C22 (Clara)

Кат. номер

7021401-1VL

7021401-DNA-5UG

Mouse lung epithelial progenitor cell
A progenitor cell of the peripheral airway epithelium derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only.
DMEM + 2 mM Glutamine + 4% Fetal Bovine Serum (FBS) + 8 supplements:
0.25 µg/ml Endothelin-1
0.01 µg/ml Interferon gamma
10 µg/ml Insulin
5 µg/ml Transferrin
7.5 µg/ml Endothelial Cell Growth Supplement (ECGS)
0.025 µg/ml Epidermal Growth Factor
0.36 µg/ml Hydrocortisone
0.02 µg/ml T3
For guidance on the supplements and preparation of the culture medium, ECACC can supply an information sheet on request - contact ECACC Technical Support.
Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using trypsin/EDTA;10% CO2; 33°C. Doubling time 48h
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	mouse lung
growth mode	Adherent
karyotype	Not specified
morphology	Epithelial
products	Not specified
receptors	Not specified
application(s)	cell culture | mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

RIDADR

NONH for all modes of transport