Кат. номер |
1092020010 |
1.09202.0010 |
CT-Supplement contains cefixime and potassium tellurite. It is used in combination with Sorbitol MacConkey Agar (SMCA) to enhance the isolation of
Escherichia coli O157.
CT-Supplement is an additive for the preparation of CT-SMAC Agar for the isolation and differentiation of enterohemorrhagic (EHEC)
E. coli 0157:H7-strains from foodstuffs and stool.
Replaces: 1092020017
Analysis Note
Appearance
- clearness:
clear
- colour: colourless
pH-value (25 °C): 5.3 - 5.7
Typical composition (per vial): Cefixim 0.025 mg; Potassium Tellurite 1.25 mg.
Growth promotion test in accordance with ISO 11133.
Supplement tested with base Art. 1.09207.
Inoculum on reference medium
- Escherichia coli ATCC 35150 (O157):
10 - 100
- Escherichia coli O157:H7 ATCC 43894: 10 - 100
- Escherichia coli O157:H7 ATCC 43895: 10 - 100
Inoculum
- Escherichia coli ATCC 11775 (WDCM 00090):
>10
- Escherichia coli ATCC 25922 (WDCM 00013): >10
- Staphylococcus aureus ATCC 6538 (WDCM 00032): >10
- Staphylococcus aureus ATCC 25923 (WDCM 00034): >10
Recovery on test medium
- Escherichia coli ATCC 35150 (O157):
50%
- Escherichia coli O157:H7 ATCC 43894: 50%
- Escherichia coli O157:H7 ATCC 43895: 50%
Growth
- Escherichia coli ATCC 11775 (WDCM 00090):
partly inhibition
- Escherichia coli ATCC 25922 (WDCM 00013): partly inhibition
- Staphylococcus aureus ATCC 6538 (WDCM 00032): total inhibition
- Staphylococcus aureus ATCC 25923 (WDCM 00034): total inhibition
Colony colour
- Escherichia coli ATCC 35150 (O157):
colourless
- Escherichia coli O157:H7 ATCC 43894: colourless
- Escherichia coli O157:H7 ATCC 43895: colourless
- Escherichia coli ATCC 11775 (WDCM 00090): pink
- Escherichia coli ATCC 25922 (WDCM 00013): pink
Incubation: 24 hrs.; 37 °C; aerobic.
Reference: Tryptic-soy-agar. Inhibited strains tested with three-loop-streak.
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
storage conditions | Store at +2°C to +8°C. |
Quality Level | 200, |
composition | Cefixime, 0.0025 mg per vial ","Potassium tellurite, 1.25 mg per vial |
packaging | pkg of 10 vials |
application(s) | microbiological culture: suitable","microbiology: suitable |
Featured Industry | Food and Beverages |
compatibility | GranuCult® prime Sorbitol MacConkey (SMAC) Agar (1.00213) |
storage temp. | 2-8°C |
suitability | Escherichia coli |