DOK

Кат. номер

94122104-DNA-5UG

94122104-1VL

94122104-RNA-5UG

94122104-CDNA-20UL

Human Caucasian dysplastic oral keratinocyte
The dysplastic oral keratinocyte cell line DOK was isolated from a piece of dorsal tongue from a 57 year old man. After a squamous-cell carcinoma was removed from the patient, who had been a heavy smoker, the remaining dysplasia was removed subsequently and used to initiate primary cultures leading to the establishment of DOK. The degree of dysplasia in the patient was described as mild to moderate. The cells show some stratification in confluent cultures and contain a keratin profile similar to the original dysplasia. Growth is stimulated by hydrocortisone and inhibited by cholera toxin. Expression of p53 is increased and a 12 bp in-frame deletion of the p53 gene (codon 188-191) could be identified. DOK cells are non-tumourigenic in athymic nude mice.
Study of oral keratinocytes, multi-stage oral carcinogenesis and carcinogens
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,13
D16S539: 9,12
D5S818: 12
D7S820: 11,13
THO1: 6,8
TPOX: 10
vWA: 14,18
DMEM + 2mM Glutamine + 5μg/ml Hydrocortisone + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

biological source	human mouth
growth mode	Adherent
karyotype	Aneuploid
morphology	Epithelial
products	p53
receptors	Not specified
application(s)	cell culture | mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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