C10

Кат. номер

12022901-1VL

12022901-CDNA-20UL

12022901-DNA-5UG

12022901-RNA-5UG

Human colorectal adenocarcinoma, moderately well differentiated, Dukes′ stage B
The C10 cell line was established from a 71-year old male patient with moderately well differentiated adenocarcinoma of the descending colon classified as Dukes′ stage B.
STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 11
D13S317: 13
D16S539: 11
D5S818: 12
D7S820: 10,13
THO1: 6,9.3
TPOX: 8,9
vWA: 14,15
Iscove′s Modified Dulbecco′s Medium, + 10% Fetal Bovine Serum (FBS) + 2 mM Glutamine
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
C10 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100 g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

biological source	human colon
growth mode	Adherent
karyotype	Not specified
morphology	Epithelial
products	Not specified
receptors	Not specified
application(s)	cell culture | mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

RIDADR	NONH for all modes of transport
WGK Germany	3