Industrial Microbiology

Dissolve 10.5 g in 1 litre distilled water and adjust the pH to 7.4. Sterilize by autoclaving at 121°C for 15 minutes. Add 2 ml 1 M magnesium sulfate (Cat. No. 63133; filter sterilized) and 20 ml of 20% solution from a carbon source (such as glucose; Cat. No. 49140)

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
composition	ammonium chloride, 1 g/L , disodium hydrogen phosphate, 6 g/L , potassium dihydrogen phosphate, 3 g/L , sodium chloride, 0.5 g/L
Торговая марка	NutriSelect^® Basic
application(s)	microbiological culture: suitable, microbiology: suitable
final pH	7.4±0.2 (25 °C)
Featured Industry	Food and Beverages Life Science and Biopharma
suitability	nonselective for Escherichia coli, nonselective for coliforms

M9 Minimal Salts is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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M9, Minimal Salts, 5X

M9, Minimal Salts, 5X has been used:

in a selective agar medium for the isolation of sulphate reducing bacteria
in GMML minimal media to propagate phage-resistant DH10B cells
in M9 minimal medium which is used for cell motility assay

M9 Minimal Salts 5x are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for maintaining positive selection pressure on E. coli that produce essential amino acids and for maintenance of F-containing bacteria strains for M13 propagation.

1 kg in poly bottle

Features and Benefits

M9 powder provides:

Standard formulation
A budget-friendly alternative to liquid

1. Dissolve 56.4 g in 1L of distilled water.
2. Autoclave for 15 minutes at 121°C.
This convenient 5x concentrate can be stored and diluted as needed to prepare 5 L of 1x M9 minimal salts.
For M9 minimal medium:
1. Aseptically dilute 200 ml of M9 minimal salts, 5x concentrate with 800 ml of sterile water. If necessary, cool to 45-50°C.
2. Aseptically add 20 ml of sterile 1 M glucose and 2 ml of sterile 1 M magnesium sulfate to prepare 1 L of M9 minimal medium.
3. If desired, aseptically add 0.1 ml of 1 M sterile calcium chloride to the M9 minimal medium. The M9 minimal medium may also be supplemented with the appropriate amino acids.

Reconstitution

Make a 5x concentrated stock solution by stirring to suspend 56.4g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Dilute the 5x M9 concentrated stock to a 1x working solution by adding 200mL 5x M9 stock to 800mL sterile water. If needed, allow to cool before making additions, such as antibiotics (if desired).

Quality Level	200
grade	for molecular biology
sterility	non-sterile
form	powder
composition	KH₂PO₄, 15 g/L , NaCl, 2.5 g/L , Na₂HPO₄, 33.9 g/L , NH₄Cl, 5 g/L
Featured Industry	Food and Beverages
storage temp.	room temp
suitability	nonselective for Escherichia coli, nonselective for coliforms

Mac Conkey-Sorbitol ChromoSelect Agar is recommended for selective isolation of Escherichia coli 0157:H7 from food and animal feeding stuffs. MacConkey Sorbitol Agar is based on the formulation described by Rappaport and Henigh. The medium contains sorbitol instead of lactose and it is recommended for the detection of enteropathogenic strains of E. coli 0157:H7 which ferments lactose but does not ferment sorbitol and hence produce colorless colonies. Sorbitol fermenting strains of E. coli produce pink-red colonies. The red color is due to production of acid from sorbitol, absorption of neutral red and a subsequent color change of the dye when pH of the medium falls below 6.8. E. coli 0157:H7 has been recognised as a cause of haemorrhagic colitis. B.C. indicator is added to detect the presence of an enzyme -D-glucuronidase which is specific for E. coli. Stranins of E. coli possessing -D-glucuronidase appear as blue colored colonies on the medium. Enteropathogenic strains of E. coli 0157 do not possess beta-D-glucuronidase activity and thus produce colorless colonies. E. coli fermenting sorbitol and possessing beta-D-glucuronidase activity produce purple colored colonies. Most of the gram-positive organisms are inhibited by crystal violet and bile salts. Addition of Tellurite-Cefixime Supplement makes the medium selective. Potassium Tellurite selects the serogroups 0157 from other E. coli serogroups and inhibits Aeromonas species and Providencia species. Cefixime inhibits Proteus species.

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US)
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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Mac Conkey-Sorbitol ChromoSelect Agar

Suspend 50.1g in 1 litre distilled water. Boil gently to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50°C. Mix well and pour into sterile petri plates. If desired add Tellurite-Cefixime Supplement to the molten and cooled medium (50°C) before pouring into sterile petri plates.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 13.5 g/L , B.C. indicator, 0.1 g/L , bile salts mixture, 1.5 g/L , casein enzymic hydrolysate, 17 g/L , crystal violet, 0.001 g/L , neutral red, 0.03 g/L , proteose peptone, 3 g/L , sodium chloride, 5 g/L , sorbitol, 10 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiology: suitable (microbial identification)
final pH	7.1±0.3 (25 °C)
Featured Industry	Agriculture Environmental Food and Beverages
suitability	selective and differential for Escherichia coli, selective and differential for coliforms, selective and differential for enterobacteriaceae, selective and differential for enterovirulent E. coli (EEC)

MacConkey Agar has been used to differentiate members of the coliform group It has also been used to plate serial dilutions of fecal and colonic homogenates of Citrobacter rodentium.

hcodes	H412
pcodes	P273 - P501
Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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MacConkey Agar

EC Number:	232-658-1
MDL number:	MFCD00147502
eCl@ss:	42040102
NACRES:	NA.74

250, 500 g in poly bottle

Biochem/physiol Actions

MacConkey Agar is considered as a selective and differential media. It is useful in differentiating between the lactose fermenting and non-lactose fermenting bacteria. Thus, it is useful in the isolation of gram negative bacteria. Primary plating medium is used to differentiate members of the coliform group.

Suspend 50 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Pour into sterile petri plates. Dry the surface of the gel before inoculation.

Microbiologically tested.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
Торговая марка	NutriSelect^® Basic
application(s)	microbiology: suitable (microbial identification), water monitoring: suitable
Featured Industry	Agriculture Environmental Food and Beverages Pharmaceutical
suitability	Citrobacter spp., Escherichia coli, Gardnerella spp., coliforms

A differential plating medium recommended for the detection and isolation of coliforms and intestinal pathogens from stool, urine, water and other material.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1

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MacConkey Agar No 1

Suspend 52 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Pour into sterile petri plates. Dry the surface of the gel before inoculation.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200,
sterility	non-sterile
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 12.0 g/L , bile salts, 5.0 g/L , lactose, 10.0 g/L , neutral red, 0.075 g/L , peptone, 20.0 g/L , sodium chloride, 5.0 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable (microbial identification), water monitoring: suitable
final pH	7.4±0.2 (25 °C)
Featured Industry	Agriculture Clinical Testing Environmental Food and Beverages
suitability	selective and differential for Campylobacter spp., selective and differential for Enterobacter spp., selective and differential for Enterococcus spp., selective and differential for Escherichia coli, selective and differential for Proteus spp., selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for Staphylococcus spp., selective and differential for coliforms, selective and differential for enterobacteriaceae

A differential media recommended for the selection and recovery of Enterobacteriaceae.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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MacConkey Agar with Crystal violet, Sodium chloride and 0.15% Bile salts

Components (g/L)
Peptic digest of animal tissue 20.0
Lactose 10.00
Bile salts 1.50
Sodium chloride 5.00
Crystal violet 0.001
Neutral red 0.05
Agar 15.00

Suspend 51.55 g of MacConkey Agar with Crystal Violet, Sodium Chloride and 0.15% Bile Salts in 1000 ml of distilled water. Heat to boiling, with gentle swirling to dissolve the agar completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45-50°C and pour into sterile petri plates.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable (microbial identification)
pH	7.2±0.2 (25 °C)
Featured Industry	Environmental Food and Beverages
suitability	selective and differential for Enterobacter spp., selective and differential for Enterococcus spp., selective and differential for Escherichia coli, selective and differential for Klebsiella spp., selective and differential for Proteus spp., selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for Streptococcus spp., selective and differential for coliforms, selective and differential for enterobacteriaceae

A presumptive medium for the detection of the coliform group in water and milk.

hcodes	H412
pcodes	P273 - P501
Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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MacConkey Broth

Add 40 g to 1 litre of distilled water. Mix well and distribute into containers fitted with Durham tubes. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Recommendations of the Public Health Laboratory Service Water Subcommittee

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	bile salts, 5.0 g/L , lactose, 10.0 g/L , neutral red, 0.075 g/L , peptone, 20.0 g/L , sodium chloride, 5.0 g/L
Торговая марка	NutriSelect^® Plus
application(s)	bioburden testing: suitable, microbiology: suitable (microbial identification), water monitoring: suitable
final pH	7.2±0.2 (25 °C)
Featured Industry	Agriculture Clinical Testing Environmental Food and Beverages
suitability	selective and differential for Enterobacter spp., selective and differential for Escherichia coli, selective and differential for Klebsiella spp., selective and differential for Proteus spp., selective and differential for Salmonella spp., selective and differential for coliforms, selective and differential for enterobacteriaceae

MacConkey Broth Purple is a differential medium used for the detection of coliforms in water samples and milk examination by MPN technique. It is a modification of the classic medium, where neutral red is replaced by a less aggressive indicator, according European Pharmacopoeia. It is also recommended by ISO Committee under the specification ISO 9308-2:1990.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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MacConkey Broth Purple

Suspend 40 g in 1000 ml distilled water. Dispense in test tubes with inverted Durham tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200,
sterility	non-sterile
form	powder
shelf life	limited shelf life, expiry date on the label
composition	bile salts, 5.0 g/L , bromoresol purple, 0.01 g/L , lactose, 10.0 g/L , peptone, 20.0 g/L , sodium chloride, 5.0 g/L
Торговая марка	NutriSelect^® Plus
application(s)	bioburden testing: suitable, microbiology: suitable (microbial identification), water monitoring: suitable
final pH	7.4±0.2 (25 °C)
Featured Industry	Agriculture Environmental Food and Beverages
suitability	selective and differential for Citrobacter spp., selective and differential for Enterobacter spp., selective and differential for Escherichia coli, selective and differential for Klebsiella spp., selective and differential for Proteus spp., selective and differential for Salmonella spp., selective and differential for Serratia spp., selective and differential for Shigella spp., selective and differential for coliforms, selective and differential for enterobacteriaceae

For the isolation of Salmonella, Shigella and coliform bacteria, in particular E. coli, from diverse material.
Bile salts and crystal violet extensively inhibit the Gram-positive flora. The presence of lactose and neutral red indicate lactose-positive colonies from which E. coli can be identified by fluorescence in the UV.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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MacConkey MUG Agar

Dissolve 50.1 g in 1 litre distilled water. Autoclave at 121°C for 15 minutes.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 13.5 g/L , bile salt mixture, 1.5 g/L , casein peptone, 17 g/L , crystal violet, 0.001 g/L , lactose, 10 g/L , meat peptone, 3 g/L , 4-methylumbelliferyl--D-glucuronide, 0.1 g/L , neutral red, 0.03 g/L , sodium chloride, 5 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable (microbial identification), water monitoring: suitable
final pH	7.1±0.2 (25 °C)
Featured Industry	Agriculture Clinical Testing Environmental Food and Beverages
suitability	selective and differential for Enterobacter spp., selective and differential for Escherichia coli, selective and differential for Proteus spp., selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for coliforms, selective and differential for enterobacteriaceae

A differential medium which restricts the swarming of most Proteus species. Useful for culturing urine specimens which may contain large number of Proteus species.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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MacConkey-Agar (without salt)

Suspend 47 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Pour into sterile petri plates. Dry the surface of the gel before inoculation.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 12 g/L , bile salts, 5 g/L , lactose, 10 g/L , neutral red, 0.075 g/L , peptone, 20 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable (microbial identification)
pH	7.4±0.2 (25 °C)
Featured Industry	Clinical Testing Environmental Food and Beverages
suitability	Acinetobacter spp., selective and differential for Enterococcus spp., selective and differential for Escherichia coli, selective and differential for Proteus spp., selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for Staphylococcus spp., selective and differential for coliforms, selective and differential for enterobacteriaceae

MacConkey-Sorbitol Agar is a growth media, which is used for the selective detection and differentiation of Escherichia coli in water samples.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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MacConkey-Sorbitol Agar

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Agency/Method	according to ISO 16654:2001
Quality Level	100,
sterility	non-sterile
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 13.5 g/L , bile salts, 1.5 g/L , crystal violet, 0.001 g/L , neutral red, 0.03 g/L , peptone, 17 g/L , proteose peptone, 3 g/L , sodium chloride, 5 g/L , sorbitol, 10 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiology: suitable (microbial identification)
final pH	7.1±0.2 (25 °C)
Featured Industry	Agriculture Environmental Food and Beverages
suitability	selective and differential for Escherichia coli, selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for coliforms, selective and differential for enterobacteriaceae, selective and differential for enterovirulent E. coli (EEC)

Malachite Green Broth is for the selective enrichment of Pseudomonas aeruginosa according to Habs and Kirschner.
The culture medium has been recommended by Schubert and Blum for the examination of ground-, surface-, drinking-, bathing and process-water. In addition, it is suitable for the examination of mineral and spring-water.

hcodes	H412
pcodes	P273 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Malachite Green Broth

PubChem Substance ID:

Malachite Green Broth has been used for the growth of Pseudomonas aeruginosa from water samples.
For the selective enrichment of Pseudomonas aeruginosa according to Habs and Kirschner.

Dissolve 25 g in 1 litre distilled water. Pour into small containers and autoclave at 121°C for 15 minutes.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	dipotassium hydrogen phosphate, 1.1 g/L , malachite green oxalate, 0.03 g/L , meat extract, 9 g/L , meat peptone, 15 g/L
Торговая марка	NutriSelect^® Basic
application(s)	bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable, sample preparation: suitable, water monitoring: suitable
final pH	7.0±0.2 (25 °C)
Featured Industry	Environmental Food and Beverages
storage temp.	2-8°C
suitability	selective for Pseudomonas spp.

Used for the detection and isolation of yeasts and molds from dairy products and foods.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Malt agar

Suspend 45 g in 1000 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Avoid overheating as it will result in a softer and darker agar.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	Agar, 15.00 g/L , Malt extract, 30.00 g/L
Торговая марка	NutriSelect^® Plus
application(s)	bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable
pH	5.5±0.2 (25 °C)
Featured Industry	Clinical Testing Food and Beverages
suitability	selective by low pH for Aspergillus spp., selective by low pH for Candida spp., selective by low pH for Penicillium spp., selective by low pH for Pichia spp., selective by low pH for Saccharomyces spp., selective by low pH for Zygosaccharomyces spp.

A medium for the isolation and enumeration of yeasts and molds.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Malt Extract Agar

eCl@ss:	42040102
NACRES:	NA.74

Suspend 50 g in 1 litre of distilled water and bring to the boil to dissolve. Sterilize by autoclaving at 115°C for 10 minutes. For inhibition of bacterial growth, 2 ml of a 10% sterile filtered lactic acid can be added to adjust the pH to 3.5.

Other Notes

Biological attributes of colony-type variants of Candida albicans; Volatile metabolites and other indicators of P. aurantiogriseum - growth on different substrates

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15 g/L , malt extract, 30 g/L , mycological peptone, 5 g/L
Торговая марка	NutriSelect^® Plus
application(s)	bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable
final pH	5.4±0.2 (25 °C)
Featured Industry	Agriculture Clinical Testing Environmental Food and Beverages
suitability	selective by low pH for Aspergillus spp., selective by low pH for Candida spp., selective by low pH for Penicillium spp., selective by low pH for Pichia spp., selective by low pH for Saccharomyces spp., selective by low pH for Zygosaccharomyces spp., selective by low pH for molds (General Media), selective by low pH for yeasts (General Media)

This medium is recommended for isolation, detection and enumeration of yeasts and molds.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Malt Extract Agar modified, Vegitone

Suspend 31.28 g in 1000 ml distilled water. Add 2.35 g glycerol (Cat. No. 49767). Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15.00 g/L , dextrin, 2.75 g/L , maltose, 12.75 g/L , peptone (vegetable), 0.78 g/L
Торговая марка	NutriSelect^® Plus
application(s)	bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable
final pH	4.7±0.2 (25 °C)
Featured Industry	Agriculture Clinical Testing Environmental Food and Beverages Pharmaceutical
suitability	selective by low pH for Aspergillus spp., selective by low pH for Candida spp., selective by low pH for Penicillium spp., selective by low pH for Pichia spp., selective by low pH for Saccharomyces spp., selective by low pH for Zygosaccharomyces spp., selective by low pH for molds (General Media), selective by low pH for yeasts (General Media)

for isolation, detection and enumeration of yeasts and molds

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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Malt Extract Agar, modified

Suspend 31.28 g of Malt Extract Agar, Modified in 1000 ml of distilled water and add 2.35 g of glycerol (Cat. No. 49767). Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. AVOID OVERHEATING! The low pH (4.7) of the medium may catalyze the hydrolysis of the agar during prolonged heating, resulting in a softer gel or failure of the agar to gel.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

form	powder
Quality Level	100,
composition	agar, 15 g/L , dextrin, 2.75 g/L , maltose, 12.75 g/L , peptic digest of animal tissue, 0.78 g/L
Торговая марка	NutriSelect^® Plus
application(s)	bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable
Featured Industry	Agriculture Clinical Testing Environmental Food and Beverages Pharmaceutical
suitability	selective by low pH for Aspergillus spp., selective by low pH for Candida spp., selective by low pH for Penicillium spp., selective by low pH for Pichia spp., selective by low pH for Saccharomyces spp., selective by low pH for Zygosaccharomyces spp., selective by low pH for molds (General Media), selective by low pH for yeasts (General Media)

A liquid medium for the cultivation of yeasts and molds.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Malt Extract Broth

Add 20 g to 1 litre of distilled water. Mix well, distribute into final containers and sterilize by autoclaving at 115°C for 10 minutes.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	malt extract, 17 g/L , mycological peptone, 3 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable, sample preparation: suitable
final pH	5.4±0.2 (25 °C)
Featured Industry	Agriculture Clinical Testing Environmental Food and Beverages Life Science and Biopharma
suitability	nonselective for Aspergillus spp., nonselective for Candida spp., nonselective for Penicillium spp., nonselective for Pichia spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp., nonselective for molds (General Media), nonselective for yeasts (General Media)

Mannitol Lysine Agar is used for selective isolation of Salmonella species other than Salmonella Typhi and Salmonella Paratyphi A.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Mannitol Lysine Crystal Violet Brilliant Green Agar

Suspend 49.02 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15.0 g/L , beef extract, 2.0 g , briliant green, 0.0125 g/L , crystal violet, 0.010 g/L , ferric ammonium citrate, 1.0 g/L , L-lysine hydrochloride, 5.0 g/L , mannitol, 3.0 g/L , peptic digest of animal tissue, 10.0 g/L , sodium chloride, 4.0 g/L , sodium thiosulfate, 4.0 g/L , yeast extract, 5.0 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable
final pH	6.8±0.2 (25 °C)
Featured Industry	Clinical Testing Food and Beverages Veterinary
suitability	nonselective and differential for Salmonella spp.

Selective agar for the detection of pathogenic staphylococci in food and other material acc. to Chapman (modified).

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Mannitol Salt Phenol Red Agar

Dissolve 111 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Pour plates.

Other Notes

Primary screening medium for detection and identification of methicillin-resistant Staphylococcus aureus; Modified medium for the recovery of Staphylococcus from water

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15.0 g/L , D-mannitol, 10.0 g/L , meat extract, 1.0 g/L , peptone, 10.0 g/L , phenol red, 0.025 g/L , sodium chloride, 75.0 g/L
Торговая марка	NutriSelect^® Basic
application(s)	bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable
final pH	7.4±0.2 (25 °C)
Featured Industry	Clinical Testing Environmental Food and Beverages Veterinary
suitability	nonselective and differential for Staphylococcus spp.

Marine Broth 2216 is used for cultivating heterotrophic marine bacteria.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Marine Broth 2216

Suspend the powder in 1 L of purified water: Marine Broth 2216 - 37.4 g. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Autoclave at 121°C for 15 minutes.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	ammonium nitrate, 1.6 mg/L , boric acid, 22.0 mg/L , calcium chloride, 1.8 g/L , disodium phosphate, 8.0 mg/L , ferric citrate, 0.1 g/L , magnesium chloride, 5.9 g/L , magnesium sulfate, 3.24 g/L , peptone, 5.0 g/L , potassium bromide, 0.08 g/L , potassium chloride, 0.55 g/L , sodium bicarbonate, 0.16 g/L , sodium chloride, 19.45 g/L , sodium fluoride, 2.4 mg/L , sodium silicate, 4.0 mg/L , strontium chloride, 34.0 mg/L , yeast extract, 1.0 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiology: suitable
final pH	7.6±0.2 (25 °C)
Featured Industry	Food and Beverages
suitability	Trichophyton spp., marine bacteria

Maximum Recovery Diluent is a protective and isotonic medium used for maximal recovery of microorganisms from a variety of sources. The low concentration of peptone does not cause multiplication of the organisms within 1-2 hours of dilution of the sample.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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Maximum Recovery Diluent

Suspend 9.5 g in 1000 ml of purified water. Heat if necessary to dissolve the medium completely. Sterilize at 121°C for 15 minutes.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Agency/Method	according to ISO 6887-1:2017, according to ISO 8199:2018
Quality Level	200,
sterility	non-sterile
form	powder
shelf life	limited shelf life, expiry date on the label
composition	peptic digest of animal tissue, 1.0 g/L , sodium chloride, 8.5 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiology: suitable, sample preparation: suitable
final pH	7.0±0.2 (25 °C)
Featured Industry	Clinical Testing Environmental Food and Beverages Pharmaceutical
suitability	nonselective for bacteria (General Media)

GranuCult is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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mCCD (Modified charcoal cefoperazone deoxycholate) agar (base)

Appearance (colour): black
pH-value (25°C): 7.2 - 7.6
Typical composition (g/litre): Peptones 20.0; Casein hydrolysate 30.; Activated charcoal 4.0; Sodium deoxycholate 1.0; Sodium pyruvate 0.25; Ferrous sulfate 0.25; Agar-agar 12.0.
Growth promotion test in accordance with ISO 11133.
Base tested with supplement Art. 1.00071.
Growth (Campylobacter coli ATCC 43478 (WDCM 00004)): good
Growth (Campylobacter jejuni ATCC 33291 (WDCM 00005)): good
Growth (Campylobacter jejuni ATCC 29428 (WDCM 00156)): good
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): no growth
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): inhibited growth
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): inhibited growth
Incubation: 44 hrs. 41.5 °C with Anaerocult C. E.coliand Staph.aur. aerobic.
Strains tested with three-loop-streak.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

For the cultivation of anaerobic microorganisms.

storage conditions	Store at +15°C to +25°C.
Quality Level	200,
Agency/Method	ISO
form	powder (dehydrated (DCM))
Торговая марка	GranuCult^®
application(s)	microbiological culture: suitable, microbiology: suitable, pathogen testing: suitable
pH	7.2-7.6 (25 °C, 44.5 g/L in H₂O)
solubility	45.5 g/L
Featured Industry	Food and Beverages
storage temp.	15-25°C
suitability	Campylobacter spp.

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Meat Liver Agar

Dissolve 34.2 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 11.0 g/L , ammonium ferric citrate, 0.5 g/L , D(+)-glucose, 0.75 g/L , meat liver base, 20.0 g/L , sodium sulfite, 1.2 g/L , starch, 0.75 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable
final pH	7.6±0.2 (25 °C)
Featured Industry	Clinical Testing Environmental Food and Beverages
suitability	anaerobic bacteria, nonselective and differential for Clostridium spp.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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mEC-broth with Novobiocin

Кат. номер
1145820500
1145825000
US1114582/5000
US1114582/0500
1145820507
1.14582.0500
1145825007

Appearance (clearness): clear
Appearance (color): yellowish to yellowish-braun
pH-value (25°C): 6.7 - 7.1
Inoculum on reference medium (Escherichia coli ATCC 35150 (O157)): 100
Inoculum on reference medium (Escherichia coli ATCC 11775 (WDCM 00090)): 100
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): 10000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): 10000
Inoculum on reference medium (Proteus hauseri ATCC 13315): 10000
Growth (Escherichia coli ATCC 35150 (O157)): >10 transparent colonies with a pale yellowish-brown appearance on SMAC agar
Growth (Escherichia coli ATCC 11775 (WDCM 00090)): >10 pink colonies on SMAC Agar
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition on TSA
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition on TSA
Mixed inoculum of E.coli, P.hauseri and E.faecalis
Additionaly monoculture of E.faecalis
Confirmation on SMAC agar resp. Tryptic soy agar
Incubation:
mEC broth: 18 - 24 hours at 41,5 ± 1 °C aerobic
Confirmation on SMAC agar resp. Tryptic Soy Agar: 18 - 24 hours at 35 ± 1 °C aerobic

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Store at +15°C to +25°C.
Quality Level	200,
form	medium granules (dehydrated (DCM))
Торговая марка	GranuCult^®
application(s)	microbiological culture: suitable, microbiology: suitable, pathogen testing: suitable
pH	6.7-7.1 (25 °C, 36.7 g/L in H₂O, after autoclaving)
solubility	36.7 g/L
Featured Industry	Food and Beverages
storage temp.	15-25°C
suitability	Escherichia coli

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mEHEC Broth in bags 120569-2X5L

Culture medium for selectivity enriching Salmonella, Enterohemorrhagic E.coli O157:H7, and non-O157 Shiga toxin-producing E. coli in selected foods. Specially validated for use with Assurance® GDS, TRANSIA™ Plate, TRANSIA™ AG, and VIP® Gold.

mEHEC Media contains following nutrients sources: casein digest, yeast digest, carbohydrates, and salts. The exact composition of this proprietary media cannot be disclosed.

Assurance is a registered trademark of BioControl Systems, Inc.
TRANSIA is a trademark of BioControl Systems, Inc.
VIP is a registered trademark of BioControl Systems, Inc.

form	liquid
shelf life	limited shelf life, expiry date on the label
feature	ready-to-use
application(s)	microbiology: suitable, pathogen testing: suitable
Featured Industry	Food and Beverages Industrial QC
suitability	enterovirulent E. coli (EEC)

Culture medium for selectivity enriching Enterohemorrhagic E.coli O157:H7, non-O157 Shiga toxin-producing E. coli, and Salmonella in selected foods. For use with Assurance® GDS, TRANSIA™ Plate, TRANSIA™ AG, and VIP® Gold.

WGK Germany

WGK 3

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mEHEC®

1 bucket containing 10 Kg of dehydrated mEHEC media (65012-10K) or 1 x 500 g container of dehydrated mEHEC media (65012-500)

Assurance is a registered trademark of BioControl Systems, Inc.
TRANSIA is a trademark of BioControl Systems, Inc.
VIP is a registered trademark of BioControl Systems, Inc.
mEHEC is a registered trademark of BioControl Systems, Inc.

sterility	non-sterile
form	powder
shelf life	636 mo.
Торговая марка	BioControl Systems 65012-10K, BioControl Systems
storage condition	dry media
application(s)	microbiological culture: suitable, pathogen testing: suitable, sample preparation: suitable
Featured Industry	Food and Beverages
compatibility	for use with Assurance GDS^® Tq from BioControl Systems, Inc., for use with TRANSIA^® AG (4000BC), for use with TRANSIA^® AG (61001BC), for use with TRANSIA^® AG (67000BC), for use with VlP^® Gold (60036BC), for use with VlP^® Gold (60037BC), for use with VlP^® Gold (60038BC)
storage temp.	room temp
suitability	Salmonella spp., enterovirulent E. coli (EEC)

A chromogenic medium for the differentiation and enumeration of Escherichia coli and other coliforms, which simplifies the membrane filtration technique by reducing the number of filtration stages and the need for further confirmation steps. This medium is described in the Environment Agencys report of UK, Methods for Examination of Waters and Associated Material - The Microbiology of Drinking Water (2002) - Part 4 - Methods for the isolation and enumeration of coliform bacteria and Escherichia coli (including E. coli O157:H7).

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Membrane Lactose Glucuronide Agar

Suspend 88g in 1 litre of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and pour into sterile Petri dishes.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 10 g/L , X-glucuronide (BCIG), 0.2 g/L , lactose, 30 g/L , peptone, 40 g/L , phenol red, 0.2 g/L , sodium lauryl sulfate, 1.0 g/L , sodium pyruvate, 0.5 g/L , yeast extract, 6 g/L
Торговая марка	NutriSelect^® Plus
application(s)	bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable, water monitoring: suitable
final pH	7.4±0.2 (25 °C)
Featured Industry	Agriculture Clinical Testing Environmental Food and Beverages
suitability	selective and differential for Enterobacter spp., selective and differential for Escherichia coli, selective and differential for Klebsiella spp., selective and differential for coliforms, selective and differential for enterovirulent E. coli (EEC)

Appearance (clearness): clear
Appearance (color): yellow
pH-value (25°C): 6.7 - 7.1
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): +
Growth (Streptococcus pyogenes ATCC 12344): +
Growth (Enterococcus faecalis ATCC 11700): +
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): +
Growth (Citrobacter freundii ATCC 8090): +
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): +
Incubation: 18 - 24 hrs.; 35 °C; aerobic streaked on Standard I nutrient agar

Personal Protective Equipment	Eyeshields,, Faceshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Membrane-filter rinse fluid (USP)

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

MeReSa ChromoSelect Agar Base is recommended for the isolation and selective identification of Methicillin Resistant Staphylococcus aureus (MRSA) from clinical isolates.

storage conditions	Store at +15°C to +25°C.
form	medium granules (dehydrated (DCM))
Quality Level	200,
Торговая марка	GranuCult^®
application(s)	microbiological culture: suitable, microbiology: suitable
Featured Industry	Pharmaceutical
storage temp.	15-25°C

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MeReSa ChromoSelect Agar Base

Suspend 41.65 g in 500 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C and aseptically add the rehydrated contents of 1 vial of MRSA Selective Supplement (Cat. No. 51387). Mix well and pour into sterile petri plates.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15.0 g/L , beef extract, 2.5 g/L , casein enzymic hydrolysate, 13.0 g/L , chromogenic mixture, 5.3 g/L , sodium chloride, 40.0 g/L , sodium pyruvate, 5.0 g/L , yeast extract, 2.5 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiology: suitable (microbial identification)
final pH	7.0±0.2 (25 °C)
Featured Industry	Clinical Testing Food and Beverages
storage temp.	2-8°C
suitability	selective and differential for Staphylococcus spp.

A classic liquid medium recommended for the Methyl-red and Voges-Proskauer tests for the differentiation of the coli-aerogenes group according ISO standards 6579 and 6585 and FIL - IDF 93 standard.

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
Personal Protective Equipment	Eyeshields,, Gloves,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Methyl Red Voges Proskauer Broth

Dissolve 17 g of in 1 litre of distilled water. Mix well, distribute into test tubes in amounts of 10 ml and sterilise by autoclaving at 121°C for 15 minutes.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200,
Agency/Method	according to GB 4789.30-2016, according to ISO 11290-1:2017, according to ISO 11290-2:2017, according to ISO 22964:2017
sterility	non-sterile
product line	BioChemika
form	powder
shelf life	limited shelf life, expiry date on the label
composition	bufferes peptone, 7 g/L , dextrose, 5 g/L , dipotassium phosphate, 5 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbe id \| metabolite detection: suitable, microbiological culture: suitable, microbiology: suitable (microbial identification)
final pH	6.9±0.2 (25 °C)
Featured Industry	Agriculture Clinical Testing Environmental Food and Beverages
suitability	nonselective for Citrobacter spp., nonselective for Escherichia coli, nonselective for Klebsiella spp., nonselective for Proteus spp., nonselective for Salmonella spp., nonselective for Yersinia spp., nonselective for coliforms, nonselective for enterobacteriaceae

MGYP Agar with Copper is a selective medium recommended for isolation and cultivation of wild yeasts in the brewing industry.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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MGYP Agar with Copper

Suspend 41.4 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 20.0 g/L , cupric sulfate, 0.4 g/L , dextrose, 10.0 g/L , gelatin peptone, 5.0 g/L , malt extract, 3.0 g/L , yeast extract, 3.0 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiology: suitable (microbial identification)
final pH	6.2±0.2 (25 °C)
Featured Industry	Food and Beverages
suitability	Candida spp., Pichia spp., Saccharomyces spp., Zygosaccharomyces spp.

Middlebrook 7H10 Agar Base has been used in the culturing of Mycobacterium immunogenum. It has also been used in the drug susceptibility testing of Mycobacterium tuberculosis. It is also recommended for isolation and cultivation of Mycobacterium tuberculosis.

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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Middlebrook 7H10 Agar Base

Ingredients (g/L)
Ammonium sulfate, 0.50
L-Glutamic acid, 0.50
Monopotassium phosphate, 1.50
Disodium phosphate, 1.50
Sodium citrate, 0.40
Ferric ammonium citrate, 0.04
Magnesium sulfate, 0.025
Calcium chloride, 0.0005
Zinc sulfate, 0.001
Copper sulfate, 0.001
Pyridoxine hydrochloride, 0.001
Biotin, 0.0005
Malachite green, 0.00025
Agar, 15.00

Suspend 19.47 g of Middlebrook 7H10 Agar Base in 900 ml of distilled water. Add 5 ml of glycerol (Cat. No. 49767). Boil to dissolve the medium completely. Distribute in 180 ml amounts in erlenmeyer flasks and sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes. Cool to 45°C and aseptically add 20 ml of Middlebrook OADC Growth Supplement (Cat. No. M0678). Mix well and pour into screw capped tubes.Note: Keep prepared medium in the dark before and after inoculation.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
product line	BioChemika
form	powder
shelf life	limited shelf life, expiry date on the label
Торговая марка	NutriSelect^® Plus
application(s)	microbe id \| susceptibility testing: suitable, microbiological culture: suitable, microbiology: suitable
final pH	6.6±0.2 (25 °C)
Featured Industry	Clinical Testing Food and Beverages Life Science and Biopharma Veterinary
suitability	mycobacteria

Ingredients (g/L)
Casein enzymatic hydrolysate, 1.00
Ammonium sulfate, 0.50
Monopotassium phosphate, 1.50
Disodium phosphate, 1.50
Sodium citrate, 0.40
Magnesium sulfate, 0.05
L-Glutamic acid, 0.50
Ferric ammonium citrate, 0.04
Pyridoxine, 0.001
Biotin, 0.0005
Malachite green, 0.001
Agar, 15.00

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Middlebrook 7H11 Agar Base

Suspend 10.25 g of Middlebrook 7H11 Agar Base in 450 ml of distilled water. Add 2.5 ml of glycerol (Cat. No. 49767). Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 1 vial Middlebrook OADC Growth Supplement (M0678). Mix thoroughly before dispensing.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
product line	BioChemika
form	powder
shelf life	limited shelf life, expiry date on the label
Торговая марка	NutriSelect^® Plus
application(s)	microbe id \| susceptibility testing: suitable, microbiological culture: suitable, microbiology: suitable
final pH	6.6±0.2 (25 °C)
Featured Industry	Clinical Testing Food and Beverages Life Science and Biopharma Veterinary
suitability	mycobacteria

Middlebrook 7H9 Broth Base contains many inorganic salts, which support the growth of mycobacteria. Sodium citrate provides citrate ions in the medium, which retain inorganic cations in the solution.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Middlebrook 7H9 Broth Base

Middlebrook 7H9 Broth Base has been used for the cultivation Mycobacterium bovis bacillus Calmette-Gurin Pasteur strain. It is also recommended for cultivation and sensitivity testing of Mycobacterium tuberculosis.

Ingredients (g/L)
Ammonium sulfate, 0.50
Disodium phosphate, 2.50
Monopotassium phosphate, 1.00
Sodium citrate, 0.10
Magnesium sulfate, 0.05
Calcium chloride, 0.0005
Zinc sulfate, 0.001
Copper sulfate, 0.001
Ferric ammonium citrate, 0.04
L-Glutamic acid, 0.50
Pyridoxine, 0.001
Biotin, 0.0005

Suspend 2.35 g of Middlebrook 7H9 Broth Base in 450 ml of distilled water. Add either 2 ml of glycerol (Cat. No 49767) or 0.5 g of TWEEN® 80 (Cat. No. 93780). Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. Pressure (121°C) for 10 minutes. Cool to 45°C or below and aseptically add 1 vial of Middlebrook ADC Growth Supplement (Cat. No. M0553). Mix well before dispensing.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

sterility	non-sterile
Quality Level	200,
product line	BioChemika
form	powder
shelf life	limited shelf life, expiry date on the label
Торговая марка	NutriSelect^® Plus
application(s)	microbe id \| susceptibility testing: suitable, microbiological culture: suitable, microbiology: suitable
final pH	6.6±0.2 (25 °C)
Featured Industry	Clinical Testing Food and Beverages Life Science and Biopharma Veterinary
suitability	mycobacteria

Ingredients: (per vial)
Bovine albumin fraction V 2.5g
Dextrose 1.0g
Catalase 0.0015g
Distilled water 50 ml

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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Middlebrook ADC Growth Supplement

Reconstitution

Warm up the refrigerated supplement to 45-50°C. Aseptically add the contents of the vial to 450 ml of sterile Middlebrook 7H9 Broth Base (M0178). Mix well and pour into sterile screw capped tubes.

Quality Level	200,
sterility	sterile (Filtered and Aseptic Handled)
form	liquid
shelf life	limited shelf life, expiry date on the label
application(s)	microbiology: suitable
Featured Industry	Clinical Testing Environmental Food and Beverages Life Science and Biopharma Veterinary
storage temp.	2-8°C
suitability	mycobacteria

Middlebrook OADC growth supplement has been used as a supplement in 7H9 liquid medium to culture mycobacteria.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Middlebrook OADC Growth Supplement

Ingredients: (per vial)
Bovine Albumin Fraction V 2.50 g
Dextrose 1.00 g
Catalase 0.002 g
Oleic acid 0.025 g
Sodium chloride 0.425 g
Distilled water 50 mL

Quality Level	200,
sterility	sterile (Filtered and Aseptic Handled)
form	liquid
shelf life	limited shelf life, expiry date on the label
packaging	vial of 50 mL
application(s)	microbiology: suitable
Featured Industry	Clinical Testing Environmental Food and Beverages Life Science and Biopharma Veterinary
storage temp.	2-8°C
suitability	mycobacteria

for the enumeration of microorganisms in milk, milk products, water, ice-cream, etc. by the plate count test

Personal Protective Equipment	Eyeshields,, Gloves,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Milk Agar

Suspend 24 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15 g/L , milk solids (equivalent to 10 ml fresh milk), 1 g/L , peptone, 5 g/L , yeast extract, 3 g/L
Торговая марка	NutriSelect^® Plus
application(s)	bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable, water monitoring: suitable
final pH	7.2±0.2 (25 °C)
Featured Industry	Environmental Food and Beverages
suitability	Micrococcus spp., nonselective for Bacillus spp., nonselective for Candida spp., nonselective for Escherichia coli, nonselective for Pseudomonas spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3

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Milliflex Cassette MXSMCSP48

eCl@ss:	32160801
NACRES:	NA.74

Milliflex® Cassette prefilled with Sabouraud Dextrose Agar is used in Bioburden testing and general microbiological analysis of water especially high purity water (electronics, RO, distilled or purified). It is widely used in pharmaceutical, cosmetic and brewing industry.

United-States Pharmacopeia XXII Microbial Limits Tests

Sabouraud Dextrose w/Chloramphenicol

Target Microorganisms: Yeast and Mold
Yeast produce white, creamy colonies. Mold are rough textured and/or filamentous
QC organisms
C. albicans (ATCC 10231)
S. cerevisiae (ATCC 7754)
E. coli (ATCC 8739) and L. casei (ATCC 393) as negative control

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX OASIS is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	2 to 8 °C, Right side up.
Quality Level	300,
form	plate (pre-poured agar)
shelf life	6 mo.
feature	ready-to-use
packaging	cassette of 10 mL, pack of 48 tests
Торговая марка	Milliflex^®
application(s)	bioburden testing: suitable, microbiology: suitable, water monitoring: suitable
color	clear light amberslightly opalescent
input	sample type pharmaceutical(s) sample type bottled water sample type beer sample type cosmetics
pH	6.4±0.2 (25.0 °C)
Featured Industry	Cosmetics Pharmaceutical
compatibility	for use with Milliflex^®
shipped in	ambient
storage temp.	2-8°C
suitability	molds, yeasts

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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Milliflex Cassette MXSMEND48

eCl@ss:	32160801
NACRES:	NA.74

Milliflex® Cassette are ready to use agar-based media to be used with Milliflex® or Milliflex Oasis® system for convenient, rapid and reliable bioburden testing. The prefilled agar cassette helps to streamline the microbial contamination testing workflow with quality assurance and avoiding risks of exogenous contamination. Each plate is a 3-piece assembly containing approximately 10ml of the media. m-Endo LES Agar is a selective media for detecting total coliforms in drinking water. Sodium lauryl sulphate and sodium desoxycholate are inhibitors of gram-positive bacteria, basic Fuchsin is a pH indicator, sodium sulphite is added to decolorize the basic fuchsin solution and the ethanol additive increases the antibacterial nature of the formulation. Lactose fermenting bacteria produce acetaldehyde that reacts with the sodium sulphite and fuchsin to form red colonies. The development of a metallic sheen occurs when the organism produces aldehydes with the rapid fermentation of lactose. The sheen will be suppressed if the inoculum is too heavy.

Milliflex® Cassette prefilled with m-Endo LES Agar is used in Bioburden testing and general microbiological analysis of water, wastewater and dairy products. It is widely used in pharmaceutical, cosmetic and brewing industry.

m-Endo LES

Coliform colonies appear deep red with distinct green metallic sheen
Target Microorganisms: Coliform species
QC organisms
E. coli (ATCC 25922)
P. aeruginosa (ATCC 9027)
E. aerogenes (ATCC 49701)
S. aureus (ATCC 6538) as negative control

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX OASIS is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	2 to 8 °C, Right side up, protected from light.
Quality Level	300,
form	plate (pre-poured agar)
shelf life	6 mo.
feature	ready-to-use
packaging	cassette of 10 mL, pack of 48 tests
Торговая марка	Milliflex^®
storage condition	protect from light (right side up)
application(s)	bioburden testing: suitable, microbiology: suitable, water monitoring: suitable
color	slightly opalescent rose
input	sample type pharmaceutical(s) sample type cosmetics sample type bottled water sample type beer
pH	7.2±0.2 (25.0 °C)
Featured Industry	Cosmetics Pharmaceutical
compatibility	for use with Milliflex^®
shipped in	ambient
storage temp.	2-8°C
suitability	coliforms

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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Milliflex Cassette MXSMCET48

eCl@ss:	32160801
NACRES:	NA.74

Milliflex® Cassette are ready to use agar-based media to be used with Milliflex® or Milliflex Oasis® system for convenient, rapid and reliable bioburden testing. The prefilled agar cassette helps to streamline the microbial contamination testing workflow with quality assurance and avoiding risks of exogenous contamination. Each plate is a 3-piece assembly containing approximately 10ml of the media. This medium is designed for the isolation and identification of Pseudomonas aeruginosa found in various types of water, especially high purity water. Cetrimide is the selective agent. It does not inhibit the pigment production of P. aeruginosa. In the presence of Cetrimide (which acts as a quaternary ammonium cationic detergent), bacterial cells growth other than P. aeruginosa is inhibited due to the release of nitrogen and phosphorous.

Milliflex® Cassette prefilled with Cetrimide Agar is used in Bioburden testing and general microbiological analysis of water especially high purity water (electronics, RO, distilled or purified). It is widely used in pharmaceutical, cosmetic and brewing industry.

United-States Pharmacopeia, European Pharmacopeia

Cetrimide

QC organisms
P. aeruginosa (ATCC 9027)
P. mirabilis (ATCC 12453) as negative control
P. aeruginosa species appear as green to blue colonies with fluorescence under UV wavelength
Target Microorganisms: Pseudomonas aeruginosa

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX OASIS is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	2 to 8 °C, Right side up.
Quality Level	300,
form	plate (pre-poured agar)
shelf life	6 mo.
feature	ready-to-use
packaging	cassette of 10 mL, pack of 48 tests
Торговая марка	Milliflex^®
application(s)	bioburden testing: suitable, microbiology: suitable, water monitoring: suitable
color	light amberslightly opalescent
input	sample type cosmetics sample type pharmaceutical(s) sample type bottled water sample type beer
pH	7.2±0.2 (25.0 °C)
Featured Industry	Cosmetics Pharmaceutical
compatibility	for use with Milliflex^®
shipped in	ambient
storage temp.	2-8°C
suitability	Pseudomonas aeruginosa

WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Milliflex Cassette MXSMPCA48

eCl@ss:	32160801
NACRES:	NA.74

Milliflex® Cassette are ready to use agar-based media to be used with Milliflex® or Milliflex Oasis® system for convenient, rapid and reliable bioburden testing. The prefilled agar cassette helps to streamline the microbial contamination testing workflow with quality assurance and avoiding risks of exogenous contamination. Each plate is a 3-piece assembly containing approximately 10ml of the media. Plate Count Agar is used for obtaining microbial plate counts from water and other materials of sanitary importance, milk and dairy products, foods. The medium contains Pancreatic digest of casein that provides amino acids, nitrogen, carbon, vitamins and minerals for growth of the organism. Yeast extract primarily supplies the B-complex vitamins. Dextrose is the fermentable carbohydrate and provides an energy source for the growth of bacteria.

Milliflex® Cassette prefilled with Plate Count Agar is used in Bioburden testing and general microbiological analysis of water, wastewater and dairy products. It is widely used in pharmaceutical, cosmetic and brewing industry.

Standard Methods for the Examination of Water and Wastewater, United-States Pharmacopeia

Plate Count

Target Microorganisms: Total aerobic microorganisms

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX OASIS is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	2 to 8 °C, Right side up.
Quality Level	300,
form	plate (pre-poured agar)
shelf life	9 mo.
feature	ready-to-use
packaging	cassette of 10 mL, pack of 48 tests
Торговая марка	Milliflex^®
application(s)	bioburden testing: suitable, microbiology: suitable, water monitoring: suitable
color	clear light amberslightly opalescent
input	sample type beer sample type pharmaceutical(s) sample type cosmetics sample type bottled water
pH	7.0±0.2 (25.0 °C)
Featured Industry	Cosmetics Pharmaceutical
compatibility	for use with Milliflex^®
shipped in	ambient
storage temp.	2-8°C
suitability	aerobic bacteria (total)

WGK Germany	nwg
Flash Point F	Not applicable
Flash Point C	Not applicable

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Milliflex Cassette MXSMCMC24

eCl@ss:	32160801
NACRES:	NA.74

Milliflex® Cassette are ready to use agar-based media to be used with Milliflex® or Milliflex Oasis® system for convenient, rapid and reliable bioburden testing. The prefilled agar cassette helps to streamline the microbial contamination testing workflow with quality assurance and avoiding risks of exogenous contamination. Each plate is a 3-piece assembly containing approximately 10ml of the media. MacConkey Agar is a selective medium for isolation of coliforms and E. coli. Bile salts and crystal violet present in the media largely inhibit the growth of the gram-positive microbial flora. Lactose and the pH indicator neutral red are used to detect lactose degradation. It is useful in differentiating between the lactose fermenting and non-lactose fermenting bacteria and also aid in isolation of gram-negative bacteria.

Milliflex® Cassette prefilled with MacConkey Agar is used in Bioburden testing water monitoring. and general microbiological analysis. It is widely used in pharmaceutical and cosmetic industry

24 ea in 10 mL Agar in plastic Cassette

Standard Methods for the Examination of Water and Wastewater, United-States Pharmacopeia, European Pharmacopeia

MacConkey

Lactose fermenting organisms will appear red with or without a zone of precipitated bile. Non-lactose fermenters will appear as colorless to white colonies. Crystal violet and bile salts inhibits Gram-positive microorganisms, especially Enterococci and Staphylococci
Target Microorganisms: Lactose fermenting bacteria and coliforms
QC organisms
E. coli (ATCC 25922)
P. mirabilis (ATCC 12453)
S. aureus (ATCC 6538) as negative control

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX OASIS is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	2 to 8 °C, Right side up.
Quality Level	300,
form	plate (pre-poured agar)
shelf life	12 mo.
feature	ready-to-use
packaging	cassette of 10 mL, pack of 24 tests
Торговая марка	Milliflex^®
application(s)	bioburden testing: suitable, microbiology: suitable, water monitoring: suitable
color	clear dark red
input	sample type cosmetics sample type beer sample type bottled water sample type pharmaceutical(s)
pH	7.1±0.2 (25.0 °C)
Featured Industry	Cosmetics Pharmaceutical
compatibility	for use with Milliflex^®
shipped in	ambient
storage temp.	2-8°C
suitability	coliforms, lactose-fermenting bacteria

WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Milliflex Cassette MXSMKFS48

eCl@ss:	32160801
NACRES:	NA.74

Milliflex® Cassette are ready to use agar-based media to be used with Milliflex® or Milliflex Oasis® system for convenient, rapid and reliable bioburden testing. The prefilled agar cassette helps to streamline the microbial contamination testing workflow with quality assurance and avoiding risks of exogenous contamination. Each plate is a 3-piece assembly containing approximately 10ml of the media. KF Strep Agar is designed for the recovery of Enterococci (fecal streptococci) found in various types of water. Maltose and lactose serve as a source of energy and sodium azide is the selective agent. Bromocresol purple (indicator dye) turns to yellow when acid is produced and TTC is a redox indicator causing enterococci colonies to have a deep red colour as a result of tetrazolium reduction to an acidic azo dye.

Milliflex® Cassette prefilled with KF Strep Agar is used in Bioburden testing and general microbiological analysis of water, wastewater and dairy products. It is widely used in pharmaceutical, cosmetic and brewing industry.

United-States Pharmacopeia XXII Microbial Limits Tests

KF Streptococcus

Target Microorganisms: Fecal streptococci
QC organisms
S. faecalis (ATCC 29212)
E. coli (ATCC 8739) and E. aerogenes (ATCC 49701) as negative control
Streptococcal colonies appear red or pink surrounded by a yellow zone

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX OASIS is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	2 to 8 °C, Right side up.
Quality Level	300,
form	plate (pre-poured agar)
shelf life	6 mo.
feature	ready-to-use
packaging	cassette of 10 mL, pack of 48 tests
Торговая марка	Milliflex^®
application(s)	bioburden testing: suitable, microbiology: suitable, water monitoring: suitable
color	clear purpleslightly opalescent
input	sample type bottled water sample type pharmaceutical(s) sample type beer sample type cosmetics
pH	7.2±0.2 (25.0 °C)
Featured Industry	Cosmetics Pharmaceutical
compatibility	for use with Milliflex^®
shipped in	wet ice
storage temp.	2-8°C
suitability	fecal bacteria: Streptococcus spp.

WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Milliflex Cassette MXSMTLP48

eCl@ss:	32160801
NACRES:	NA.74

Milliflex® Cassette prefilled with Tryptic Soy Agar is used in Bioburden testing and general microbiological analysis. It is commonly used in determining the efficiency of the sanitation of containers, equipment and surfaces as well as for water miscible cosmetic products. It is widely used in pharmaceutical, cosmetic and brewing industry.

Trypticase Soy w/Poly 80 and Lecithin

Target Microorganisms: Heterotrophic microorganisms
Colonies appear clear to creamy white, some may produce pigment
QC organisms
E. coli (ATCC 25922)
S. aureus (ATCC 6538)

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX OASIS is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	2 to 8 °C, Right side up.
Quality Level	300,
form	plate (pre-poured agar)
shelf life	12 mo.
feature	ready-to-use
packaging	cassette of 10 mL, pack of 48 tests
Торговая марка	Milliflex^®
application(s)	bioburden testing: suitable, microbiology: suitable, water monitoring: suitable
color	clear tan to light amber
input	sample type bottled water sample type pharmaceutical(s) sample type cosmetics sample type beer
pH	7.3±0.2 (25.0 °C)
Featured Industry	Cosmetics Pharmaceutical
compatibility	for use with Milliflex^®
shipped in	ambient
storage temp.	2-8°C
suitability	heterotrophic bacteria

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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Milliflex Cassette MXSMHPC48

eCl@ss:	32160801
NACRES:	NA.74

Milliflex® Cassette are ready to use agar-based media to be used with Milliflex® or Milliflex Oasis® system for convenient, rapid and reliable bioburden testing. The prefilled agar cassette helps to streamline the microbial contamination testing workflow with quality assurance and avoiding risks of exogenous contamination. Each plate is a 3-piece assembly containing approximately 10ml of the media. Heterotrophic Plate Count, formerly known as the standard plate, is a procedure for estimating the number of live heterotrophic bacteria in potable water. Peptic digest of animal tissue is the source of nutrients for organisms, which are not highly fastidious. Gelatin is utilized by microorganisms through a proteolytic mechanism and the addition of glycerol to the basal medium provides a source of carbon and energy.

Milliflex® Cassette prefilled with Heterotrophic Plate Count Agar is used in Bioburden testing and general microbiological analysis of water especially high purity water (electronics, RO, distilled or purified). It is widely used in pharmaceutical, cosmetic and brewing industry.

Heterotrophic Plate Count

Target Microorganisms: Heterotrophic microorganisms
Colonies appear clear to creamy white, some may produce pigment
QC organisms
E. coli (ATCC 25922)
P. aeruginosa (ATCC 9027)
S. aureus (ATCC 6538)
B. subtilis (ATCC 6633)

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX OASIS is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	2 to 8 °C, Right side up.
Quality Level	300,
form	plate (pre-poured agar)
shelf life	6 mo.
feature	ready-to-use
packaging	cassette of 10 mL, pack of 48 tests
Торговая марка	Milliflex^®
application(s)	bioburden testing: suitable, microbiology: suitable, water monitoring: suitable
color	clear tan to light amber
input	sample type beer sample type cosmetics sample type bottled water sample type pharmaceutical(s)
pH	7.1±0.2 (25.0 °C)
Featured Industry	Cosmetics Pharmaceutical
compatibility	for use with Milliflex^®
shipped in	ambient
storage temp.	2-8°C
suitability	heterotrophic bacteria

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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Milliflex Cassette MXSMPIA48

eCl@ss:	32160801
NACRES:	NA.74

Milliflex® Cassette are ready to use agar-based media to be used with Milliflex® or Milliflex Oasis® system for convenient, rapid and reliable bioburden testing. The prefilled agar cassette helps to streamline the microbial contamination testing workflow with quality assurance and avoiding risks of exogenous contamination. Each plate is a 3-piece assembly containing approximately 10ml of the media. Pseudomonas Isolation Agar is a selective medium for isolating Pseudomonas species from water samples. It is also differential media for P. aeruginosa by allowing formation of soluble blue-green pyocyanin pigment. Irgasan, an antimicrobial agent, selectively inhibits gram-positive and gram-negative bacteria other than Pseudomonas sp. and glycerol, magnesium chloride and potassium sulfate promote production of pyocyanin.

Milliflex® Cassette prefilled with Pseudomonas Isolation Agar is used in Bioburden testing and general microbiological analysis of water especially high purity water (electronics, RO, distilled or purified). It is widely used in pharmaceutical, cosmetic and brewing industry.

Pseudomonas Isolation

P. aeruginosa species appear as green to blue colonies with fluorescence under UV wavelength
Target Microorganisms: Pseudomonas species
QC organisms
P. aeruginosa (ATCC 9027)
P. mirabilis (ATCC 12453)

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX OASIS is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	2 to 8 °C, Right side up.
Quality Level	300,
form	plate (pre-poured agar)
shelf life	6 mo.
feature	ready-to-use
packaging	cassette of 10 mL, pack of 48 tests
Торговая марка	Milliflex^®
application(s)	bioburden testing: suitable, microbiology: suitable, water monitoring: suitable
color	light tan to amber
input	sample type beer sample type pharmaceutical(s) sample type bottled water sample type cosmetics
pH	7.0±0.2 (25.0 °C)
Featured Industry	Cosmetics Pharmaceutical
compatibility	for use with Milliflex^®
shipped in	ambient
storage temp.	2-8°C
suitability	Pseudomonas spp.

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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MILLIFLEX OASIS® R2A low nutrient media plates MMSMCRA48

MILLIFLEX OASIS® R2A low nutrient media plates are ready-to-use agar-based media for convenient, rapid and reliable testing. These unique blue colour coded plates offer the control of critical handling steps all along the workflow in conjugation with the MILLIFLEX OASIS® system. They are manufactured and QC tested according to pharmaceutical industry requirements. The plates can be stacked and locked to ensure safe transportation and avoid accidental opening. The empty funnel tray can also be used as an incubation rack. If left unopened, the plates remain sealed unlike standard petri plates which reduces condensation and dehydration for an extended storage time owing to its long shelf life.

MILLIFLEX OASIS® R2A low nutrient media plates are used for the recovery of stressed heterotrophic plate count bacteria found in various types of waters, especially high purity water, Beer and Bottled Water. They are widely used in pharmaceutical and cosmetic industry.
Physical form
48 ready-to-use pre-poured agar plates
Analysis Note
The Colonies appear clear to creamy white, some may produce pigment

MILLIFLEX OASIS is a registered trademark of Merck KGaA, Darmstadt, Germany

material	blue polystyrene plate
Quality Level	300,
Agency/Method	complies with EP, complies with JP, complies with USP
shelf life	12 mo.
packaging	pk of 48 tests
application(s)	bioburden testing: suitable, water monitoring: suitable
pH	7.2±0.2 (25 °C)
Featured Industry	Cosmetics Pharmaceutical
compatibility	for use with MILLIFLEX OASIS^® system
storage temp.	2-8°C
suitability	Aspergillus brasiliensis (ATCC 16404), Bacillus subtilis (ATCC 6633), Candida albicans (ATCC 10231), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 9027), Staphylococcus aureus (ATCC 6538), heterotrophic bacteria (in stressed environment)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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MILLIFLEX OASIS® Sabouraud Dextrose Media Plate MMSMCSD48

MILLIFLEX OASIS® sabouraud dextrose media plates are ready-to-use agar-based media for convenient, rapid and reliable testing. These unique pink colour coded plates offer the control of critical handling steps all along the workflow in conjugation with the MILLIFLEX OASIS® system. They are manufactured and QC tested according to pharmaceutical industry requirements. The plates can be stacked and locked to ensure safe transportation and avoid accidental opening. The empty funnel tray can also be used as an incubation rack. If left unopened, the plates remain sealed unlike standard petri plates which reduces condensation and dehydration for an extended storage time owing to its long shelf life.

MILLIFLEX OASIS® sabouraud dextrose media plates are designed for the recovery of a broad range of yeasts and molds found in various types of water, especially high purity water in the pharmaceutical industry, Beer and Bottled Water They are widely used in pharmaceutical and cosmetic industry.
Physical form
48 ready-to-use pre-poured agar plates
Analysis Note
Yeast produce white, creamy colonies. Mold are rough textured and/or filamentous. Bacteria capable of growth produce clear to white colonies

MILLIFLEX OASIS is a registered trademark of Merck KGaA, Darmstadt, Germany

material	pink polystyrene plate
Quality Level	300,
Agency/Method	complies with EP, complies with JP, complies with USP
shelf life	12 mo.
packaging	pk of 48 tests
application(s)	bioburden testing: suitable, water monitoring: suitable
pH	5.6±0.2 (25 °C)
Featured Industry	Cosmetics Pharmaceutical
compatibility	for use with MILLIFLEX OASIS^® system
storage temp.	2-8°C
suitability	Aspergillus brasiliensis (ATCC 16404), Candida albicans (ATCC 10231), molds, yeasts