KAPA Taq ReadyMix with dye KK1024

NACRES:

NA.56

KAPA Taq DNA Polymerase is a single-subunit Taq DNA polymerase from the thermophilic bacterium Thermus aquaticus, purified from recombinant Escherichia coli. KAPA Taq ReadyMix™ (2X) is a ready-to-use cocktail containing all components for PCR, except primers and template. KAPA Taq ReadyMix is available with and without dye. The 2X ReadyMix with dye consists of two inert tracking dyes to permit direct loading of PCR products onto agarose gels for analysis by electrophoresis, without adding a DNA loading solution. KAPA Taq and KAPA Taq HotStart® DNA Polymerase have 53 polymerase and 53 exonuclease activities, but no 3 5 exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.
KAPA Taq ReadyMix™ with dye has been used in:

• high throughput PCR
• the amplification of low copy DNA templates
• multiplex PCR
• specific amplification of complex templates
• RT-PCR
• nested PCR (nPCR)
• amplifying exome 23 of anaplastic lymphoma kinase (ALK)
• polymerase chain reaction (PCR) amplification

Biochem/physiol Actions
KAPA Taq DNA Polymerase has 53 polymerase and 53 exonuclease activity. It does not elicit a 35 exonuclease (proofreading) activity. This enzyme system has an error rate of approximately 1 error per 2.2 X 105 nucleotides incorporated. PCR products created with KAPA Taq are A-tailed and are appropriate for cloning into TA cloning vectors.
Features and Benefits
Key Features:
High performance:

• Improved sensitivity, specificity, and yields
• Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product

Quick Notes:

• KAPA Taq ReadyMix™ can replace any commercial Taq DNA polymerase in an existing protocol. The annealing temperature may need to be optimized to account for differences in formulation.
• The KAPA Taq PCR system is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.
• The 2X KAPA Taq ReadyMix with dye includes two inert tracking dyes, which allow loading of PCR products directly onto agarose gels for analysis.
• KAPA Taq ReadyMixes contain 1.5 mM MgCl2 and 0.2 mM of each dNTP (at 1X).

Quality
Each batch of KAPA Taq DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq ReadyMixes are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Preparation Note
Handling:
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage.
For Research Use Only. Not for use in diagnostic procedures.
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

usage	50 μL sufficient for 250 reactions
Quality Level	100,
shelf life	≤18 mo.
feature	dNTPs included, hotstart: no
packaging	pkg of 6.25 mL
Торговая марка	Roche
concentration	2 x
application(s)	PCR: suitable
input	purified DNA
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash