1-7HB2

Кат. номер

10081201-RNA-5UG

10081201-CDNA-20UL

10081201-1VL

10081201-DNA-5UG

Breast cancer, morphology, adhesion properties
1-7HB2 is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7 identified by screening for branching morphology. The MTSV1-7 is a clonal non-tumourigenic cell line developed by immortalizing luminal epithelial cells cultured from milk using the SV40 T antigen. (See also MTSV1-7 CE1 Sigma catalog number 10081202.). Used for in vitro study of branching morphogenesis of human mammary epithelial cells and the role of integrins in mammary morphogenesis.
STR-PCR Data:
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 8,13
D5S818: 10,13
D7S820: 8,10
THO1: 7,9
TPOX: 11
vWA: 14,17
DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + Hydrocortisone (5 μg/ml) + Bovine Insulin (10 μg/ml).
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

biological source	human breast
growth mode	Adherent
karyotype	Not specified
morphology	Cuboidal
products	Formation of branching structures in the presence of fibroblast cultures or fibroblast-conditioned medium. Formation of spheroids in collagen type I gels.
receptors	Not specified
application(s)	cell culture | mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice

RIDADR	NONH for all modes of transport
WGK Germany	3