108CC15

Кат. номер

8062516-1VL

8062516-DNA-5UG

8062516-RNA-5UG

Mouse neuroblastoma x Rat glioma hybrid
The cell line108CC15 is formed by the fusion of mouse neuroblastoma N18TG2 and rat glioma C6-BU-1using inactivated Sendai virus. This cell line was renamed NG108-15 by Klee and Nirenberg. Sigma has two catalogue entries for the 108CC15 cell line which differ in that they were deposited by different groups: 108CC15 from the original group that derived the cell line (Sigma Catalogue number 08062516) and NG108-15 (Sigma Catalogue number 88112302). Several other cell lines in the Sigma catalogue have been derived from 108CC15. This is a cholinergic hybrid cell line.
Used as a neuronal Model
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 days
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.
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biological source	Mouse x Rat Hybridoma nerve
growth mode	Adherent
karyotype	Modal No. varied with passage see reference
morphology	Neuronal
products	Long processes after treatment with dibutyryl cAMP, dense core vesicles, excitable membranes, neurotransmitter enzymes, synthesis of neurohormones and uptake of catecholamines.
receptors	Receptors for neurohormones: delta opioid, PGE1 vasoactive intestinal polypeptide, adenosine, somatostatin and acetylcholine.
application(s)	cell culture | mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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