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Fluid D STBMRFD34
eCl@ss: 32014001 NACRES: NA.74 General descriptionFluid D is suitable for testing samples that contain lecithin or oil, and compatible with most antibiotics. Composition of this fluid comply with the European Pharmacopeia (EP) 2.6.1, United States Pharmacopeia (USP) 71 and Japanese Pharmacopeia (JP) 4.06. This is a clear colorless fluid and the pH of the product is 7.1 ± 0.2 at 25°C. It can be stored at 2-25°C (protected from light) for 17 months. One box of product contains 4 bottles of 300 mL of fluid. Each bottle has a screw cap with a septum to minimize the risks of cross-contamination and optimize the disinfection procedures.ApplicationSterility testing
Bioburden testing
Fluid D can be used in combination with the STERITEST® sterility testing system for the rinsing of solutions containing oil or lecithin during sterility testing by membrane filtration. It can also be used for the removal of antimicrobial activity by membrane filtration for non-sterile products. The fluid is also excellent for rinsing sterile pathways of devices, and typically used for rinse method testing of medical devices.Culture MediumFluid DLegal InformationSTERITEST is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Room Temperature (2 to 25 °C) Quality Level 400 Agency/Method in accordance with EP 2.6.1, in accordance with JP 4.06, in accordance with USP 71 sterility sterile; autoclaved form liquid shelf life 17 mo. feature ready-to-use packaging pkg of 300 mL (Bottle with screw cap and septum), pack of 4 bottles application(s) sterility testing: suitable volume size 300 mL color clear, with no precipitate and free of visible particles input sample type pharmaceutical(s) pH 7.1±0.2 (25.0 °C) Featured Industry Pharmaceutical compatibility for use with Steritest® shipped in ambient storage temp. 2-30°C suitability nonselective for
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable 172,73 € / 16 952 ₽Получить предложение -
Fluid K STBMRFK34
eCl@ss: 32014001 NACRES: NA.74 General descriptionFluid K is suitable for testing specimens that contain petrolatum, oils, or oily solutions. The fluids composition complies with the European Pharmacopeia (EP) 2.6.1, United States Pharmacopeia (USP) 71 and Japanese Pharmacopeia (JP) 4.06. This is a light-yellow liquid with a pH of 6.9 ± 0.2 at 25°C. It can be stored at 15-25°C (protected from light) for 12 months. A single box contains 4 bottles of 300 mL of fluid, each bottle with a screw cap with septum closure that reduces the risk of cross-contamination and optimizes the disinfection procedures.ApplicationSterility testing
Bioburden testing
Fluid K is used as diluting or rinsing fluid for sterility testing on samples containing petrolatum/oil. It can be used in combination with the STERITEST® sterility testing system and in bioburden testing to rinse membranes and dilute/dissolve samples. The fluid can also be excellent for rinsing pathways of medical devices, and for samples that are difficult to filter or dissolve.ComponentsScrew cap with septumCulture MediumFluid KLegal InformationSTERITEST is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Room Temperature (2 to 25 °C) Quality Level 400, Agency/Method in accordance with EP 2.6.1, in accordance with JP 4.06, in accordance with USP 71 sterility sterile; autoclaved form liquid shelf life 12 mo. feature ready-to-use packaging pkg of 300 mL (Bottle with screw cap and septum), pack of 4 bottles application(s) sterility testing: suitable volume size 300 mL color light, with no precipitate and free of visible particles input sample type pharmaceutical(s) pH 6.9±0.2 (25.0 °C) Featured Industry Pharmaceutical compatibility for use with Steritest® shipped in ambient storage temp. 2-30°C suitability nonselective for
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable 172,73 € / 16 952 ₽Получить предложение -
Fluid Thioglycollate Medium STBMFTM12
eCl@ss: 32014001 NACRES: NA.74 General descriptionFluid Thioglycollate Medium (FTM) is a critical component of our STERITEST® solution. Composition and growth performance of this medium comply with the European Pharmacopeia (EP) 2.6.1, United States Pharmacopeia (USP) 71 and Japanese Pharmacopeia (JP) 4.06. Our manufacturing approach ensures the highest level of quality for our media, improving accuracy and significantly reducing the risk of incorrect interpretation and false results. The appearance of the fluid is light-yellow, slightly opalescent and viscous liquid with a pink ring in suspension 1 cm. The pH of the product is 7.1 ± 0.2 and can be stored at 2-25°C. The incubation temperature varies between 30-35°C, with an incubation time of 7-14 days. The product is supplied as 12 bottles of 100 mL of medium, screw cap with septum closure that minimizes the risks of cross-contamination and optimizes the disinfection procedures.ApplicationFluid Thioglycollate Medium (FTM) is primarily used for the detection of anaerobic bacteria. However, it also enables aerobic bacterial detection. The QC organisms include Clostridium sporogenes (ATCC 19404; ATCC 11437), Staphylococcus aureus (ATCC 6538) and Pseudomonas aeruginosa (ATCC 9027). This medium is used for sterility testing by membrane filtration or direct inoculation.
Sterility testing (membrane filtration and direct inoculation)
Bioburden testingComponentsScrew cap with septumCulture MediumFluid ThioglycollateAnalysis NoteTurbidity indicates growthLegal InformationSTERITEST is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Room Temperature (2 to 25 °C) Quality Level 400, Agency/Method in accordance with EP 2.6.1, in accordance with JP 4.06, in accordance with USP 71 sterility sterile; autoclaved form liquid shelf life 12 mo. feature ready-to-use packaging pkg of 100 mL, pack of 12 bottles application(s) sterility testing: suitable volume size 100 mL input sample type pharmaceutical(s) pH 7.1±0.2 (25 °C) Featured Industry Pharmaceutical compatibility for use with Steritest® shipped in ambient storage temp. 2-30°C suitability anaerobic bacteria, nonselective for
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable 159,78 € / 15 681 ₽Получить предложение -
Fluid Thioglycollate Medium, Double packed STBMFTM12DP
eCl@ss: 32014001 NACRES: NA.74 General descriptionFluid Thioglycollate Medium (FTM) is a critical component of our STERITEST® solution. The product has been formulated and tested to meet the requirements of the EP 2.6.1, USP 71, and JP 4.06. Our manufacturing approach ensures the highest level of quality for our media, improves accuracy and significantly reduces the risk of incorrect interpretation and false results. The sterilized double Tyvek® packaging allows to streamline the cleaning procedures, to minimize the risk of cross-contamination in laminar flow hoods and to secure an efficient decontamination of isolator chambers. The fluid appears light yellow, slightly opalescent and viscous with a pink ring in suspension 1 cm. The pH of the product is 7.1 ± 0.2 and can be stored at 2-25°C. The incubation temperature varies between 30-35°C, with an incubation time of 7-14 days. The product is supplied as 12 bottles of 100 mL of medium, screw cap with septum closure that reduces the risk of cross-contamination and growth inhibition.ApplicationFluid Thioglycollate Medium (FTM) is primarily used for the detection of anaerobic bacteria. However, it also enables aerobic bacterial detection. The QC organisms include Clostridium sporogenes (ATCC 19404), Staphylococcus aureus (ATCC 6538) and Pseudomonas aeruginosa (ATCC 9027). This medium is used for sterility testing by membrane filtration or direct inoculation.
Sterility testing (membrane filtration and direct inoculation)
Bioburden testingComponentsScrew cap with septumCulture MediumFluid ThioglycollateAnalysis NoteTurbidity indicates growthLegal InformationSTERITEST is a registered trademark of Merck KGaA, Darmstadt, Germany
Tyvek is a registered trademark of E. I. du Pont de Nemours and CompanyПараметрыstorage conditions Room Temperature (2 to 25 °C) Quality Level 400, Agency/Method in accordance with EP 2.6.1, in accordance with JP 4.06, in accordance with USP 71 sterility sterile; ethylene oxide treated form liquid feature ready-to-use packaging pkg of 100 mL, pack of 12 bottles application(s) sterility testing: suitable volume size 100 mL input sample type pharmaceutical(s) pH 7.1±0.2 (25 °C) Featured Industry Pharmaceutical compatibility for use with Steritest® shipped in ambient storage temp. 2-30°C suitability anaerobic bacteria
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorocult® DEV Lactose Peptone Broth
Кат. номер 4313-VAR 4313-500G ApplicationThis medium is particularly suited for the enrichment and determination of the titre of coliform bacteria in the bacteriological analysis of water. E. coli shows a positive fluorescence under UV light (366 nm). A positive indole reaction is made for confirmation.Preparation NoteSuspend 36.1 g/litre or 72.2 g/litre, dispense into test tubes fitted with DURHAM tubes, autoclave (15 min at 121° C).FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationFLUOROCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыsterility non-sterile Quality Level 100, form powder shelf life limited shelf life, expiry date on the label composition bromocresol purple, 0.02 g/L , lactose, 10.0 g/L , 4-methylumbelliferyl--D-glucuronidase, 0.1 g/L , peptone from casein, 17.0 g/L , peptone from soya, 3.0 g/L , sodium chloride, 5.0 g/L , tryptophan, 1.0 g/L Торговая марка FluoroCult®, NutriSelect® Basic application(s) microbiology: suitable (microbial identification), water monitoring: suitable final pH 7.2±0.2 (25 °C) Featured Industry Environmental
Food and Beveragessuitability Escherichia coli, coliforms
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Folic acid assay medium
eCl@ss: 34058014 NACRES: NA.85
Кат. номер F5422-100G F5422-VAR ApplicationRecommended for the microbiological assay of folic acid using Streptococcus faecium as the test organism.ComponentsComponents (g/L)
Casein Acid Hydrolysate, Vitamin Free 12.00
Dextrose 40.00
Sodium Citrate 20.00
L-Cystine 0.20
DL-Tryptophan 0.20
Adenine Sulfate 0.02
Guanine Hydrochloride 0.02
Uracil 0.02
Thiamine Hydrochloride 0.002
Pyridoxine Hydrochloride 0.004
Riboflavin (Vitamin B2) 0.002
Nacin 0.002
p-Aminobenzoic Acid (PABA) 0.0002
Biotin 0.0000008
Calcium Pantothenate 0.0004
Dipotassium Phosphate 1.00
Monopotassium Phosphate 1.00
Magnesium Sulfate 0.40
Sodium Chloride 0.02
Ferrous Sulphate 0.02
Manganese Sulphate 0.02Preparation NoteSuspend 7.5 g of Folic Acid Assay Medium in 100 ml of distilled water. Boil to dissolve the medium completely. Mix well to distribute the slight precipitate evenly. For assay, dispense 5 ml of medium per assay tube, and add 5 ml of distilled water to each tube. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes. Cool immediately.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыsterility non-sterile Quality Level 100, form powder shelf life limited shelf life, expiry date on the label Торговая марка NutriSelect® Plus application(s) microbiological culture: suitable, microbiology: suitable pH 6.8±0.2 (25 °C) Featured Industry Food and Beverages
Pharmaceuticalstorage temp. 2-8°C suitability nonselective for Streptococcus spp.
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fraser Broth, Base
NACRES: NA.85
Кат. номер 69198-500G 69198-VAR ApplicationFraser Broth Base with added supplement is recommended by ISO Committee for primary and secondary enrichment medium, for the isolation and enumeration of Listeria monocytogenes from food and animal feedsPreparation NoteSuspend 57.35 grams of dehydrated medium in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Fraser Selective Supplement (Cat. No. 18038) and 2 vials of Fraser Supplement (Cat. No. 90836) to 1000 ml medium for primary enrichment or 1 vial of each to 500 ml medium for secondary enrichment. Mix well and dispense as desired.
Warning : Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin wash with plenty of water immediately.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыsterility non-sterile Quality Level 200, form powder shelf life limited shelf life, expiry date on the label composition casein enzymic hydrolysate, 5.0 g/L , disodium phosphate dihydrate, 12.0 g/L , esculin, 1.0 g/L , lithium chloride, 3.0 g/L , meat extract, 5.0 g/L , peptic digest of animal tissue, 5.0 g/L , potassium dihydrogen phosphate, 1.35 g/L , sodium chloride, 20.0 g/L , yeast extract, 5.0 g/L Торговая марка NutriSelect® Plus application(s) microbiological culture: suitable, microbiology: suitable, pathogen testing: suitable, sample preparation: suitable final pH 7.2±0.2 (25 °C) Featured Industry Agriculture
Environmental
Food and Beveragessuitability selective and differential for Listeria spp.
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
FRASER Listeria Ammonium iron (III) Supplement
Empirical Formula (Hill Notation): C6H8O7 · xFe · xH3N CAS Number: 1185-57-5 MDL number: MFCD03099410 EC Index Number: 214-686-6
Кат. номер 1000920010 1000928001 1.00092.0010 General descriptionFor preparation of FRASER broth, two different supplements are needed: FRASER Listeria Selective Supplement and FRASER Listeria Ammonium Iron (III) Supplement. The ammonium iron (III) citrate promotes the growth of the Listeria spp., and together with esculin (a component of the FRASER broth base), permits the -D-glucosidase detection in Listeria spp.ApplicationFRASER Listeria Ammonium iron (III) Supplement is an additive for the preparation of Half FRASER (Demi FRASER) Broth (Base) or FRASER Broth (Base) for the enrichment of Listeria spp.LinkageReplaces: 1000920017
Analysis Note
Growth promotion test according to ISO 11133
Mixed inoculum of Listeria, E.coli and E.faecalis
Additionaly monoculture of E.coli and E.faecalis
Pre-enrichment in Half Fraser Broth, afterwards enrichment in Fraser Broth
Confirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
10 - 100
- Listeria monocytogenes ATCC 13932 (WDCM 00021): 10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013): 1000
- Escherichia coli ATCC 8739 (WDCM 00012): 1000
- Enterococcus faecalis ATCC 19433 (WDCM 00009): 1000
- Enterococcus faecalis ATCC 29212 (WDCM 00087): 1000
Blacking
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
+
- Listeria monocytogenes ATCC 13932 (WDCM 00021): +
- Escherichia coli ATCC 25922 (WDCM 00013): -
- Escherichia coli ATCC 8739 (WDCM 00012): -
- Enterococcus faecalis ATCC 19433 (WDCM 00009): -
- Enterococcus faecalis ATCC 29212 (WDCM 00087): -
Growth on ALOA medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
>10 CFU
- Listeria monocytogenes ATCC 13932 (WDCM 00021): >10 CFU
Colonies
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
bluegreen with non transparent zone
- Listeria monocytogenes ATCC 13932 (WDCM 00021): bluegreen with non transparent zone
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): <100 CFU
- Enterococcus faecalis ATCC 19433 (WDCM 00009): <100 CFU
Incubation:
Half Fraser Broth: 24 hours at 30°C, aerobic
Fraser Broth: 48 hours at 37°C, aerobic
Confirmation on ALOA-Medium: 48 hours at 37°C, aerobic
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobicLegal InformationALOA is a registered trademark of Biolife Italiana S.r.l.
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method ISO 11290","ISO Quality Level 200, form lyophilized powder potency >2000 mg/kg LD50, oral (Rat) composition Ammonium iron (III) citrate, 250 mg per vial packaging pkg of 10 vials application(s) microbiological culture: suitable","microbiology: suitable","pathogen testing: suitable pH 6-8 (20 °C, 100 g/L in H2O) solubility 1200 g/L Featured Industry Food and Beverages compatibility GranuCult® FRASER Broth (Base) (1.10398)"," GranuCult® Half FRASER (Demi FRASER) Broth (Base) (1.00025) storage temp. 15-25°C suitability Listeria spp. InChI 1S/C6H8O7.Fe.H3N/c7-3(8)1-6(13,5(11)12)2-4(9)10;;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);;1H3/q;+3;/p-3 InChI key FRHBOQMZUOWXQL-UHFFFAOYSA-K Узнать цену -
FRASER Listeria Selective Supplement
Кат. номер 1000938001 1000930010 1.00093.0010 General descriptionFRASER Listeria Selective Supplement is a mixture of acriflavine and nalidixic acid in lyophilized form. It inhibits the growth of accompanying bacteria by selective enrichment of Listeria spp.ApplicationFRASER Listeria Selective Supplement is an additive for the preparation of Half FRASER (Demi FRASER) Broth (Base) or FRASER Broth (Base) for the enrichment of Listeria spp.LinkageReplaces: 1.00093.0017Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +2°C to +8°C. Agency/Method ISO 11290","ISO Quality Level 200, form lyophilized powder composition Acriflavine, 6.25 mg per vial ","Nalidixic acid, 5 mg per vial packaging pkg of 10 vials application(s) microbiological culture: suitable","microbiology: suitable","pathogen testing: suitable pH 8.1-8.6 (20 °C in H2O, Aqueous solution) Featured Industry Food and Beverages compatibility GranuCult® FRASER Broth (Base) (1.10398)"," GranuCult® Half FRASER (Demi FRASER) Broth (Base) (1.00025) storage temp. 2-8°C suitability Listeria spp. Узнать цену -
Fraser Selective Supplement
NACRES: NA.85
Кат. номер 18038-VAR 18038-1VL 18038-5VL General descriptionRecommended by ISO Committee for Listeria monocytogens from food, animal feed etc.Applicationfor Listeria monocytogens from food, animal feed etc.Components(per vial, sufficient for 500 ml medium)
Nalidixic acid 10.0 mg
Acriflavin Hydrochloride 12.5 mgПараметрыAgency/Method according to GB 4789.30-2016 Quality Level 100, sterility sterile form powder shelf life limited shelf life, expiry date on the label application(s) microbiology: suitable, pathogen testing: suitable Featured Industry Environmental
Food and Beveragessuitability Listeria spp.
Safety Informationpictograms GHS05,GHS07,GHS08,GHS09 signalword Danger hcodes H302 - H318 - H361d - H411 pcodes P201 - P273 - P280 - P301 + P312 + P330 - P305 + P351 + P338 + P310 - P308 + P313 Personal Protective Equipment dust mask type N95 (US),, Eyeshields,, Faceshields,, Gloves, RIDADR UN 3077 9 / PGIII WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fraser Supplement
CAS Number: 1185-57-5 EC Number: 214-686-6 MDL number: MFCD03099410
Кат. номер 90836-VAR 90836-1VL 90836-10VL General descriptionRecommended by ISO Committee for selective isolation and enumeration of Listeria monocytogenes from food, animal feeds etc.Applicationfor selective isolation and enumeration of Listeria monocytogenes from food, animal feeds etc.Components(per vial, sufficient for 500 ml medium)
Ferric ammonium citrate 250 mgПараметрыsterility sterile Quality Level 100, form powder shelf life limited shelf life, expiry date on the label application(s) microbiology: suitable, pathogen testing: suitable Featured Industry Environmental
Food and Beveragessuitability Listeria spp. InChI 1S/C6H8O7.Fe.H3N/c7-3(8)1-6(13,5(11)12)2-4(9)10;;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);;1H3/q;+3;/p-3 InChI key FRHBOQMZUOWXQL-UHFFFAOYSA-K
Safety InformationRIDADR UN 3077 9 / PGIII WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Frey mycoplasma broth base
NACRES: NA.85
Кат. номер F6797-500G F6797-VAR ApplicationUsed for cultivation of Avian Mycoplasma.
Frey mycoplasma broth base may be used as a culture medium for examining various proteins of Mycoplasma pulmonis.ComponentsIngredients (g/L)
Casein enzymic hydrolysate, 7.50
Papaic digest of soyabean meal, 2.50
Yeast extract, 5.00
Sodium chloride, 5.00
Potassium chloride, 0.40
Magnesium sulphate, 0.20
Disodium phosphate, 1.60
Monopotassium phosphate, 0.10Preparation NoteSuspend 22.3 g of Frey Mycoplasma Broth Base in 900 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 100 ml of sterile heat inactivated horse serum (H1138). Mix well before dispensing.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыsterility non-sterile Quality Level 100, form powder shelf life limited shelf life, expiry date on the label Торговая марка NutriSelect® Plus application(s) microbiological culture: suitable, microbiology: suitable final pH 7.7±0.2 (25 °C) Featured Industry Clinical Testing
Food and Beverages
Life Science and Biopharma
Veterinarysuitability mycoplasma
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Узнать цену -
Gassner Agar
NACRES: NA.74
Кат. номер 48716-VAR-F 48716-500G-F 48716-100G-F ApplicationSelective agar for the detection and isolation of pathogenic Enterobacteriaceae in food and other material according to Gassner.Preparation NoteDissolve 76.87 g in 1 litre distilled water. Autoclave at 121°C for 15 minutes.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыsterility non-sterile Quality Level 100, form powder shelf life limited shelf life, expiry date on the label composition agar, 13 g/L , lactose, 43 g/L , metachrome yellow, 1.25 g/L , peptone, 14 g/L , sodium chloride, 5 g/L , water blue, 0.62 g/L Торговая марка NutriSelect® Basic application(s) bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable final pH 7.2±0.2 (25 °C) Featured Industry Agriculture
Environmental
Food and Beveragessuitability selective and differential for Escherichia coli, selective and differential for coliforms, selective and differential for enterobacteriaceae
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Gelatin from porcine skin
CAS Number: 9000-70-8 MDL number: MFCD00081638
Кат. номер 73865-VAR 73865-500-G 73865-500G ComponentsGelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.Параметрыquality controlled endotoxin level, medium gel strength Quality Level 100, shelf life limited shelf life, expiry date on the label application(s) microbiology: suitable impurities 2.0% sulfated ash, 5 EU/mg endotoxins loss 15% loss on drying pH 4.0-6.0 gel strength 150-210 g Bloom solubility H2O: 0.67 g/10 mL at 50 °C (colorless to very light yellow, clear to very hazy) anion traces chloride (Cl-): 3000 mg/kg cation traces Ca: 2000 mg/kg, Cd: 5 mg/kg, Co: 5 mg/kg, Cr: 10 mg/kg, Cu: 50 mg/kg, Fe: 50 mg/kg, K: 500 mg/kg, Mg: 500 mg/kg, Mn: 5 mg/kg, Na: 5000 mg/kg, Ni: 5 mg/kg, Pb: 5 mg/kg, Zn: 10 mg/kg
Safety InformationWGK Germany nwg Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Glucose Bromcresol Purple Agar
NACRES: NA.85
Кат. номер 16447-VAR 16447-500G 16447-7L ApplicationGlucose Bromcresol Purple Agar is used for isolation and enumeration of Enterobacteriaceae and Bacillus cereus.Preparation NoteSuspend 41.52 g in 1000 ml distilled water. Heat to boiling with gentle swirling to dissolve the agar completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45-50°C and pour into sterile petri plates.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыAgency/Method according to ISO 21258-1:2017 Quality Level 200, sterility non-sterile product line BioChemika form powder shelf life limited shelf life, expiry date on the label composition Agar, 15.0 g/L , Brom cresol purple, 0.015 g/L , Casein enzymic hydrolysate, 10.0 g/L , Glucose, 10.0 g/L , Sodium chloride, 5.0 g/L , Yeast extract, 1.5 g/L Торговая марка NutriSelect® Plus application(s) microbe id | utilization test: suitable, microbiology: suitable (microbial identification) final pH 7.0±0.2 (25 °C) Featured Industry Agriculture
Clinical Testing
Environmental
Food and Beveragessuitability Citrobacter spp., Neisseria spp., Proteus spp., Serratia spp., Shigella spp., Streptococcus spp., nonselective and differential for Bacillus cereus, nonselective and differential for Bacillus spp., nonselective and differential for Enterobacter spp., nonselective and differential for Escherichia coli, nonselective and differential for Klebsiella spp., nonselective and differential for Salmonella spp., nonselective and differential for coliforms, nonselective and differential for enterobacteriaceae
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
GranuCult® Baird-Parker agar (base)
Кат. номер 1054060500 1054065000 1.05406.0500 1054060507 General descriptionGranuCult® Baird-Parker agar (base) is used for the isolation and enumeration of Staphylococcus aureus. This medium contains lithium chloride and tellurite to inhibit the growth of accompanying microbial flora, whereas pyruvate and glycine selectively stimulate the growth of staphylococci. Agar is the solidifying agent. The egg-yolk content makes the medium opaque. Staphylococcus colonies show two characteristic features when grown in this medium: (a) characteristic zones and rings are formed as a result of lipolysis and proteolysis, and (b) reduction of tellurite to tellurium produces a black coloration. The egg-yolk reaction and tellurite reduction usually occur together with a positive coagulase reaction and thus can serve as an index for the latter. According to EN ISO 6888-1, the addition of sulfamethazine is advised to suppress the growth and swarming of Proteus sp., if these are suspected in the test sample.ApplicationGranuCult® Baird-Parker agar (base) is used for the isolation, presumptive identification, and enumeration of Staphylococcus aureus from food and animal feed as well as from other materials.LinkageReplaces: 1.05406.5007Analysis NoteAppearance (clearness): clear to turbid
Appearance (color): yellowish-brown
pH-value (25°C): 6.6 - 7.0
Solidification behaviour (2hrs., 45°C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)):
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)):
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): 50 %
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): 50 %
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): no growth
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): no growth
Growth (Staphylococcus saprophyticus ATCC 15305 (WDCM 00159)): no limit
Growth (Staphylococcus epidermidis ATCC 12228 (WDCM 00036)): no limit
Growth (Enterococcus hirae ATCC 8043 (WDCM 00089)): no limit
Growth (Proteus mirabilis ATCC 29906 (WDCM 00023)): no limit
Colonies (Staphylococcus aureus ATCC 6538 (WDCM 00032)): Black or grey colonies with clear halo
Colonies (Staphylococcus aureus ATCC 25923 (WDCM 00034)): Black or grey colonies with clear halo
Colonies (Staphylococcus saprophyticus ATCC 15305 (WDCM 00159)): Black or grey colonies without clear halo
Colonies (Staphylococcus epidermidis ATCC 12228 (WDCM 00036)): Black or grey colonies without clear halo
Colonies (Enterococcus hirae ATCC 8043 (WDCM 00086)): Black or grey colonies without clear halo
Colonies (Proteus mirabilis ATCC 29906 (WDCM 00023)): Brown or black colonies without clear halo
Incubation:
24 ± 2 to 48 ± 2 hours at 37 ± 1 °C aerobic
E.coli 48 ± 2 hours at 37 ± 1 °C aerobic
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy AgarFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method APHA, ISO 6888 Quality Level 200, reg. compliance FDA (BAM) form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbe id | specific enzyme detection: suitable, microbiological culture: suitable, microbiology: suitable pH 6.8 (37 °C, 58 g/L in H2O) solubility 58 g/L bulk density 570 kg/m3 Featured Industry Food and Beverages storage temp. 15-25°C suitability Staphylococcus spp. Узнать цену -
GranuCult® BAT agar
Кат. номер 1079940500 1.07994.0500 US1107994/0500 General descriptionGranuCult® BAT agar supports the growth of Alicyclobacillus spp. These are aerobic, Gram-positive spore-forming bacteria, with optimum growth at low pH and increased temperatures. Alicyclobacilli are spoilage organisms, especially affecting the quality of fruit juices.
GranuCult® BAT agar contains glucose as a carbon and energy source. Yeast extract is the source of vitamins, particularly the B-group. Potassium dihydrogen phosphate acts as a buffering system. The low pH and the high incubation temperature inhibit the contaminating flora. This medium contains many trace elements, which supply the specific requirements of the Alicyclobacillus spp. agar is the solidifying agent however, the gel strength is weak due to low pH.ApplicationGranuCult® BAT agar is suitable for the enumeration, detection, and identification of Alicyclobacillus spp. from fruit juices, other beverages, and their ingredients, environmental samples, including process water and other materials in the area of food production and food handling.Analysis NoteAppearance (clearness): slightly turbid to turbid
Appearance (color): yellowish
pH-value (25°C): 3.9 - 4.1
Growth (Alicyclobacillus acidocaldarius DSMZ 446): +
Growth (Alicyclobacillus acidoterrestris DSMZ 2498): +
Growth (Alicyclobacillus cycloheptanicus DSMZ 4006): +
Growth (Alicyclobacillus hesperidium DSMZ 12766): +
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): -
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): -
Incubation: A.acidocaldarius 72 hrs.; 60 °C aerobic; other strains 72 hrs.; 45 °C.
Reference for Staph.aureus und E.coli blood agar (Incubation: 24 hrs.; 35 °C).FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method according to IFU 12 Quality Level 200, form medium granules (dehydrated (DCM)) packaging pkg of 500 g Торговая марка GranuCult® storage condition protect from light (both UV and Sun light) application(s) microbiological culture: suitable, microbiology: suitable Featured Industry Food and Beverages storage temp. 15-25°C suitability Alicyclobacillus spp. (spp.) Узнать цену -
GranuCult® BAT Broth 1079930500
General descriptionBAT broth is suitable for the enrichment of spore-forming thermo-acidophilic spoilage bacteria (Alicyclobacillus spp.). It contains glucose as a carbon and energy source. Yeast extract is a source for vitamins, particularly the B-group. Potassium dihydrogen phosphate acts as a buffering system. The low pH value and the high incubation temperature inhibit the contaminating flora. This medium contains many trace elements, which supply the specific requirements for the growth of Alicyclobacillus spp.ApplicationBAT broth is used for the enrichment of Alicyclobacillus spp. from juice and juice-related products and their ingredients, environmental samples, including process water, and other materials in the area of food production and food handling.Features and BenefitsThe formulation of this BAT broth is according to the revision of IFU Method No. 12 of 2019, and is less toxic due to the removal of boric acid and cobalt(II) chloride hexahydrateFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 200, Agency/Method according to IFU Method No. 12 sterility non-sterile form medium granules (dehydrated culture medium (DCM)) Торговая марка GranuCult® prime application(s) bioburden testing: suitable Featured Industry Food and Beverages suitability Alicyclobacillus spp. (spp) Узнать цену -
GranuCult® BPLS agar, modified
Кат. номер 1107470500 1.10747.0500 General descriptionGranuCult® BPLS agar (modified) contains Brilliant green that inhibits the growth of the accompanying Gram-positive microbial flora, Salmonella typhi, and Shigella. However, the lower concentration of brilliant green in the modified BPLS agar does not strongly inhibit other Salmonella spp. The medium contains both lactose and sucrose. The color change (red to yellow) in the pH indicator Phenol red indicates the degradation of lactose to acid. Salmonellae do not ferment either lactose or sucrose and appear as red colonies surrounded by a bright red zone. Furthermore, in contrast to BPL agar, the sucrose in the medium allows identification of accompanying, weakly lactose-positive or lactose-negative, but sucrose-positive microorganisms.ApplicationGranuCult® BPLS agar (modified) is a selective agar suitable for the isolation of salmonellae (except for S. typhi) from meat, meat products and other foodstuffs.Analysis NoteAppearance (clearness): clear
Appearance (colour): red
pH-value (25°C): 6.7 - 7.1
Recovery on test medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): 40 %
Recovery on test medium (Salmonella choleraesuis ATCC 13312): 40 %
Recovery on test medium (Salmonella enteritidis NCTC 5188 (WDCM 00119)): 40 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Recovery on test medium (Proteus hauseri ATCC 13315):
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): 0.01 %
Recovery on test medium (Enterococcus faecalis ATCC 33186): 0.01 %
Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003)): 0.01 %
Colony colour (Salmonella typhimurium ATCC 14028 (WDCM 00031)): pink-red
Colony colour (Salmonella choleraesuis ATCC 13312): pink-red
Colony colour (Salmonella enteritidis NCTC 5188 (WDCM 00119)): pink-red
Colony colour (Escherichia coli ATCC 25922 (WDCM 00013)): yellow
Colony colour (Proteus hauseri ATCC 13315): yellow
Incubation: 24 hrs.; 35 °C; aerobic.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. form medium granules (dehydrated (DCM)) Quality Level 200, packaging pkg of 500 g Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 6.8-7.0 (37 °C, 52 g/L in H2O, after autoclaving) solubility 52 g/L bulk density 650 kg/m3 Featured Industry Food and Beverages storage temp. 15-25°C suitability Salmonella spp. (spp.) Узнать цену -
GranuCult® Brain Heart Infusion broth
Кат. номер 1104930500 1.10493.0500 General descriptionBHI (Brain Heart Infusion) broth is a granulated selective medium recommended for the cultivation of various fastidious bacteria and the cultivation of Staphylococci for the plasma coagulase test. The granules ensure rapid and uniform dissolution in water. It also prevents clumping of the medium and inhalation of the airborne powder. Enzymatic digest of animal tissues and infusions (calf brain and beef heart) serve as sources of carbon, nitrogen, essential growth factors, amino acids, and vitamins. Glucose serves as a source of energy. Disodium phosphate helps in maintaining the buffering action of the medium, whereas sodium chloride maintains the osmotic equilibrium of the medium.ApplicationBHI (Brain Heart Infusion) broth is a highly nutritious and buffered medium to support the growth of fastidious and non-fastidious microorganisms, including aerobic and anaerobic bacteria.Analysis NoteAppearance (colour): brown
Appearance (clearness): clear
pH-value (25°C): 7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): 100
Inoculum on reference medium (Streptococcus pneumoniae ATCC 6305): 100
Inoculum on reference medium (Streptococcus pyogenes ATCC 19615): 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): 100
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)): 100
Inoculum on reference medium (Bacteroides fragilis ATCC 25285): 100
Inoculum on reference medium (Haemophilus influenzae ATCC 33391): 100
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): good to very good
Growth (Streptococcus pneumoniae ATCC 6305): good to very good
Growth (Streptococcus pyogenes ATCC 19615): good to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): good to very good
Growth (Candida albicans ATCC 10231 (WDCM 00054)): good to very good
Growth (Bacteroides fragilis ATCC 25285): good to very good
Growth (Haemophilus influenzae ATCC 33391): good to very good
Incubation:
24 ± 2 hours at 37 ± 1 °C aerobic
C.albicans 44 ± 4 hours at 37 ± 1 °C aerobic
B.fragilis 44 ± 4 hours at 37 ± 1 °C anaerobic
H.influenza 44 ± 4 hours at 37 ± 1 °C microaerophilic and with addition of X+V-factor.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method APHA, ISO 6888 Quality Level 200, form medium granules (dehydrated (DCM)) application(s) microbiological culture: suitable, microbiology: suitable, pathogen testing: suitable pH 7.2-7.6 (37 °C, 37 g/L in H2O, after autoclaving) solubility 37 g/L bulk density 730 kg/m3 Featured Industry Food and Beverages storage temp. 15-25°C suitability heterotrophic bacteria Узнать цену -
GranuCult® Buffered Listeria Enrichment Broth (base)
Кат. номер 1096280500 1096285007 1096285000 1.09628.0500 General descriptionGranuCult® Buffered Listeria Enrichment Broth (base) is a modification of the formulation of Tryptic Soy Broth (CASO) with the addition of 6 g/L yeast extract and by increasing its buffering strength. Dextrose is the carbohydrate source. Sodium chloride maintains the osmotic balance of the medium. Phosphate acts as a buffer. Sodium pyruvate mediates the recovery of sub-lethally damaged Listeria spp. The addition of acriflavine, cycloheximide, and nalidixic acid inhibits the growth of the accompanying flora.ApplicationGranuCult® Buffered Listeria Enrichment Broth (base) is suitable for the selective enrichment of Listeria spp.LinkageReplaces: 1.09628.5007Analysis NoteAppearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25°C): 7.1 - 7.5
Inoculum on reference medium (Listeria monocytogenes ATCC 19114 (WDCM 00110)):
Inoculum on reference medium (Listeria monocytogenes ATCC 19111 (WDCM 00020)):
Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)):
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)):
Colony count (Listeria monocytogenes ATCC 19114 (WDCM 00110)): 1E+08 CFU/ml
Colony count (Listeria monocytogenes ATCC 19111 (WDCM 00020)): 1E+08 CFU/ml
Colony count (Listeria innocua ATCC 33090 (WDCM 00017)): 1E+08 CFU/ml
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): no growth
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): passes test
Incubation 48 h at 30 °CFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. reg. compliance FDA (BAM) Quality Level 200, form medium granules (dehydrated (DCM)) packaging pkg of 500 g Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable, pathogen testing: suitable pH 7.1-7.5 (25 °C in H2O) Featured Industry Food and Beverages storage temp. 15-25°C suitability Listeria spp. (spp.) Узнать цену -
GranuCult® Buffered Peptone Water
Кат. номер 1072280500 1072285000 1072289025 1072289027 1072284590 1072289010 1072288060 1.07228.5000 1.07228.0500 General descriptionBuffered Peptone Water is rich in nutrients and produces high resuscitation rates for sub lethally injured bacteria and intense growth. The phosphate buffer system prevents bacterial damage caused by changes in the pH of the medium. Peptone including enzymatic digest of casein acts as a source of carbon, nitrogen, vitamins and minerals whilst sodium chloride maintains the osmotic balance.
GranuCult® is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.ApplicationBuffered Peptone Water is used in the testing of food and water samples. This product is suitable for the preliminary non selective enrichment of bacteria, particularly pathogenic Enterobacteriaceae such as Salmonella and Cronobacter, from food, animal feed, water, and other materials. The product is also suitable as a diluent for general use.LinkageReplaces: 1072285007; 1.07228Analysis NoteAppearance (clearness): clear
Appearance (colour): yellowish
pH-value (25°C): 6.8 - 7.2
Growth promotion test in accordance with the current version of DIN EN ISO 11133 and the harmonised method of EP, USP and JP.
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): 100
Inoculum on reference medium (Salmonella Abony NCTC 6017): 100
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)): good to very good
Growth (Salmonella Abony NCTC 6017): good to very good
Incubation:
18 ± 2 hours at 37 ± 1 °C
S.abony 18 - 24 hours at 30 - 35 °CFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method APHA, EP, ISO 19250, ISO 21528-1, ISO 22964, ISO 6579-1, ISO 6887 Quality Level 200, reg. compliance FDA (BAM) form medium granules (dehydrated (DCM)) Торговая марка GranuCult® prime application(s) microbiological culture: suitable, microbiology: suitable, pathogen testing: suitable pH 7.2 (37 °C, 25 g/L in H2O, after autoclaving) solubility 25.5 g/L bulk density 800 kg/m3 Featured Industry Food and Beverages storage temp. 15-25°C suitability Cronobacter spp., Salmonella spp., enterobacteriaceae Узнать цену -
GranuCult® Casein-peptone lecithin polysorbate broth (base)
Кат. номер 1117230500500 1117230500 1.11723.0500 US11117230000 General descriptionCasein-peptone lecithin polysorbate broth (base) is a granulated medium recommended for general microbiological analysis. The granules ensure rapid and uniform dissolution in water, prevent clumping of the medium, and inhalation of the airborne powder. Casein enzymic hydrolysate provides the nitrogen, vitamins, amino acids, and carbon in the broth base. Casein peptone in the medium present optimal conditions for the spore germination and regeneration of the damaged microorganisms. Lecithin and polysorbate 20 inactivate many antimicrobial compounds.ApplicationCasein-peptone lecithin polysorbate broth (base) is recommended for diluting samples of pharmaceutical, cosmetic, and other raw materials or final products for determining the total microbial counts.Analysis NoteAppearance (clearness): clear to almost clear
Appearance (colour): yellowish
pH-value (25°C): 6.9 - 7.3
Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032)): +
Growth (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)): +
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003)): +
Growth (Candida albicans ATCC 10231 (WDCM 00054)): +
Growth (Aspergillus brasiliensis (formerly A. niger) ATCC 16404(WDCM 00053)): +
Incubation: 24 hrs.; 35 °C; aerobic;
Aspergillus und C.albicans up to 3 days; 20 - 25 °C; aerobic .FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Quality Level 200, Agency/Method USP form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable Featured Industry Cosmetics
Pharmaceuticalstorage temp. 15-25°C Узнать цену -
Granucult® Enterobacteriaceae Enrichment Mossel broth
Кат. номер 1053940500 1053945000 1.05394.0500 1.05394.5000 General descriptionGranucult® Enterobacteriaceae Enrichment Mossel broth is intended for the selective enrichment of Enterobacteriaceae. The undesired accompanying bacterial flora is inhibited by brilliant green and ox bile. Glucose favors the growth of all Enterobacteriaceae, and the high buffering capacity of the culture medium prevents the formed acid from killing the culture. Glucose is the main energy source and supports the rapid growth of all Enterobacteriaceae, while enzymatic digest of animal tissue provides nitrogen, vitamins, and amino acids.ApplicationGranucult® Enterobacteriaceae Enrichment Mossel broth is intended for the selective enrichment of Enterobacteriaceae from food and animal feed and other materials.Analysis NoteAppearance (clearness): clear
Appearance (color): green
pH-value (25°C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum E.coli resp. Salmonella and E.faecalis.
Additionaly monoculture of E.faecalis
Confirmation on VRBD agar resp. Tryptic soy agar
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): 100
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): 1000
Growth on VRBD-Agar(Escherichia coli ATCC 8739 (WDCM 00012)): >10 colonies
Growth on VRBD-Agar(Escherichia coli ATCC 25922 (WDCM 00013)): >10 colonies
Growth on VRBD-Agar(Salmonella typhimurium ATCC 14028 (WDCM 00031)): >10 colonies
Growth on VRBD-Agar(Salmonella enteritidis ATCC 13076 (WDCM 00030)): >10 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Colonies (Escherichia coli ATCC 8739 (WDCM 00012)): pink to red colonies with or without precipitation halo
Colonies (Escherichia coli ATCC 25922 (WDCM 00013)): pink to red colonies with or without precipitation halo
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)): pink to red colonies with or without precipitation halo
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)): pink to red colonies with or without precipitation halo
Incubation:
EE broth: 24 ± 2 hours at 37 ± 1 °C aerobic
Confirmation on VRBD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobicFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method ISO 21528 Quality Level 200, form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 7.3 (37 °C, 45 g/L in H2O, after autoclaving) solubility 45 g/L bulk density 630 kg/m3 Featured Industry Food and Beverages storage temp. 15-25°C suitability enterobacteriaceae Узнать цену -
GranuCult® FRASER broth (base)
Кат. номер 1103985007 1103985000 1103980500 1.10398.0500 General descriptionGranuCult® FRASER broth (base) has high nutrient content and a large buffering capacity that creates optimum growth conditions for Listeria spp. Lithium chloride, nalidixic acid, and acriflavine hydrochloride inhibit the growth of accompanying bacteria. The addition of aesculin and ammonium iron (III) citrate facilitates the detection of the -D-glucosidase activity by forming a black complex of aesculin with iron (III) ions. A black coloration can develop during incubation, but this reaction is not exclusive to Listeria spp. and cultures that show no blackening cannot be assumed to be free of Listeria spp. Hence, every primary and secondary enrichment in Fraser broth must be sub-cultured on selective plating media. From GranuCult® FRASER Broth (Base), the primary selective enrichment Half Fraser broth, as well as the secondary enrichment Fraser broth can be prepared by addition of Ammonium iron (III) citrate supplement (1.00092,) and Selective Supplement (1.00093,).ApplicationGranuCult® FRASER broth (base) is suitable for the primary and secondary selective enrichment of Listeria spp. from food and animal feed as well as from environmental samples and other materials.LinkageReplaces: 1.10398Analysis NoteAppearance (clearness): clear to almost clear
Appearance (color): yellowish-brown
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Listeria, E.coli and E.faecalis
Additionaly monoculture of E.coli and E.faecalis
Confirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): 100
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): 1000
Blacking (Listeria monocytogenes ATCC 13932 (WDCM 00021)): +
Blacking (Listeria monocytogenes ATCC 35152 (WDCM 00109)): +
Blacking (Escherichia coli ATCC 25922 (WDCM 00013)): -
Blacking (Escherichia coli ATCC 8739 (WDCM 00012)): -
Blacking (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Blacking (Enterococcus faecalis ATCC 29212 (WDCM 00087)): -
Growth on ALOA medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): >10 colonies
Growth on ALOA medium(Listeria monocytogenes ATCC 35152 (WDCM 00109)): >10 colonies
Colonies (Listeria monocytogenes ATCC 13932 (WDCM 00021)): blue green colonies with opaque halo
Colonies(Listeria monocytogenes ATCC 35152 (WDCM 00109)): blue green colonies with opaque halo
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): <100 colonien
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): <100 colonies
Incubation:
Half-Fraser Broth: 24 ± 2 hours at 30 ± 1°C
Fraser Broth: 48 ± 2 hours at 37 ± 1°C
Confirmation on ALOA medium: 44 ± 4 hours at 37 ±1°C
Confirmation on Tryptic soy agar: 24 ± 3 hours at 37 ± 1°CFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationALOA is a registered trademark of Biolife Italiana S.r.l.
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method APHA, according to ISO 11290 Quality Level 200, form medium granules (dehydrated (DCM)) packaging pkg of 500 g Торговая марка GranuCult® storage condition protect from light (both UV and Sunlight) application(s) microbiological culture: suitable, microbiology: suitable, pathogen testing: suitable pH 7.0-7.4 (25 °C, 57.4 g/L in H2O) solubility 57.4 g/L Featured Industry Food and Beverages storage temp. 15-25°C suitability selective for Listeria spp. Узнать цену -
GranuCult® GIOLITTI-CANTONI broth (Base)
Кат. номер 1106750500 1.10675.0500 US1110675/0500 General descriptionGranuCult® GIOLITTI-CANTONI broth (Base) promotes the growth of staphylococci due to the presence of pyruvate, glycine, Tween® 80, and a high concentration of mannitol. This medium stimulates the growth of injured staphylococci and aids detection of the organism when present in small numbers only. Lithium chloride inhibits the Gram-negative contaminants, while potassium tellurite and glycine inhibit the Gram-positive contaminants. Furthermore, anaerobiosis suppresses the micrococci to a certain degree. Black coloration or black precipitate of the culture medium, due to reduction of tellurite to metallic tellurium, indicates the growth of staphylococci.ApplicationGranuCult® GIOLITTI-CANTONI broth (Base) is suitable for the enumeration and detection of coagulase-positive staphylococci (Staphylococcus aureus and other species) from food and animal feeding stuff as well as from environmental samples in food production and food handling.Analysis NoteAppearance (clearness): clear
Appearance (color): yellowish-brown
pH-value (25°C): 6.7 - 7.1
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of S.aureus and E.coli.
Additionaly monoculture of E.coli.
Confirmation on Baird-Parker-Agar resp. Tryptic soy agar
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): 100
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): 10000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): 10000
Growth on Baird-Parker-Agar(Staphylococcus aureus ATCC 6538 (WDCM 00032)): >10 colonies
Growth on Baird-Parker-Agar(Staphylococcus aureus ATCC 25923 (WDCM 00034)): >10 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Colonies (Staphylococcus aureus ATCC 25923 (WDCM 00034)): Black or grey colonies with clear halo
Colonies (Staphylococcus aureus ATCC 6538 (WDCM 00032)): Black or grey colonies with clear halo
Incubation
24 ± 2 hours at 37 ± 1 °C aerobic.
E.coli 48 ± 2 hours at 37 ± 1 °C aerobic.
Confirmation on Baird-Parker-Agar: 48 ± 2 hours at 37 ± 1 °C aerobic.
Confirmation on Tryptiy Soy-Agar: 24 ± 2 hours at 37 ± 1 °C aerobic.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLCПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method ISO 6888 Quality Level 200, form medium granules (dehydrated (DCM)) packaging pkg of 500 g Торговая марка GranuCult® storage condition protect from light (both UV and Sunlight) application(s) microbiological culture: suitable, microbiology: suitable pH 6.8-7.0 (20 °C, 54 g/L in H2O, after autoclaving) solubility 54 g/L bulk density 500 kg/m3 Featured Industry Food and Beverages storage temp. 15-25°C suitability Staphylococcus spp. (spp.)
Safety Informationpictograms GHS07 signalword Warning hcodes H317 - H319 pcodes P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338 WGK Germany WGK 3 Узнать цену -
GranuCult® Half FRASER broth (base) with antibiotics
Кат. номер 1000259017 1000250507 1000255007 1000255000 1000259010 1000250500 1000251430 1.00025.0500 General descriptionGranuCult® Half FRASER broth (base) with antibiotics has high nutrient content and a large buffering capacity that creates optimum growth conditions for Listeria spp. Lithium chloride, nalidixic acid, and acriflavine hydrochloride inhibit the growth of accompanying bacteria. The detection of the -D-glucosidase activity is possible by adding aesculin and ammonium iron (III) citrate, forming a black complex of aesculin with iron (III) ions. A black coloration can develop during incubation, but this reaction is not exclusive to Listeria spp. Hence, according to EN ISO 11290-1, every primary and secondary enrichment in Fraser broth must be sub-cultured on selective plating media. From TM="GranuCult"] Half FRASER (Demi FRASER) Broth (Base) with Antibiotics primary selective enrichment Half Fraser broth can be prepared by addition of one vial Ammonium iron (III) citrate supplement (1.00092,) per 500 mL Half Fraser Broth (base). For the secondary enrichment step, Fraser broth can be prepared by adding one additional vial of reconstituted Fraser Listeria selective supplements (1.00093,) per 500 mL Half Fraser Broth.ApplicationGranuCult® Half FRASER broth (base) with antibiotics is suitable for the primary selective enrichment of Listeria spp. from food and animal feed as well as from environmental samples and other materials.Analysis NoteAppearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Listeria, E.coli and E.faecalis
Additionaly monoculture of E.coli and E.faecalis
Afterwards confirmation on ALOA®-Medium resp. Tryptic Soy agar
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): 100
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): 1000
Growth on ALOA medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): >10 colonies
Growth on ALOA medium(Listeria monocytogenes ATCC 35152 (WDCM 00109)): >10 colonies
Colonies (Listeria monocytogenes ATCC 13932 (WDCM 00021)): blue green colonies with opaque halo
Colonies(Listeria monocytogenes ATCC 35152 (WDCM 00109)): blue green colonies with opaque halo
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): <100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): <100 colonies
Incubation:
Half Fraser Broth: 24 ± 2 hours at 30 ± 1°C
Confirmation on ALOA-Medium: 44 ± 4 hours at 37 ± 1°C
Confirmation on Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1°CFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationALOA is a registered trademark of Biolife Italiana S.r.l.
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method APHA, according to ISO 11290 Quality Level 200, form medium granules (dehydrated (DCM)) packaging pkg of 500 g Торговая марка GranuCult® storage condition protect from light (both UV light and sun light) application(s) microbiological culture: suitable, microbiology: suitable, pathogen testing: suitable pH 7.0-7.4 (25 °C, 55 g/L in H2O, after autoclaving) solubility 55 g/L Featured Industry Food and Beverages storage temp. 15-25°C suitability Listeria spp. Узнать цену -
GranuCult® Lactose broth
Кат. номер 1076619027 1076610507 1076612507 1076615000 1076610500 1076612500 1.07661.0500 General descriptionLactose Broth is an inhibitor-free granulated medium for the use in the presumptive testing for coliforms, especially E. coli, and the enrichment of Salmonella. The granules ensure rapid and uniform dissolution in water, it also prevents clumping of the medium and inhalation of the airborne powder. Lactose utilization is indicated by gas production. The gas liberated is collected in Durhams tubes. Peptone present in the medium supply nitrogenous and carbonaceous compounds, long-chain amino acids, and other essential nutrients to the organisms. Lactose is a fermentable carbohydrate for the coliforms.ApplicationLactose Broth is recommended for the performance and confirmation of the presumptive test to detect coliform bacteria in food, water, dairy, and Pharmaceutical products.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method APHA, EP, USP Quality Level 200, reg. compliance FDA (BAM) form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 6.7-7.1 (25 °C, 13 g/L in H2O) bulk density 650 kg/m3 Featured Industry Food and Beverages storage temp. 15-25°C suitability Salmonella spp. Узнать цену -
Granucult® Listeria Enrichment Broth
Кат. номер 1105490500 1105495000 1.10549.0500 1.10549.5000 General descriptionThe Granucult® Listeria Enrichment Broth formulation is a modified Tryptic Soy (CASO) Broth with an additional 6 g/liter of yeast extract. The inhibition of undesired accompanying flora is achieved by the addition of acriflavin HCl, cycloheximide, and nalidixic acid. In contrary to Listeria Enrichment Broth (Base) (1.11951), this broth already contains antibiotic substances.ApplicationGranucult® Listeria Enrichment Broth is intended for the selective enrichment of Listeria.LinkageReplaces: 1.10549.5007Analysis NoteAppearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25°C): 7.1 - 7.5
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): 10 - 100
Inoculum on reference medium (Listeria monocytogenes ATCC 19114 (WDCM 00110)): 10 - 100
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): 10 - 100
Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)): 10 - 100
Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)): 1E+04 CFU/ml
Colony count (Listeria monocytogenes ATCC 19114 (WDCM 00110)): 1E+04 CFU/ml
Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)): 1E+04 CFU/ml
Colony count (Listeria innocua ATCC 33090 (WDCM 00017)): 1E+04 CFU/ml
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)): 1000 CFU/ml
Incubation: 24 hrs.; 30 °C; aerobically.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store below +15°C. reg. compliance FDA IDF-FIL Quality Level 200, form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 7.1-7.5 (30 °C, 36.1 g/L in H2O, after autoclaving) solubility 36.1 g/L Featured Industry Food and Beverages storage temp. 2-30°C suitability Listeria spp. Узнать цену -
GranuCult® Malt extract agar
Кат. номер 1053985000 1053980500 US1105398/5000 1.05398.0500 1.05398.5000 US1105398/0500 General descriptionMalt extract agar is recommended for the detection, isolation, and enumeration of yeasts and molds. The acidic pH of the medium supports the growth of Yeasts and Molds while it suppresses the growth of bacterial flora. The addition of certain indicator dyes to the medium allows differentiation of yeast and bacterial colonies. Furthermore, a modified malt extract agar is recommended for the selective cultivation of Aspergillus flavus.ApplicationMalt extract agar is used for the detection, isolation, and enumeration of fungi, particularly yeasts and molds, in various materials and for the cultivation of test strains for the microbiological vitamin assays.Analysis NoteAppearance (clearness): clear to slightly opalescent
Appearance (color): brown
pH-value (25°C): 5.4 - 5.8
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)):
Inoculum on reference medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Inoculum on reference medium (Saccharomyces cerevisiae ATCC 9080):
Inoculum on reference medium (Rhodotorula mucilaginosa DSM 70403):
Colony count (Candida albicans ATCC 10231 (WDCM 00054)):
Colony count (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Colony count (Saccharomyces cerevisiae ATCC 9080):
Colony count (Rhodotorula mucilaginosa DSM 70403):
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): 70 %
Recovery on test medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): 70 %
Recovery on test medium (Saccharomyces cerevisiae ATCC 9080): 70 %
Recovery on test medium (Rhodotorula mucilaginosa DSM 70403): 70 %
Growth (Geotrichum candidum DSM 1240): good to very good
Growth (Penicillium commune ATCC 10428): good to very good
Growth (Aspergillus brasiliensis (formerly A. niger) ATCC 16404(WDCM 00053)): good to very good
Growth (Trichophyton ajelloi ATCC 28454): moderate to good
Incubation: Molds 7 days at 26 ± 2 °C, Yeasts 3 days, aerobic
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Reference media: SABOURAUD AgarFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method EP, JP, USP Quality Level 200, form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 5.6 (22 °C, 48 g/L in H2O, after autoclaving) solubility 48 g/L bulk density 590 kg/m3 Featured Industry Food and Beverages storage temp. 15-25°C suitability molds, yeasts Узнать цену -
GranuCult® Mannitol egg yolk polymyxin agar (base)
Кат. номер 1052670500 1.05267.0500 US1105267/0500 General descriptionGranuCult® Mannitol egg yolk polymyxin agar (base) is used for the enumeration of Bacillus cereus. This medium contains two indicator systems, mannitol, and phenol red and egg yolk. Enzymatic digest of casein and meat extract provide carbon and nitrogen sources. The selective inhibitory component polymyxin suppresses the growth of many unwanted microorganisms. Bacteria that ferment mannitol to produce acids produce yellow staining of the medium with phenol red as a pH indicator. Bacillus cereus does not ferment mannitol so the medium around the colonies remains unchanged or is discolored due to light alkalization by forming pink colonies. The lecithin present in egg yolk is cleaved by the Bacillus cereus-lecithinase, which leads to the formation of an opalescent white precipitation zone surrounding the pink colonies. Bacillus anthracis, Bacillus mycoides, Bacillus pseudodomycoides, Bacillus thuringiensis, and Bacillus weihenstephanensis may not be distinguishable from Bacillus cereus on this medium.ApplicationGranuCult® Mannitol egg yolk polymyxin agar (base) is used for the enumeration of presumptive Bacillus cereus from food and animal feed as well as from environmental samples and other materials.Analysis NoteAppearance (clearness): clear to slightly opalescent
Appearance (color): red
pH-value (25°C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001)):
Colony count (Bacillus cereus ATCC 11778 (WDCM 00001)):
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)): 50 %
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003)): no limit
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Colonies (Bacillus cereus ATCC 11778 (WDCM 00001)): pink colonies with precipitation halo
Colonies (Bacillus subtilis ATCC 6633 (WDCM 00003)): yellow colonies without precipitation halo
Incubation:
B.cereus 24 ± 3 to 44 ± 4 hours at 30 ± 1 °C
other teststrains 44 ± 4 hours at 30 ± 1 °C
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy AgarFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method APHA, ISO 21871, ISO 7932 Quality Level 200, reg. compliance FDA (BAM) form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 7.0-7.4 (25 °C, 43 g/L in H2O, after autoclaving) solubility 43 g/L Featured Industry Food and Beverages storage temp. 15-25°C suitability Bacillus cereus Узнать цену -
GranuCult® Mannitol salt phenol-red agar
Кат. номер 1054045000 1054040500 1.05404.0500 General descriptionMannitol salt phenol-red agar is a modified version of the selective agar for the isolation and presumptive identification of Staphylococcus aureus. The composition of Mannitol Salt Agar supports the growth of Staphylococcus aureus, whereas the high salt content of 7.5 % inhibit many other microorganisms. Degradation of mannitol to acid correlates with the pathogenicity of S. aureus and thus, serves as an indicator for this species. Some halophilic Enterococci and Vibriones also grow on Mannitol Salt Agar. In contrast to the most other Staphylococci, S. aureus can build acids from mannitol. Therefore, they build up yellow colonies with yellow zones. S. capitis, S. simulans, S. carnosus, S. scuri, S. lentus, and S. gallinarum are also mannitol positive.ApplicationMannitol salt phenol-red agar is used for the isolation and presumptive identification of Staphylococcus aureus in non sterile pharmaceuticals and food.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method EP, JP, USP Quality Level 200, form medium granules feature ready-to-use Торговая марка GranuCult® application(s) bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable pH 7.2-7.6 (25 °C, 108 g/L in H2O, after autoclaving) solubility 108 g/L bulk density 770 kg/m3 Featured Industry Pharmaceutical storage temp. 15-25°C suitability Staphylococcus spp. (pathogenic) Узнать цену -
GranuCult® Modified Tryptic Soy Broth with Novobiocin
Кат. номер 1092050500 1.09205.0500 US1109205/0500 General descriptionModified Tryptic Soy Broth with Novobiocin is recommended for the enrichment of enterohemorrhagic E. coli (EHEC). The nutrients in mTSB broth provide favorable growth conditions, while the mixture of bile salts No. 3 and Novobiocin suppress the growth of the Gram-positive microbial flora.ApplicationmTSB broth with Novobiocin is used for the selective enrichment of enterohemorrhagic E. coli (EHEC) as well as detection of E. coli serotype O157:H7 in foods.Analysis NoteAppearance (clearness): clear
Appearance (color): yellowish-brown
pH-value (25°C): 7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of E.coli and S.aureus
Additionaly monoculture of S.aureus
Inoculum on reference medium (Escherichia coli ATCC 35150 (O157)): 100
Inoculum on reference medium (Escherichia coli ATCC 700728(WDCM 00014)): 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): 100
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): 10000
Growth (Escherichia coli ATCC 35150 (O157)): >10 yellowish-brown colonies on CT-SMAC agar
Growth (Escherichia coli ATCC 700728 (WDCM 00014)): >10 yellowish-brown colonies on CT-SMAC agar
Growth Escherichia coliATCC 25922 (WDCM 00013): >10 pink colonies on CT-SMAC agar
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): total inhibition on TSA
Incubation:
mTSB broth: 18 - 24 hours at 41,5 °C, aerobic
Confirmation on CT-SMAC Agar resp. TSA: 24 ± 3 hours at 37 ± 1 °C aerobicFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method ISO 16654 Quality Level 200, reg. compliance FDA (BAM) description with Novobiocin (20 mg/L) acc. ISO 16654 GranuCult form medium granules (dehydrated (DCM)) composition Novobiocin, 20 mg/L Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable, pathogen testing: suitable pH 7.1-7.5 (25 °C, 33 g/L in H2O, after autoclaving) solubility 33 g/L Featured Industry Food and Beverages storage temp. 15-25°C suitability Escherichia coli (O157:H7 ), selective for Escherichia coli (EHEC), urea-degrading bacteria Узнать цену -
Granucult® Modified UVM broth (Base)
Кат. номер 1108240500 1108245000 1108249025 1.10824.0500 1108249027 General descriptionGranucult® Modified UVM broth (Base) is suitable for the selective enrichment of Listeria in the two-stage procedure. The combination of various peptones, extracts, salts, and buffer substances enables the good growth of Listeria. The selectivity is due to the antiproliferative substances nalidixic acid and acriflavine hydrochloride. The two-stage enrichment method has demonstrated its value, especially with sample materials (meat and meat products) that are characterized by a high level of accompanying flora.ApplicationGranucult® Modified UVM broth (Base) is suitable for the selective enrichment of Listeria in foods and beverages.LinkageReplaces: 1.10824.5007Analysis NoteAppearance (clearness): clear to turbid
Appearance (color): yellowish-brown
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): 100
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): 100
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): 10000
Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001)): 10000
Growth (Listeria monocytogenes ATCC 35152 (WDCM 00109)): >10 colonies on ALOA medium, blue green with opaque halo
Growth (Listeria monocytogenes ATCC 13932 (WDCM 00021)): >10 colonies on ALOA medium, blue green with opaque halo
Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032)): <100 colonies on TSA
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): <100 colonies on TSA
Mixed inoculum of Listeria and S.aureus
Additionaly monoculture of S.aureus and B.cereus
Confirmation on ALOA medium resp. Tryptic soy agar
Incubation: 20 - 26 hours at 30 ± 2°C, aerobic
Confirmation on ALOA medium: 44 ± 4 hours at 37 ±1°C
Confirmation on Tryptic soy agar: 24 ± 3 hours at 37 ± 1°CFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method USDA-FSIS Quality Level 200, form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable, pathogen testing: suitable pH 7.0-7.4 (25 °C, 54.4 g/L in H2O, after autoclaving) solubility 54.4 g/L Featured Industry Food and Beverages storage temp. 15-25°C suitability Listeria spp. Узнать цену -
GranuCult® MULLER-KAUFFMANN Tetrathionate Novobiocin broth (base)
Кат. номер 1058780500 1.05878.0500 US1105878/0500 General descriptionMKTTn (MULLER-KAUFFMANN Tetrathionate Novobiocin) broth is a selective medium suitable for the enrichment and isolation of Salmonella. Tetrathionate in the broth suppresses the growth of coliform and other enteric bacteria. It is produced from thiosulfate by adding iodine to the culture medium. Salmonella, Proteus, and some other species of bacteria can reduce tetrathionate. Calcium carbonate buffers the sulphuric acid, which is generated when tetrathionate is reduced. Bile promotes the growth of Salmonella, but largely inhibits the accompanying bacteria. Brilliant green and novobiocin suppress the growth of primarily Gram-positive bacteria.ApplicationMKTTn (MULLER-KAUFFMANN Tetrathionate Novobiocin) broth (base) is used for the selective enrichment of Salmonella from food, animal feed, and other materials.Analysis NoteAppearance (clearness): turbid with sediment
Appearance (colour): green
pH-value (25 °C): 7.8 - 8.2
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Salmonella, E.coli and P.aeruginosa
Additionaly monoculture of E.coli and E.faecalis
Confirmation on XLD agar resp. Tryptic soy agar
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): 1000
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): 1000
Growth on XLD agar (Salmonella typhimurium ATCC 14028 (WDCM 00031)): >10 colonies
Growth on XLD agar (Salmonella enteritidis ATCC 13076 (WDCM 00030)): >10 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): <100 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): <100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): <10 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): <10 colonies
Incubation:
MKTTn broth: 24 ± 3 hours at 37 ± 1 °C aerobic
Confirmation on XLD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobicFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Quality Level 200, Agency/Method ISO 11133, according to ISO 6579 form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable Featured Industry Food and Beverages storage temp. 15-25°C suitability selective for Salmonella spp. Узнать цену -
GranuCult® Nutrient Broth
Кат. номер 1054430500 1054435000 US11054430000 1054430507 1.05443.0500 General descriptionGranuCult® Nutrient Broth is designed as a basic medium to support the growth of fastidious and non-fastidious microbes in test samples.
The medium is supplemented with peptone and yeast extract contributing to the nitrogen requirements, vitamins and amino acids for the growth of microbes. Sodium Chloride maintains the osmotic balance glucose serves as the energy source.ApplicationGranuCult® Nutrient Broth is designed as a basic medium to support the growth of fastidious and non-fastidious microbes in test samples.LinkageReplaces: 1.05443Analysis NoteAppearance (clearness): clear
Appearance (color): yellowish to yellowish-brown
pH-value (25°C): 6.6 - 7.0
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): 100
Inoculum on reference medium (Streptococcus pyogenes ATCC 12344): 100
Inoculum on reference medium (Listeria monocytogenes ATCC 19118): 100
Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001)): 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): 100
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): fair to very good
Growth (Streptococcus pyogenes ATCC 12344): fair to very good
Growth (Listeria monocytogenes ATCC 19118): fair to very good
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): fair to very good
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): fair to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): fair to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): fair to very good
Incubation: 24 ± 2 hours at 37 ± 1 °C, aerobic
Reference media: Tryptic Soy AgarFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Quality Level 200, sterility sterile; autoclavable form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable pH 7.0 (37 °C, 8 g/L in H2O, after autoclaving) solubility 8 g/L bulk density 470 kg/m3 Featured Industry Food and Beverages
Pharmaceuticalstorage temp. 15-25°C suitability heterotrophic bacteria Узнать цену -
GranuCult® Oxford Agar (Base)
Кат. номер 1070040500 1.07004.0500 General descriptionGranuCult® Oxford Agar (Base) formulation is based on Columbia Agar with the addition of lithium chloride, acriflavin, colistin sulfate, cefotetan, cycloheximide, and fosfomycin. These ingredients suppress the growth of the common bacteria (e.g., Gram-negative bacteria and a greater part of Gram-positive bacteria). Lithium chloride is one of the ingredients of Oxford Agar base, while the other substances are derived from the Oxford Listeria Selective Supplement (1.07006,). Listeria monocytogenes hydrolyses esculin to esculetin and forms a black complex with iron (III) ions, thereby producing brown-green colored colonies with a black halo.ApplicationGranuCult® Oxford Agar (Base) is a selective agar for the isolation and detection of Listeria monocytogenes in milk and milk products and other food products.Analysis NoteBase tested with Oxford-Listeria-selektiv-supplement art. no. 1.07006.
Appearance (clearness): clear
Appearance (color): blueish-brown to greenish-brown
pH-value (25°C): 6.8 - 7.2
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)):
Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Colony count (Listeria innocua ATCC 33090 (WDCM 00017)):
Recovery on test medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): 50
Recovery on test medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): 50
Recovery on test medium (Listeria innocua ATCC 33090 (WDCM 00017)): 50
Growth Escherichia coliATCC 25922 (WDCM 00013): total inhibition
Growth(Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): partial inhibition
Growth (Candida albicans ATCC 10231 (WDCM 00054)): partial inhibition
Black zone (Listeria monocytogenes ATCC 35152 (WDCM 00109)): black zone
Black zone (Listeria monocytogenes ATCC 13932 (WDCM 00021)): black zone
Black zone (Listeria innocua ATCC 33090 (WDCM 00017)): black zone
Incubation: up to 48 hours at 35 ± 1 °C, aerobic
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy AgarFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method ISO 11290 Quality Level 200, reg. compliance according to FDA (BAM) form medium granules (dehydrated (DCM)) packaging pkg of 500 g Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 6.9-7.1 (35 °C, 58.5 g/L in H2O, after autoclaving) solubility 58.5 g/L bulk density 810 kg/m3 Featured Industry Food and Beverages storage temp. 15-25°C suitability Listeria spp. (spp.) Узнать цену -
GranuCult® PALCAM agar (Base)
Кат. номер 1117550500 1.11755.0500 General descriptionGranuCult® PALCAM agar (Base) provides quantitative cultivation of Listeria monocytogenes and inhibits the accompanying Gram-negative and most of the Gram-positive bacteria. Polymyxin, acriflavin, ceftazidime, and lithium chloride result in the selectivity of the medium. L. monocytogenes break down the esculin in the medium to glucose and esculetin. Esculetin forms an olive-green to black complex with iron (III) ions, which stains the colonies of L. monocytogenes. Mannitol-positive accompanying bacteria such as Staphylococci grow as yellow colonies if they are not inhibited.ApplicationGranuCult® PALCAM agar (Base) is suitable for the detection and isolation of Listeria monocytogenes from feces, biological samples, foodstuffs, and heavily contaminated material from the environment.Analysis NoteBase tested with PALCAM-Listeria selective supplement acc. to VAN NETTEN et.al. art. no. 1.12122.
Appearance (clearness): clear to slightly opalescent
Appearance (color): red
pH-value (25°C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)):
Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Colony count (Listeria innocua ATCC 33090 (WDCM 00017)):
Recovery on test medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): 50 %
Recovery on test medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): 50 %
Recovery on test medium (Listeria innocua ATCC 33090 (WDCM 00017)): 50 %
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth(Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): partial inhibition
Black zone (Listeria monocytogenes ATCC 35152 (WDCM 00109)): black zone
Black zone (Listeria monocytogenes ATCC 13932 (WDCM 00021)): black zone
Black zone (Listeria innocua ATCC 33090 (WDCM 00017)): black zone
Incubation: up to 48 hours at 35 ± 1 °C, aerobic
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy AgarFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method ISO 11290 Quality Level 200, reg. compliance according to FDA (BAM) form powder (dehydrated (DCM)) packaging pkg of 500 g Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 6.9-7.1 (25 °C, 68.8 g/L in H2O, after autoclaving) solubility 68.8 g/L bulk density 650 kg/m3 Featured Industry Food and Beverages storage temp. 15-25°C suitability Listeria spp. Узнать цену -
GranuCult® PEMBA (Polymyxin Pyruvate Egg yolk Mannitol Bromothymolblue Agar) (base) 1205890500
General descriptionGranuCult® PEMBA agar is a selective culture medium containing two indicator systems, mannitol and bromothymol blue, and egg yolk. B. cereus does not utilize mannitol and forms precipitates of hydrolyzed lecithin from the egg yolk.
B. cereus grow as turquoise to peacock blue colonies, since the medium is slightly alkalized by its metabolites (bromothymol blue reaction). The yolk reaction (by lecithinase) also leads to a precipitate surrounding the colony, which appears in the color of the agar or similar to the colony. Growth and lecithinase activity are often so strong that the precipitation is already evident before a colony is visible.
Bacillus cereus is identified by colony morphology, color, and egg yolk hydrolysis. Microscopic examination of the cell and spore morphology and the formation of intracellular lipid globules in vegetative cells is a rapid method of confirmation.
Mannitol-positive bacteria form yellow colonies with a yellow zone in the surrounding medium.ApplicationGranuCult® PEMBA agar is used for the selective isolation of viable presumptive Bacillus cereus from Food products and animal feeds and the environmental samples in the area of food production and food handling.PrincipleLow concentration of nutrients and the selective inhibitory component polymyxin help to attain selectivity. Although, some Proteus spp. and Gram-positive cocci also grow in the medium. The low peptone level and the addition of the pyruvate improve the egg yolk precipitation and sporulation. Bromothymol blue acts as a pH indicator to detect mannitol utilization. Enzymatic digest of casein provides carbon and nitrogen sources. Sodium chloride maintains the osmotic balance of the medium, while agar is the solidifying agent.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 200, Agency/Method ISO 21871 sterility non-sterile; Not processed or packaged aseptically form medium granules Торговая марка GranuCult® application(s) pathogen testing: suitable pH range 7.0 - 7.4 (25 °C) Featured Industry Food and Beverages storage temp. 15-25°C suitability selective and differential for bacteria: Bacillus Cereus Узнать цену -
GranuCult® Plate Count agar
Кат. номер 1.05463.0201 1054630500 1054630619 1054630585 1054635000 US11054630000 1.05463.0500 1.05463.5000 US11.05463.0201 General descriptionPlate Count Agar (also called Tryptone Glucose Yeast Agar or Casein-Peptone Dextrose Yeast Agar) is a granulated culture medium. It does not contain any inhibitors or indicators, and it is relatively rich in its nutrients. The enzymatic digest of casein (tryptone) with a high level of free amino acids is a nitrogen source. Yeast extract primarily supplies the B-complex vitamins. Glucose is an energy source for the growth of bacteria, while agar is the solidifying agent. Our granulated culture media offer several advantages, such as superior consistency, faster dissolving, longer shelf-life, safety, and easy handling.ApplicationPlate Count Agar is suitable for the determination of the total microbial content from milk, dairy products, food and animal feed, water, beverages, and other materials.LinkageReplaces: 1.05463.5007FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method APHA, ISO 17410, ISO 4833 Quality Level 200, reg. compliance FDA (BAM) form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 7.0 (30 °C, 22 g/L in H2O, after autoclaving) solubility 22.5 g/L bulk density 560 kg/m3 Featured Industry Food and Beverages storage temp. 15-25°C suitability aerobic bacteria (total)
Safety InformationWGK Germany WGK 3 Узнать цену -
GranuCult® RAPPAPORT-VASSILIADIS-Soya broth (base)
Кат. номер 1077000507 1077000500 1.07700.0507 1.07700.0500 General descriptionRVS (RAPPAPORT-VASSILIADIS-Soya) broth is a selective medium for the enrichment and isolation of Salmonella. It is a modification of the original Rappaport medium, where soya peptone is used instead of tryptone (peptone from casein) to improve recovery rates of Salmonella. The efficiency of RVS broth for selection of Salmonella is based on (a) the ability of Salmonella sp. to multiply at relatively high osmotic pressures, at relatively low pH values, at a high temperature, and with modest nutritional requirements and (b) the suppression of the toxic effect of malachite green towards Salmonellae in the presence of magnesium chloride.ApplicationRVS (RAPPAPORT-VASSILIADIS-Soya) broth (base) is used for the selective enrichment of Salmonella from food and animal feed, water, and other materials.Analysis NoteAppearance (clearness): clear
Appearance (colour): darkblue
pH-value (25 °C): 5.0 - 5.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Salmonella, E.coli and P.aeruginosa
Additionaly monoculture of E.coli and E.faecalis
Confirmation on XLD agar resp. Tryptic soy agar
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): 1000
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): 1000
Growth on XLD agar (Salmonella typhimurium ATCC 14028 (WDCM 00031)): >10 colonies
Growth on XLD agar (Salmonella enteritidis ATCC 13076 (WDCM 00030)): >10 colonies
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)): colourless with black centre
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)): colourless with black centre
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): <100 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): <100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): <10 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): <10 colonies
Incubation:
RVS broth: 24 ± 3 hours at 41.5 ± 1 °C aerobic
Confirmation on XLD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobicFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method ISO 11133, ISO 19250, ISO 6579 Quality Level 100,, 200, form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 5.0-5.4 (25 °C, 42.5 g/L in H2O, after autoclaving) bulk density 700 kg/m3 Featured Industry Food and Beverages storage temp. 15-25°C suitability Salmonella spp. Узнать цену -
GranuCult® SABOURAUD 4% dextrose agar
Кат. номер 1054380500 1054385000 1.05438.0500 1.05438.5000 General descriptionSabouraud 4% Dextrose Agar is a complex, granulated, dehydrated culture medium for cultivation and isolation of yeasts and molds. It also serves as the absence test for Candida albicans. The high concentration of dextrose and the low pH promotes the growth, the formation of spores (Conidia and Sporangia), and the formation of pigments of yeasts and molds. Additionally, it inhibits the growth of bacteria.ApplicationSabouraud 4% Dextrose Agar is designed for the determination of the total count of yeasts and molds in non-sterile products.Analysis NoteAppearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25°C): 5.4 - 5.8
Typical composition (g/litre): Peptone from casein5.0; Peptone from meat 5.0; D(+)Glucose 40.0; Agar-agar 15.0.
Growth (Trichophyton mentagrophytes ATCC 18748): fair to very good
Growth (Trichophyton rubrum ATCC 28188): fair to good
Growth (Trichophyton ajelloi ATCC 28454): fair to good
Growth (Microsporum gallinae ATCC 12108): fair to very good
Growth (Microsporum canis ATCC 36299): good to very good
Growth (Geotrichum candidum DSM 1240): good to very good
Growth (Penicillium commune ATCC 10428): good to very good
Incubation: 7 days; 28°C
Growth promotion test in accordance with the harmonised method of EP, USP and JP.
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)): 10 - 100
Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404(WDCM 00053)): 10 - 100
Colony count (Candida albicans ATCC 10231 (WDCM 00054)):
Colony count (Aspergillus brasiliensis (formerly A. niger) ATCC 16404(WDCM 00053)):
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): 70 %
Recovery on test medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404(WDCM 00053)): 50 %
Incubation: C. albicans and A. brasiliensis up to 5 daysat 20-25°C.
The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method EP 5.6, JP 4.05, USP 29 Quality Level 200, form medium granules Торговая марка GranuCult® application(s) bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable pH 5.6 (25 °C, 65 g/L in H2O, after autoclaving) solubility 65 g/L bulk density 680 kg/m3 Featured Industry Industrial QC
Pharmaceuticalstorage temp. 15-25°C suitability molds, yeasts Узнать цену -
GranuCult® Triple sugar iron agar
Кат. номер 1039150507 1039150500 1.03915.0500 General descriptionGranuCult® Triple sugar iron agar is recommended for the identification of Gram-negative enterobacteria. It contains lactose, sucrose, and glucose as fermentable carbohydrates. The pH indicator, phenol red, indicates the degradation of sugars to produce acids by the varying color of the medium (from red to yellow). Several species of bacteria reduce thiosulfate to hydrogen sulfide that combines with ferric ions of iron salt to produce iron sulfide, an insoluble black precipitate.ApplicationGranuCult® Triple sugar iron agar is suitable for the identification of Enterobacteriaceae in meat, meat products, and milk.Analysis NoteAppearance (clearness): clear
Appearance (colour): red
pH-value (25°C): 7.2 - 7.6
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Citrobacter freundii ATCC 8090): good to very good
Growth (Enterobacter cloacae ATCC 13047 (WDCM 00083)): good to very good
Growth (Shigella flexneri ATCC 12022 (WDCM 00126)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)): good to very good
Growth (Proteus mirabilis ATCC 14153): good to very good
Growth (Proteus hauseri ATCC 13315): good to very good
Butt (Escherichia coli ATCC 25922 (WDCM 00013)): yellow
Butt (Citrobacter freundii ATCC 8090): yellow/black
Butt (Enterobacter cloacae ATCC 13047 (WDCM 00083)): yellow
Butt (Shigella flexneri ATCC 12022 (WDCM 00126)): yellow
Butt (Salmonella typhimurium ATCC 14028 (WDCM 00031)): yellow/black
Butt (Salmonella enteritidis ATCC 13076 (WDCM 00030)): yellow/black
Butt (Proteus mirabilis ATCC 14153): yellow/black
Butt (Proteus hauseri ATCC 13315): yellow/black
Slant surface (Escherichia coli ATCC 25922 (WDCM 00013)): yellow
Slant surface (Citrobacter freundii ATCC 8090): yellow
Slant surface (Enterobacter cloacae ATCC 13047 (WDCM 00083)): yellow
Slant surface (Shigella flexneri ATCC 12022 (WDCM 00126)): red
Slant surface (Salmonella typhimurium ATCC 14028 (WDCM 00031)): red
Slant surface (Salmonella enteritidis ATCC 13076 (WDCM 00030)): red
Slant surface (Proteus mirabilis ATCC 14153): red/black
Slant surface (Proteus hauseri ATCC 13315): yellow
Incubation: 24 hrs.; 35 °C; aerobic.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. form medium granules (dehydrated (DCM)) Quality Level 200, packaging pkg of 500 g Торговая марка GranuCult® application(s) microbe id | utilization test: suitable, microbiological culture: suitable, microbiology: suitable pH 7.4 (37 °C, 65 g/L in H2O, after autoclaving) solubility 65 g/L bulk density 650 kg/m3 Featured Industry Food and Beverages storage temp. 15-25°C suitability Citrobacter spp., Enterobacter spp., Escherichia coli, Klebsiella spp., Proteus spp., Salmonella spp., Shigella spp., coliforms, enterobacteriaceae Узнать цену -
GranuCult® Tryptic Soy agar
Кат. номер 1054580500 1054585000 1.05458.0201 1.05458.5000 1054585007 US11.05458.0201 NACL1054580500/1 NACL1054580500 1.05458.0500 General descriptionTryptic Soy Agar is a granulated, universal culture medium. It is free from inhibitors and indicators and has a wide spectrum of applications. The combination of the two peptones, the enzymatic digest of casein and soybean, provides high nutrition by supplying organic nitrogen, amino acids, and longer-chained peptides. Sodium chloride present in this complex medium provides the osmotic balance, while agar-agar is the solidifying agent.ApplicationTryptic Soy agar is suitable for the isolation and cultivation of a wide range of microorganisms from different materials and is used in quality control of pharmaceutical products.LinkageReplaces: 1054585007FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method APHA, EP, ISO 11133, JP, USP Quality Level 200, reg. compliance FDA (BAM) form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 7.1-7.5 (25 °C, 40 g/L in H2O, after autoclaving) solubility 40 g/L bulk density 620 kg/m3 Featured Industry Pharmaceutical storage temp. 15-25°C suitability aerobic bacteria (total)
Safety Informationpictograms GHS07 signalword Warning hcodes H317 pcodes P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 WGK Germany WGK 3 Узнать цену -
GranuCult® Tryptose sulfite cycloserine agar (base)
Кат. номер 1119720500 1.11972.0500 US1111972/0500 General descriptionGranuCult® Tryptose sulfite cycloserine agar (base) is intended for the isolation and differentiation of Clostridium perfringens. The superior nutrient base provides optimal conditions for the development of Clostridia. Colonies producing hydrogen sulfide are characterized by blackening due to the reaction with sulfite and iron salt. Cycloserine inhibits the accompanying bacterial flora and may cause the colonies that develop to remain smaller. It also reduces diffusion and thus the disturbing blackening around the Clostridium perfringens colonies. TSC agar with MUP contains a fluorogenic substrate for the alkaline and acid phosphatase in addition to cycloserine 4-Methylumbelliferylphosphate (MUP). The acid phosphatase is a high specific indicator for Clostridium perfringens. It splits the fluorogenic substrate MUP, forming 4-methylumbelliferone, which can be identified by its fluorescence in long wave UV light. Thus, a strong suggestion for the presence of Clostridium perfringens can be obtained.ApplicationGranuCult® Tryptose sulfite cycloserine agar (base) is intended for the isolation and differentiation of Clostridium perfringens from food and animal feed, water and other materials. TSC agar with MUP can be prepared using Clostridium perfringens Supplement (1.00888,) to detect acid phosphatase by its fluorescence under UV light.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method APHA, ISO 14189, ISO 7937 Quality Level 200, form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 7.4-7.8 (25 °C in H2O) solubility 42 g/L bulk density 660 kg/m3 Featured Industry Food and Beverages storage temp. 15-25°C suitability Clostridium spp. Узнать цену -
GranuCult® VRBD (Violet Red Bile Dextrose) agar
Кат. номер 1102750500 1.10275.0500 1102755000 US11102750000 General descriptionViolet Red Bile Dextrose Agar is a granulated selective medium recommended for detection and enumeration of Enterobacteriaceae, especially the bile tolerant gram-negative bacteria from non-sterile products and pharmaceutical preparations. The granules ensure rapid and uniform dissolution in water, prevent clumping of the medium, and inhalation of the airborne powder. Pancreatic digest of gelatin and yeast extract provide nitrogenous compounds and other nutrients essential for bacterial metabolism. This media is selective due to the presence of the inhibitors, bile salts, and crystal violet. Crystal violet inhibits gram-positive organisms. The neutral red indicator helps to detect dextrose fermentation. Dextrose fermenting strains produce red colonies with pink-red halos in the presence of neutral red. Sodium chloride maintains the osmotic equilibrium in the medium.ApplicationViolet Red Bile Dextrose Agar is recommended for the detection and enumeration of Enterobacteriaceae from pharmaceutical products.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method APHA, EP, ISO 21528, JP, USP Quality Level 200, form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 7.3 (37 °C, 40 g/L in H2O, after autoclaving) solubility 40 g/L bulk density 720 kg/m3 Featured Industry Food and Beverages
Pharmaceuticalstorage temp. 15-25°C suitability enterobacteriaceae Узнать цену -
GranuCult® XLT4 agar (Base)
Кат. номер 1139190500 1.13919.0500 General descriptionGranuCult® XLT4 agar (Base) contains a selection of suitable nutrients and vitamins (peptones, yeast extract) that allow optimal growth of salmonellae. The surfactant NIAPROOF-4 (formerly Tergitol-4/Sodiumtetradecylsulfate) inhibits the accompanying flora. Salmonellae, due to H2S-formation (thiosulfate and iron (III)-ions), can be easily detected as black colonies on a red-violet background and differentiated from the residual accompanying flora. E. coli shows yellow colonies on a yellow background due to acidification of the medium (pH-indicator: phenol-red). Other accompanying organisms, like Shigella, will grow colorless on a red background due to a missing H2S-formation and acidification.ApplicationGranuCult® XLT4 agar (Base) is suitable for the isolation and identification of pathogenic Enterobacteriaceae, especially Salmonella spp.Analysis NoteAppearance (clearness): clear
Appearance (colour): red
pH-value (25°C): 7.2 - 7.6
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Salmonella anatum ATCC 9270): fair to very good
Growth (Shigella sonnei ATCC 11060): fair to very good
Growth (Shigella flexneri ATCC 12022 (WDCM 00126)): good to very good
Growth (Enterobacter aerogenes ATCC 13048): fair to good
Growth (Citrobacter freundii ATCC 8090): fair to good
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): none to fair
Growth (Proteus mirabilis ATCC 14273): none to poor
Black centre (Salmonella enteritidis ATCC 13076 (WDCM 00030)): +
Black centre (Salmonella typhimurium ATCC 14028 (WDCM 00031)): +
Black centre (Salmonella anatum ATCC 9270): +
Colony colour (Shigella sonnei ATCC 11060): colourless
Colony colour (Shigella flexneri ATCC 12022 (WDCM 00126)): colourless
Colony colour (Enterobacter aerogenes ATCC 13048): yellow
Colony colour (Citrobacter freundii ATCC 8090): yellow
Colony colour (Escherichia coli ATCC 25922 (WDCM 00013)): colourless to yellow
Incubation: 24 - 48 hrs. at 35 °C.
Base tested withXLT4 agar supplement Art. 1.08981.FootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method ISO 6579, according to USDA-FSIS Quality Level 200, description acc. USDA-FSIS and ref. to ISO 6579 GranuCult form medium granules (dehydrated (DCM)) packaging pkg of 500 g Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 7.4 (59 g/L in H2O) Featured Industry Food and Beverages storage temp. 15-25°C suitability Salmonella spp. (spp.) Узнать цену -
GranuCult® Xylose Lysine Deoxycholate agar
Кат. номер 1052870500 1.05287.0500 General descriptionGranuCult® Xylose Lysine Deoxycholate agar is designed for the isolation and differentiation of pathogenic Enterobacteriaceae, especially of Shigella and Salmonella species. Degradation of xylose, lactose, and sucrose to acid causes phenol red to change its color to yellow. Production of hydrogen sulfide is indicated by thiosulfate and iron (III) salt, which react to form a precipitate of black iron sulfide in the colonies. Bacteria that decarboxylate lysine to cadaverine can be recognized by the appearance of a purple coloration around the colonies due to an increase in pH. These reactions can proceed simultaneously or successively, causing the pH indicator to exhibit various shades of color, or it may change its color from yellow to red on prolonged incubation. The culture medium is moderately selective, thus the growth of unwanted organisms is suppressed, while sought bacteria can be tentatively grouped by reading the net effect of carbohydrate dissimilation, lysine decarboxylation, and the formation of hydrogen sulfide leading to black colonies. Agar is the solidifying agent.ApplicationGranuCult® Xylose Lysine Deoxycholate agar is suitable for the isolation and differentiation of pathogenic Enterobacteriaceae, especially of Shigella and Salmonella species from food and animal feed, water, and other materials.Analysis NoteAppearance (clearness): clear to slightly opalescent
Appearance (colour): red
pH-value (25°C): 7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)):
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)):
Colony count (Salmonella typhimurium ATCC 14028 (WDCM 00031)):
Colony count (Salmonella enteritidis ATCC 13076 (WDCM 00030)):
Recovery on test medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): 50 %
Recovery on test medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): 50 %
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): Growth or partial inhibition
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): Growth or partial inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)): Colonies with black centre
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)): Colonies with black centre
Colonies (Escherichia coli ATCC 25922 (WDCM 00013)): Yellow colonies
Colonies (Escherichia coli ATCC 8739 (WDCM 00012)): Yellow colonies
Incubation: 24 ± 3 hours at 37 ± 1 °C
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy AgarFootnoteWe offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.Legal InformationGRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Store at +15°C to +25°C. Agency/Method APHA, ISO 19250, ISO 6579 Quality Level 200, form medium granules (dehydrated (DCM)) Торговая марка GranuCult® application(s) microbiological culture: suitable, microbiology: suitable pH 7.2-7.6 (25 °C, 55 g/L in H2O) solubility 55 g/L Featured Industry Food and Beverages storage temp. 15-25°C suitability Salmonella spp. Узнать цену
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