BE(2)-C

Кат. номер

95011817-CDNA-20UL

95011817-RNA-5UG

95011817-1VL

95011817-DNA-5UG

Human Caucasian neuroblastoma
BE(2)-C is a clonal sub-line of SK-N-BE(2) (ECCAC catalogue no. 95011815) which was isolated from bone marrow of a 22-month-old male with disseminated neuroblastoma in 1972. They are reported to be multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine. The cells show a small, refractile morphology with short, neurite-like cell processes and tend to grow in aggregates.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Chemotherapy development, chromosome studies, drug resistance, differentiation studies.
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 6
TPOX: 11
vWA: 18
EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham′s F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).
Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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biological source	human nerve
growth mode	Adherent
karyotype	Modal no. 44
morphology	Neuroblast-like
products	Not specified
receptors	Not specified
application(s)	cell culture | mammalian: suitable
relevant disease(s)	metastasis
shipped in	dry ice
storage temp.	−196°C

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