in the contractile system giving axoplasmic flow in nerve cells.ApplicationNeuro 2a cell line has been used as a model to study the potential toxicological effects of copper oxide nanoparticles accumulation in the brain. It has also been used to determine the sensitivity of the cell line to saxitoxin.
Tumourigenicity studies, microtubular protein synthesis, vinblastine precipitation, GTP binding kineticsCulture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 4x10,000 cells cm2; 5% CO2; 37°C. After resuscitation cells can take up to 6 days before a monolayer forms. These cells only adhere to the culture flask very lightly, care should be taken when subculturing. There is no need to wash the cells with PBS or trypsin, merely pour off half of the spent medium carefully, give the flask a gentle knock and resuspend the cells in fresh medium using a pipette to gently disperse any clumps of cells to finally reseed at 1:2. On arrival, growing cultures should be spun out and re-seeded in fresh media.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve description Mouse Albino neuroblastoma growth mode Adherent karyotype 2n = 40 morphology Neuronal/amoeboid-like products Microtubular protein receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NFS-25 C-3
Кат. номер 93090713-RNA-5UG 93090713-1VL 93090713-DNA-5UG Cell Line OriginMouse pre-B lymphoblastCell Line DescriptionDerived from a spontaneous lymphoma in an NFS.C58v-1 mouse. These pre-B cells are negative for surface Ig, Ia and ThB.Culture MediumDMEM + 10% NCTC 109 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 1mM Oxaloacetic acid + 50μM 2-Mercaptoethanol (2ME) + 2μg/ml Transferrin + 0.2IU/ml Insulin + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cellOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse blood growth mode Suspension karyotype Not specified morphology Lymphoblast products Not specified receptors Ly-17, Lyb-2, Ly-1, Ly-5 (B220) application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NFS-5 C1
Кат. номер 88041904-RNA-5UG 88041904-DNA-5UG 88041904-1VL Cell Line OriginMouse pre-B lymphoblastCell Line DescriptionEstablished from a lymphoma produced in an NFS/N mouse by injecting CaS-25M ecotropic MuLV. Cells show markers of large pre-B lymphoblasts and spontaneously produce ecotropic and mink cell focus-forming viruses.ApplicationVirus studies: MuLV, ecotropic and focus-forming virusesCulture MediumDMEM + 2mM Glutamine + 1mM Oxaloacetic acid + 0.2U/ml Bovine Insulin + 2ug/ml Transferrin + 50μM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse blood growth mode Suspension karyotype Not specified morphology Lymphoblast products Ecotropic and mink cell focus-forming viruses receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NFS-70 C10
Кат. номер 88041905-RNA-5UG 88041905-1VL 88041905-DNA-5UG Cell Line OriginMouse Pro-B lymphoblastCell Line DescriptionEstablished from a lymphoma produced in an NFS/N mouse by injecting CaS-NS-7 ecotropic MuLV. Cells show pre-B cell markers and appear to be at a very early stage of B-cell differentiation.ApplicationDifferentiation and virus studies: MuLVCulture MediumDMEM + 2mM Glutamine + 1mM Oxaloacetic acid + 0.2U/ml Bovine Insulin + 2ug/ml Transferrin + 50μM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse blood growth mode Suspension karyotype Not specified morphology Lymphoblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NG108-15
Кат. номер 88112302-1VL 88112302-RNA-5UG 88112302-DNA-5UG Cell Line OriginMouse neuroblastoma x Rat glioma hybridCell Line DescriptionNeuroblastoma x glioma hybrid was formed by Sendai virus-induced fusion of the mouse neuroblastoma clone N18TG-2 and the rat glioma clone C6 BV-1.ApplicationHybridisation, fusion partner and virus studies: Sendai virusCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 seeding at 2x100,000 cells/cm2 . Cells adhere lightly, detach by gently knocking the flask do not use trypsin. 5% CO2; 37°C. The cells do produce large amounts of CO2, media changes may be necessary.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NG115-401L
Кат. номер 87032003-RNA-5UG 87032003-1VL 87032003-DNA-5UG Cell Line OriginMouse neuroblastoma x Rat glioma hybridCell Line DescriptionA somatic hybrid derived by the hybridisation of the clonal mouse neuroblastoma N115TG-2 and the clonal rat glioma C6BV-1. The cells produce significant amounts of IR-substance P-like material.ApplicationHybridisation and fusion partner studiesCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma hybrid growth mode Adherent karyotype Not specified morphology Glial products IR-substance P-like material receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NH15-CA2
Кат. номер 8062525-1VL 8062525-DNA-5UG 8062525-RNA-5UG Cell Line OriginMouse neuroblastoma x rat glioma hybridCell Line DescriptionNH15-CA2 is a hybrid cell line formed by the fusion of N115-BU-8 (a bromodeoxyuridine-resistant mutant of N1E-115 (Sigma Catalogue number 08062519) and 108CC15 (Sigma Catalogue number 08062516) (itself a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid line). This “hybrid-hybrid” line contains both cholinergic and adrenergic enzyme activities.Applicationused as a neuronal modelCulture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 daysSubculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Glioma Hybrid brain growth mode Adherent karyotype Not specified morphology Neuronal products Acetylcholine and noradrenaline receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NH17
Кат. номер 92111101-CDNA-20UL 92111101-RNA-5UG 92111101-1VL 92111101-DNA-5UG Cell Line OriginHuman T cell lymphoblastic leukaemiaCell Line DescriptionSubclone of MOLT-4 (Sigma Catalogue number. 85011413) and is a high expresser of the cortical thymocyte differentiation antigen HTA1 and can be used for the production of CD1DmRNA.ApplicationProduction of CD1DmRNACulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells should not remain in culture for long periods otherwise expression of CD1 is lost.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood growth mode Suspension karyotype Not specified morphology Lymphoblast products High expresser of surface HTA1 receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NI 1-_-364/3B3-14
Кат. номер 92030611-1VL 92030611-RNA-5UG 92030611-DNA-5UG Cell Line OriginNot specifiedCell Line DescriptionThis is a weakly neutralising antibody specific for human IL-1, both natural and recombinant.ImmunogenHuman recombinant interleukin-1ApplicationImmunoassaysCulture MediumRPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS0 Reactivity Anti-interleukin-1, human application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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NI 1-_-364/8C4-8
Кат. номер 92030612-DNA-5UG 92030612-1VL 92030612-RNA-5UG Cell Line OriginNot specifiedCell Line DescriptionThe antibody is specific for human IL-1, both natural and recombinant.ImmunogenHuman recombinant interleukin-1ApplicationImmunoassaysCulture MediumRPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS0 Reactivity Anti-interleukin-1, human application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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NI 3 416/6C6-6
Кат. номер 92030615-DNA-5UG 92030615-1VL 92030615-RNA-5UG Cell Line OriginNot specifiedCell Line DescriptionThe antibody binds to at least 2 different forms of recombinant human IL-3.ImmunogenRecombinant human interleukin-3ApplicationImmunoassaysCulture MediumRPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS0 Reactivity Anti-interleukin-3, human application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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NI 3 416/7B5-5
Кат. номер 92030616-RNA-5UG 92030616-1VL 92030616-DNA-5UG Cell Line OriginNot specifiedCell Line DescriptionThe antibody binds to at least 2 different forms of recombinant human IL-3.ImmunogenRecombinant human interleukin-3ApplicationImmunoassaysCulture MediumRPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS0 Reactivity Anti-interleukin-3, human application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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NI 4 426/1A6-10
Кат. номер 92030617-1VL 92030617-DNA-5UG 92030617-RNA-5UG Cell Line OriginMouse NS0 x Mouse BALB/c spleenCell Line DescriptionThis neutralising antibody binds to all forms of human IL-4 tested, natural and recombinant.ImmunogenRecombinant human interleukin-4ApplicationImmunoassaysCulture MediumRPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS0 Reactivity Anti-interleukin-4, human application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice
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NI 4 426/7A3-3
Кат. номер 92030618-DNA-5UG 92030618-RNA-5UG 92030618-1VL Cell Line OriginMouse NS0 x Mouse BALB/c spleenCell Line DescriptionThis neutralising antibody binds to all forms of human IL-4 tested, natural and recombinant.ImmunogenRecombinant human interleukin-4ApplicationImmunoassaysCulture MediumRPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS0 Reactivity Anti-interleukin-4, human application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice
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NI 4 426/8B1-7
Кат. номер 92030619-DNA-5UG 92030619-1VL 92030619-RNA-5UG Cell Line OriginMouse NS0 x Mouse BALB/c spleenCell Line DescriptionThis neutralising antibody is specific for human IL-4 both natural and recombinant.ImmunogenRecombinant human interleukin-4ApplicationImmunoassaysCulture MediumRPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS0 Reactivity Anti-interleukin-4, human application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice
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NI G 386/3B6-12
Кат. номер 92030614-RNA-5UG 92030614-DNA-5UG 92030614-1VL Cell Line OriginNot specifiedCell Line DescriptionThe antibody is specific for human granulocyte colony stimulating factor, both recombinant and natural forms.ImmunogenRecombinant human granulocyte colony stimulating factor (G-CSF)ApplicationImmunoblot, immunoassaysCulture MediumRPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS0 Reactivity Anti-granulocyte colony stimulating factor (G-CSF), human application(s) cell culture | mammalian: suitable isotype IgG2a shipped in dry ice storage temp. −196°C
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NI G 386/3D1-16
Кат. номер 92030613-DNA-5UG 92030613-RNA-5UG 92030613-1VL Cell Line OriginNot specifiedCell Line DescriptionThis neutralising antibody is specific for human granulocyte colony stimulating factor (G-CSF), both recombinant and natural.ImmunogenHuman recombinant granulocyte colony stimulating factor (G-CSF)ApplicationImmunoassaysCulture MediumRPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS0 Reactivity Anti-granulocyte colony stimulating factor (G-CSF), human application(s) cell culture | mammalian: suitable isotype IgG2a shipped in dry ice storage temp. −196°C
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NI GM 465/7A6-37
Кат. номер 92030620-RNA-5UG 92030620-DNA-5UG 92030620-1VL Cell Line OriginMouse NS0 x Mouse BALB/c spleenCell Line DescriptionThis neutralising antibody is specific for GM-CSF.ImmunogenHuman recombinant granulocyte macrophage colony stimulating factorCulture MediumRPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS0 Reactivity Anti-GM-CSF, human application(s) cell culture | mammalian: suitable isotype IgG2a shipped in dry ice storage temp. −196°C
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NIH 3T3 Cell Line murine
Кат. номер 93061524-CDNA-20UL 93061524-RNA-5UG 93061524-1VL Cell Line OriginMouse Swiss NIH embryoCell Line DescriptionEstablished from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.ApplicationDNA transfection studies
NIH 3T3 Cell Line has been used to:- study calcium-mediated actin reset (CaAR) in response to physiological changes
- study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation
- determine the viral titer of the retroviral construct -ST40hTNF
Culture MediumDMEM + 2mM Glutamine + 10% Calf Serum (CS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse embryo description Embryonic fibroblast growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. 196°C
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NIH-3T3 4-2
Кат. номер 86041101-RNA-5UG 86041101-1VL 86041101-DNA-5UG General descriptionProduct has lost contact inhibitionCell Line OriginMouse fibroblastCell Line DescriptionA mouse fibroblast cell line which has an integrated mutant Moloney MuLV. The cell line can be used to generate helper-free defective retroviruses.ApplicationVirus studies: Moloney MuLV, retrovirusesCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse skin growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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NIH-3T3D4
Кат. номер 85111801-DNA-5UG 85111801-RNA-5UG 85111801-1VL General descriptionProduct has lost contact inhibitionCell Line OriginMouse Swiss NIH embryoCell Line DescriptionDerived from the NIH-3T3 cell line. No longer exhibit contact inhibition.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2x10,000 to 2x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse embryo growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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NMuMG
Кат. номер 94081121-1VL 94081121-DNA-5UG 94081121-RNA-5UG Cell Line OriginMouse mammary gland, normal epithelialCell Line DescriptionInitiated from the mammary gland tissue of an adult NAMRU mouse and one of a series of epithelial cell lines derived from normal glandular tissue of mice. The cells exhibit many ultrastructural features of secretory epithelium as well as desmosomes or intermediate junctions, microvilli and tonofibrils. Hemicytes or domes develop when dense monolayers are maintained for several weeks without sub-cultivation. The cells produce benign cystadenomas when inoculated into isogenic mice and are negative for type C oncornaviruses.ApplicationNMuMG has been used to compare the actions of insulin like growth factor binding protein 5 (IGFBP-5) with transforming growth factor β 1 (TGFβ1) on epithelial cells. It has also been used to measure the influence of phorbol 12-myristate 13-acetate (PMA) and estradiol on bone morphogenetic protein receptor type 2 (BMPR2) gene expression.Culture MediumDMEM + 2mM Glutamine + 10ug/ml Insulin + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse breast growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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NON, L human cell line
Кат. номер 94082292-1VL 94082292-DNA-5UG 94082292-CDNA-20UL 94082292-RNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileHLA DRB1: 0406 0803
HLA DRB4: 101
HLA DQAP: 1.4 3.1
HLA DQA1: 0103 03
HLA DQB1: 0601 0302
HLA DPB1: 02012 0501Serological ProfileHLA A: 1 10
HLA C: 1
HLA B: 51 55
Bw4/bw6: 4 6Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Australian Aborigine gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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NORIg 7.16.2
Кат. номер 11060818-1VL 11060818-DNA-5UG 11060818-RNA-5UG Cell Line OriginMonoclonal antibody rat IgG2bCell Line DescriptionMouse hybridoma against rat IgG2b heavy chain. Cambridge Enterprise Reference number AB71:www.enterprise.cam.ac.uk.ImmunogenRat IgGCulture MediumIDMEM, (I3390) + 2 mM L-Glutamine, (G7513) + 5% FBS / FCS, (F2442). Where the cells are grown under static conditions, 0.02% EDTA, Trypsin, (T4424) or mechanical agitation maybe required to dislodge them from the plastic.Subculture RoutineMaintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon Balb/c mouse spleen Myeloma NS0 mouse myeloma Reactivity Rat IgG2b heavy chain application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice
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NP cl.120
Кат. номер 95040312-1VL 95040312-RNA-5UG 95040312-DNA-5UG Cell Line OriginMouse Sp2/0 x Mouse BALB/c spleenCell Line DescriptionNP cl.120 monoclonal antibody is one of a range of well characterised measles virus antibodies used to measure synthesis and topological accumulation of measles virus antigen during the lytic cycle. This anti-nucleoprotein antibody is negative for haemagglutinin and hemolysin inhibition as well as neutralisation assays and gives immunofluorescence only on fixed tissue.ImmunogenMeasles virus (strain Hall)ApplicationDiagnosis, study of measles virus infectionCulture MediumRPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 1-5x100,000 cells/ml; 5% CO2; 37°COther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Ag-14 Reactivity Anti-Measles Virus Nucleoprotein application(s) cell culture | mammalian: suitable isotype IgG2a shipped in dry ice storage temp. −196°C
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NP cl.25
Кат. номер 95051114-DNA-5UG 95051114-RNA-5UG 95051114-1VL Cell Line OriginMouse Sp2/0 x Mouse BALB/c spleenCell Line DescriptionNP cl.25 monoclonal antibody was produced in vitro by fusing mouse myeloma cells with spleen cells derived from BALB/c mice immunised with purified measles virus. NP cl.25 is negative for haemagglutination and hemolysin inhibition as well as neutralisation assays and gives immunofluorescence only on fixed tissue. It has been used for measuring synthesis and topological accumulation of measles virus antigen during the lytic cycle.ImmunogenMeasles virus (strain Hall)ApplicationDiagnosis, study of measles virus infectionCulture MediumRPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 1-5x100,000 cells/ml; 5% CO2; 37°COther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Ag-14 Reactivity Anti-Measles Virus Nucleoprotein application(s) cell culture | mammalian: suitable isotype IgG2a shipped in dry ice storage temp. −196°C
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NP369 human cell line
Кат. номер 94082255-DNA-5UG 94082255-1VL 94082255-CDNA-20UL 94082255-RNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 02 11
B: 58
HLA DRB1: 301
HLA DRB3: 202
HLA DQAP: 4.1
HLA DQA1: 501
HLA DQB1: 201
HLA DPB1: 0401 0501Serological ProfileHLA A: 2 11
HLA A ief: 2.2
HLA C: 6 7
HLA B: 57 #
Bw4/bw6: 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity East Asian gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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NRbM
Кат. номер 91073008-1VL 91073008-RNA-5UG 91073008-DNA-5UG Cell Line OriginMouse P3X63Ag8.653 x Mouse spleenCell Line DescriptionThe antibody reacts specifically with rabbit IgM.Culture MediumIDMEM + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon Mouse spleen Myeloma P3X63Ag8.653 Reactivity Anti-IgM, rabbit application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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NRK Cell Line from rat
Кат. номер 86032002-1VL 86032002-RNA-5UG 86032002-DNA-5UG Cell Line OriginRat kidney fibroblastCell Line DescriptionDerived from normal rat kidney (Rattus norvegicus). The cells are useful for focus-forming assays in virus titrations.ApplicationVirus titrationCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat kidney (fibroblast) growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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NRK-49F
Кат. номер 86101301-DNA-5UG 86101301-1VL 86101301-RNA-5UG Cell Line OriginRat kidney fibroblastCell Line DescriptionDerived from normal kidney and exhibits contact-inhibition. It is sensitive to viral or chemical transformation, including proteins such as SGF. Originated from the same mixed culture as NRK-52E but has distinct characteristics.ApplicationContact inhibition and transformation studiesCulture MediumDMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5-10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. The cells should be maintained sub-confluent, otherwise they will transform.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat kidney growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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NRK-52E
Кат. номер 87012902-1VL 87012902-RNA-5UG 87012902-DNA-5UG Cell Line OriginRat kidney epithelialCell Line DescriptionCloned from the same mixed culture of normal rat kidney cells as NRK-49F, but has epithelial-like morphology. It has distinct growth and transforming characteristics distinct from NRK-49F.ApplicationNRK-52E has been used to study the effects of altered selenium supply on the expression of endogenous thioredoxin reductase (TrxR).Culture MediumDMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5-10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Remove all traces of trypsin from cultures during sub-culturing i.e. spin cells or neutralise with serum.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat kidney growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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NS0 Cell Line from murine myeloma
Кат. номер 85110503-RNA-5UG 85110503-DNA-5UG 85110503-1VL Cell Line OriginMouse myelomaCell Line DescriptionNS0 is a non-Ig secreting, non-light chain-synthesising subclone of NS-1 (P3/NS1/1-Ag4-1 ECACC catalogue no. 85011427). The cells are resistant to 10μM azaguanine, cells die in the presence of HAT medium. The NS0 cell line is the property of the Medical Research Council (MRC). ECACC is unable to supply this cell line to commercial entities unless a license has been first secured from LifeArc (formerly known as MRC Technology). Requests for commercial use of this cell line will be forwarded to LifeArc.ApplicationNS0 Cell Line from murine myeloma has been used to culture NS0 cells expressing CD19scFv (a binding domain of B-lymphocyte antigen CD19) to screen hybridoma clones.
Fusion partnerCulture MediumRPMI 1640 (R0883), + 2mM L-Glutamine (G7513), + 10% FBS (F2442),Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells can adhere loosely to the culture vessel surface; remove by tapping against the flask. Cultures that are more than 2 days old may have to be scraped.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source mouse skin form cell suspension growth mode Suspension karyotype Not specified morphology Lymphoblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NS0-Serum-Free Cell Line from mouse
Кат. номер 3061601-DNA-5UG 3061601-1VL 3061601-RNA-5UG Cell Line OriginMouse myeloma, serum-freeCell Line DescriptionNS0 cell line (Sigma Catalogue no. 85110503) adapted to grow in the serum free medium EX-CELL® NS0 Serum-Free Medium for NS0 Cells (Sigma 14650C). Suitable for use as a fusion parent for serum-free generation of hybridomas. The NS0 cell line is the property of the Medical Research Council (MRC). ECACC is unable to supply this cell line or derivatives of this cell line to commercial entities unless a license has been first secured from LifeArc (formerly known as MRC Technology). Requests for commercial use of this cell line will be forwarded to LifeArc.ApplicationFusion partner for generation of serum-free hybridomas.Culture MediumEX-CELL®NS0 Serum-Free Medium for NS0 Cells (Sigma 14650C) + 8mM L-Glutamine (G7513),. When freezing cells down use EX-CELL®NS0 Serum-Free Medium for NS0 Cells (Sigma 14650C) + 8mM L-Glutamine (G7513), + 10% DMSO (D2650),.Subculture RoutineViability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 105 cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Legal InformationEX-CELL is a registered trademark of Sigma-Aldrich Co. LLCDisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source mouse blood description Mouse myeloma, serum-free growth mode Suspension karyotype Not specified morphology Lymphoblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NS0-Turbodoma
Кат. номер 6020202-DNA-5UG 6020202-1VL Cell Line OriginMouse myeloma, serum-free, without cholesterolCell Line DescriptionNS0 cell line (Sigma Catalogue no. 85110503) adapted to grow in the chemically defined protein- and peptide-free medium Turbodoma (Cell Culture Technologies). Cholesterol is not required as a supplement for this culture. This cell line is suitable for use as a fusion parent for serum-free generation of hybridomas. The NS0 cell line is the property of the Medical Research Council (MRC). ECACC is unable to supply this cell line or derivatives of this cell line to commercial entities unless a license has been first secured from LifeArc (formerly known as MRC Technology). Requests for commercial use of this cell line will be forwarded to LifeArc.Culture MediumTurbodoma medium (Cell Culture Technologies) + 2mM L-Glutamine (G7513),. When freezing cells down use TurboDoma™ medium (50:50 conditioned medium:fresh medium) + 2mM L-Glutamine (G7513), + 10% DMSO,.Subculture RoutineViability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. TranOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Legal InformationTurboDoma is a trademark of Cell Culture TechnologiesDisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source mouse blood growth mode Not specified karyotype Not specified morphology lymphoblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NS20Y
Кат. номер 8062517-RNA-5UG 8062517-DNA-5UG 8062517-1VL Cell Line OriginMouse neuroblastomaCell Line DescriptionThe cell line NS20Y is a cholinergic cell line derived from mouse neuroblastoma C1300 (Mouse strain A/Jax).Applicationcan be used as a neuronal modelCulture MediumDMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Not specified morphology Neuronal products Choline acetyltransferase receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NS20Y-BU-2
Кат. номер 8062502-RNA-5UG 8062502-1VL 8062502-DNA-5UG Cell Line OriginMouse neuroblastomaCell Line DescriptionA bromodeoxyuridine-resistant mutant of NS20Y (Sigma Catalogue number 08062517) The cell line NS20Y is a cholinergic cell line derived from mouse neuroblastoma C1300 Mouse strain A/Jax.ApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)+ 1 mM 8-bromodeoxyuridine. Cells can also be grown in the absence of bromodeoxyuridineSubculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Not specified morphology Neuronal products Choline acetyltransferase receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NS20Y-BU-4
Кат. номер 8062503-1VL 8062503-RNA-5UG 8062503-DNA-5UG Cell Line OriginMouse neuroblastomaCell Line DescriptionA bromodeoxyuridine-resistant mutant of NS20Y (Sigma Catalogue number 08062517) The cell line NS20Y is a cholinergic cell line derived from mouse neuroblastoma C1300 Mouse strain A/Jax.ApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)+ 1 mM 8-bromodeoxyuridine. Cells can also be grown in the absence of bromodeoxyuridineSubculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Not specified morphology Neuronal products Choline acetyltransferase receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
NS20Y-BU-5
Кат. номер 8062504-DNA-5UG 8062504-RNA-5UG 8062504-1VL Cell Line OriginMouse neuroblastomaCell Line DescriptionA bromodeoxyuridine-resistant mutant of NS20Y (Sigma Catalogue number 08062517) The cell line NS20Y is a cholinergic cell line derived from mouse neuroblastoma C1300 Mouse strain A/Jax.ApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)+ 1 mM 8-bromodeoxyuridine. Cells can also be grown in the absence of bromodeoxyuridineSubculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Not specified morphology Neuronal products Choline acetyltransferase receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену
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