Rat/Mouse Ghrelin (total) ELISA EZRGRT-91K
eCl@ss: | 32161000 |
NACRES: | NA.32 |
Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach. Originally found as an endogenous ligand for the growth hormone secretagogue receptor, it is also an appetite-stimulating hormone in the regulation of energy homeostasis. It consists of 28 amino acids and the n-octanoylation of serine-3 position is necessary for its bioactivity. The acylated form is extremely unstable in the blood; consequently, the predominant form is des-octanoyl ghrelin. Long considered a key player in obesity, ghrelin may also play a role in maintaining the balance between insulin and glucose and the development of diabetes. The spike in insulin secretion occurring after eating usually correlates with a dip in ghrelin production. Researchers determined that in rat models of uncontrolled diabetes, food intake still suppressed ghrelin concentrations in the blood, but only approximately half as effectively as when insulin was present.
Specificity
Canine Ghrelin (Active) - 71%
Human Ghrelin (Active) - 115%
Human Des-Octanoyl Ghrelin - 249%
Porcine Ghrelin (Active) - 48%
Rat/Mouse Ghrelin (Active) - 85%
Rat/Mouse Des-Octanoyl Ghrelin - 100%
Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)
Research Sub Category
Obesity
Metabolic Disorders
Research Category
Metabolism
Used to detect/quantify: Ghrelin (Total)
Please contact Technical Service for linearity of dilution.
Disclaimer
For research use only. Not for use in diagnostic procedures.
Quality Level | 100 |
species reactivity | rat, mouse |
assay range | accuracy: 80-103% (Serum: Active Ghrelin)","accuracy: 86-102% (Plasma: Active Ghrelin)","accuracy: 92-103% (Serum: Des-octanoyl Ghrelin)","accuracy: 95-107% (Plasma: Des-octanoyl Ghrelin)","inter-assay cv: 1.8-4.5% intra-assay cv: 0.7-1.3% (Serum)","inter-assay cv: 2.0-3.2% intra-assay cv: 1.1-1.7% (Plasma) |
application(s) | ELISA: suitable |
UniProt accession no. | Q9EQX0 (mouse),","Q9QYH7 (rat),","Q9UBU3, |
detection method | colorimetric","fluorometric |
pictograms | GHS05,GHS06,GHS09 |
signalword | Danger |
pcodes | P273 - P280 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P311 |