RPMI 7932 cell line 94072246-1VL

Human pleural effusion from a patient with malignant melanoma
Derived from the pleural effusion of a 36 year old female with malignant melanoma. Cell line produces plasminogen activator. This cell line was found to be indistinguishable from cell lines COLO 699N & COLO 738 by STR PCR DNA profiling. At ECACC the STR PCR profile of all stocks of this cell line match the profile of the original source material received at ECACC. However, it is not clear if the cell lines COLO 699N, RPMI 7932 & COLO 738 were originally derived from the same individual as the profiles suggest. The cell lines COLO 699N, RPMI 7932 & COLO 738 were received from the same depositor. If we are able to obtain clarification of the relationship between these cell lines this information will be updated.
Tumourigenicity studies
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37°C.
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biological source	human lung (effusion)
Quality Level	100,
growth mode	Adherent
karyotype	Not specified
morphology	Epithelial-like
products	Plasminogen activator
receptors	Not specified
application(s)	cell culture | mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable