- Производитель:
- Millipore
Кат. номер |
407207-1KIT |
407207S-1KIT |
407207-1KITCN |
• Increases signal: minimizes background resulting in high signal to noise ratios
• Versatile: can be used with nitrocellulose or PVDF membranes and is compatible with chemiluminescent or colorimetric detection systems
• Convenient: ready-to-use format, no dilution necessary
SignalBoost enhances antigen-antibody reactions, yielding signals several times to several tens of times higher than those obtained with conventional methods. It has been verified that enhancement is particularly marked for relatively inactive antibodies. Compositions optimized for primary and secondary antibodies respectively minimize background and yield high S/N ratios. It can be used for various assays involving antigen-antibody reactions, such as immunoblotting, dot blotting and ELISA. Components do not affect the activity of enzymes used for labeling (peroxidase, alkaline phosphatase, etc.), allowing use in assays using any of these enzymes. It can also be used for detection of both fluorescence and coloration. For convenience, SignalBoost is supplied in a ready-to-use form, is at an appropriate concentration for use, and does not need to be diluted. Simply dilute antibodies with this product instead of conventional diluents to use. It does not lengthen the time required for the assay.
Packaging
1 kit in Plastic ampoule
Components
Solution 1 for Primary Antibodies, Solution 2 for Secondary Antibodies, and a user protocol.
Warning
Toxicity: Standard Handling (A)
Principle
SignalBoost Immunoreaction Enhancer Solution is a reactive reagent for use in immunoassays, and was developed to resolve the problems of low sensitivity and high background often encountered during procedures such as immunoblotting and ELISA (enzyme-linked immunosorbent assay). It can be used in a range of immunoassays.
Physical form
Format: Liquid, Antibody Diluent
Storage and Stability
Upon receipt, store the entire contents of the kit at 4°C. Protect from light. Following initial use, this product is stable for up to 1 year at 4°C.
storage conditions | +2C to +8C |
usage | sufficient for 1600 cm2, sufficient for 20 miniblot(s) |
Торговая марка | Calbiochem® |
storage condition | OK to freeze, protect from light |
detection method | chemiluminescent, colorimetric, fluorometric |
shipped in | wet ice |
storage temp. | 2-8°C |
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