COLO 201

Кат. номер

87091201-DNA-5UG

87091201-RNA-5UG

87091201-1VL

87091201-CDNA-20UL

Human Caucasian colon adenocarcinoma
COLO 201, COLO 205 and COLO 206 (ECACC Catalogue 93052620) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). At time of isolation, COLO 201 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 201 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 10
D16S539: 12,13
D5S818: 10,13
D7S820: 9,10
THO1: 8,9
TPOX: 11
vWA: 15
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Maintain suspension cells at 3-9x105 cells/cm3. Split sub-confluent cultures (70-80%) of attached cells 1:3 to 1:6 i.e. seeding at 2-3x104 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
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biological source	human colon
growth mode	Adherent
karyotype	Triploid modal no. 71-78
morphology	Clumps of spherical cells in suspension, cuboidal cells in the monolayer
products	Carcinoembryonic antigen (CEA) less than produced by COLO 205
receptors	Not specified
application(s)	cell culture | mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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