KAPA LongRange

NACRES:

NA.55

Кат. номер

KK3501

KK3503

KK3502

The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long-range and/or sensitive PCR, and possess 3 – 4X higher fidelity than Taq polymerase. In the hot-start formulation, KAPA Long Range is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.
KAPA Long Range is also supplied in 2X ReadyMix format with loading dye that contains all the components required for PCR except primers and template. The loading dye in the reaction buffer allows you to load PCR products directly onto the agarose gel with no extra steps.
KAPA LongRange has been used in:

• Amplification of long targets and/or low amounts
• Standard short- and mid-range PCR
• T-A cloning
• Targeted sample enrichment for next-generation DNA sequencing
• polymerase chain reaction (PCR)

Biochem/physiol Actions
Both KAPA Taq HotStart® DNA polymerase and a modified archaeal (Type B) DNA polymerase enzyme possess 53 polymerase activity, but only KAPA Taq HotStart DNA polymerase possesses 53 exonuclease activity and only the Type B DNA polymerase possesses 35 exonuclease (proofreading) activity. The fidelity of KAPA LongRange HotStart is 2–4 fold higher than that of Taq DNA Polymerase, but is lower than the fidelity of pure proofreading polymerases such as KAPA HiFi. Reaction products generated with KAPA LongRange HotStart are polyA-tailed and can be cloned into TA-cloning vectors.
Features and Benefits
Amplify long targets with high sensitivity:

• Amplification of targets with high sensitivity and specificity up to 15 kb
• High yields with low input DNA

Outperforms with complex targets:

• Exhibits high yield, greater sensitivity and specificity where competitor long-range systems fail

Quick Notes:

• KAPA LongRange HotStart is a blend of KAPA Taq HotStart and a proofreading DNA polymerase.
• Suitable for short-, medium- and long-range PCR, and PCR with low amounts of template DNA.
• Fidelity is 2–4 times better than Taq.For short amplicons, replace Taq DNA polymerase for improvements in yield and/or fidelity.
• Amplify mid- to long-range targets with high yield and sensitivity.
• KAPA LongRange Buffer (Mg-free) is designed for long-range, sensitive PCR. MgCl2 is supplied separately for optimization.

Quality
Each batch of KAPA LongRange HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). The KAPA LongRange HotStart PCR system is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for longterm storage.
For Research Use Only. Not for use in diagnostic procedures.
HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Quality Level	100,
shelf life	≤18 mo.
feature	Long & Accurate PCR, dNTPs included, hotstart
packaging	pkg of 100 U (KK3501), pkg of 250 U (KK3502), pkg of 500 U (KK3503)
Торговая марка	Roche
application(s)	PCR: suitable
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash