MEG-01

Кат. номер

94012401-1VL

94012401-RNA-5UG

94012401-CDNA-20UL

94012401-DNA-5UG

Human megakaryoblastic leukaemia
Derived from the bone marrow of a 55 year old male with chronic myelogenous leukaemia. All karyotypes show the Philadelphia chromosome. Platelet GP IIb/IIIa antigen is expressed on more than 92% of cells. Cytoplasmic expression of GP Ib antigen was not detected on the surface of cells but was present in the cytoplasm 20% of cells. BA-1 antigen is expressed in almost all cells. FVIII-related antigen was not detected on the surface cells but was present in cytoplasm.
Studies of physiological mechanisms governing the differentiation of human megakaryocyte and platelet release; the study of the biochemistry (and differentiation) of erythroid and myeloid cells.
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 8
D16S539: 9
D5S818: 13
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subcultures are prepared by diluting the cell suspension into fresh medium at a ratio of 1:2 to 1:3. Cells should be seeded at 2-4 x 105 cells/ml. Doubling time is 36-48 hours. 5% CO2; 37°C.
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biological source	human bone marrow
growth mode	Suspension
karyotype	Modal no. 56-58, hyperdiploid
morphology	Myoblast-like
products	Immunoglobulin G (IgG)-Fc in 12% of cells; C3′ in 53% of cells
receptors	Not specified
application(s)	cell culture | mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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