DMS 153

Кат. номер

95062827-RNA-5UG

95062827-1VL

95062827-CDNA-20UL

95062827-DNA-5UG

Human Caucasian lung small cell carcinoma, metastasis to liver
DMS 153 was isolated from the liver of a 44 year-old male patient at autopsy who had been treated with cytoxan and methotrexate. The cells were identified as small cell carcinoma of the lung (SCLC) and secrete moderate quantities of calcitonin (CT) and calcitonin gene related peptide (CGRP). Production of adrenocorticotropin (ACTH), bombesin (BN), 17 β-estradiol and oxytocin-neurophysin (OT-NP) has also been reported. The expression of cell surface antigens Leu-7, HLA-DR, My 23 (defined by AML-2-23 monoclonal antibody), the AML-1-99 defined antigen and CR3 receptor (defined by the OKM antibody) has been shown. DMS 153 cells produce receptors for BN and epidermal growth factor (EGF) and are tumourigenic in athymic, nude mice.
Human lung tumour model
STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10
D13S317: 13
D16S539: 12,13
D5S818: 12
D7S820: 8,10
THO1: 7,9.3
TPOX: 11
vWA: 14,15
Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Cells grow in large clumps.
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biological source	human lung (liver metastasis)
growth mode	Semi-adherent aggregates
karyotype	Modal no. 41-42
morphology	Not specified
products	Not specified
receptors	Bombesin, Epidermal growth factor (EGF)
application(s)	cell culture | mammalian: suitable
relevant disease(s)	metastasis
shipped in	dry ice
storage temp.	−196°C

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