Human Insulin ELISA Kit RAB0327-1KT
The Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Insulin and Proinsulin in serum, plasma, cell culture supernatants and urine.
Recombinant Human Insulin
Human Insulin ELISA Kit has been used:
- to measure the blood levels of human insulin and proinsulin in the transgenic mice
- to measure serum insulin indaibetic rats
- to measure insulin in Multiple Sclerosis patients
For research use only. Not for use in diagnostic procedures.
Human Insulin ELISA Kit has been used to determine human insulin and pro-insulin concentration in serum and HAMECs (human adipose microvascular endothelial cells) which were isolated from human visceral fat of nondiabetic patients.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Insulin is responsible for two types of actions, excitatory and inhibitory. In its excitatory role, it increases the uptake of glucose and lipid synthesis, and in its inhibitory role it inhibits glycogenolysis, gluconeogenesis, lipolysis, proteolysis and ketogenesis. Insulin plays a vital role in regulation of blood glucose level within the normal range. Abnormal insulin secretion leads to various disorders such as diabetes, hyperglycemia or hypoglycemia. Type I diabetes is a result of autoimmune destruction of β cells of pancreas, which leads to depletion of insulin. Mutant INS-gene Induced Diabetes of Youth (MIDY) syndrome is an autosomal dominant disorder caused by missense mutations, which lead to aberrant proinsulin folding. Impaired glucose tolerance (IGT) or non-insulin-dependent diabetes mellitus (NIDDM) is caused by resistance to insulin-stimulated glucose uptake.
A sample Certificate of Analysis is available for this product.
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sample in the text box provided for lot number.
species reactivity | human |
packaging | kit of 96 wells (12 strips x 8 wells) |
application(s) | ELISA: suitable, capture ELISA: suitable |
input | sample type serum sample type cell culture supernatant(s) sample type urine sample type plasma |
assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 4 µIU/mL standard curve range: 4.69-300 µIU/mL |
detection method | colorimetric |
shipped in | wet ice |
storage temp. | 20°C |
Gene Information | human ... INS(3630) |
pictograms | GHS05 |
signalword | Warning |
hcodes | H290 |
RIDADR | UN 3264 8 / PGIII |