Packaging
1 kit in 96 wells
96 wells
Used to detect/quantify: Phosphorylated Neurofilament (pNF-H)
Research Category
Neuroscience
The Phosphorylated Neurofilament H (pNF-H) kit is a sandwich enzyme immunoassay (EIA), which measures pNF-H.
Components
Immunoplate Pre-coated with Chicken anti-pNF-H Polyclonal Antibody: (Part No. 2006263). One 96-well immunoplate pre-coated with chicken anti- pNF-H polyclonal antibody sealed in a foil pouch.
Rabbit anti- pNF-H Polyclonal Antibody: (Part No. 2006919). One vial (100 µL) of rabbit anti- pNF-H polyclonal antibody.
Goat anti-Rabbit, Alkaline Phosphatase Conjugated Antibody: (Part No. 2006917). One vial (10 µL) of goat anti-rabbit alkaline phosphatase conjugate.
Bovine pNF-H Standard: (Part No. 2006918). One vial (5.0 ng) of bovine pNF-H standard.
20X TBS: (Part No. 2004913). One bottle (50 mL) of 20X TBS.
20% Tween-20® : (Part No. 2004915). One vial (3 mL) of 20% Tween-20.
Elisa Assay Diluent: (Part No. 2006921) One bottle (30 mL) of Elisa assay diluent containg 0.05% Kathon as a preservative, provided in a ready to use format.
pNPP Buffer: (Part No. 2006261). One bottle (12 mL) of pNPP buffer for use with 50X p-NPP, provided in a ready to use format.
50X pNPP: (Part No. 2006262). One vial (250 µL) of 50X pNPP (p-nitrophenyl phosphatase) alkaline phosphatase substrate.
Plate Covers - Two Plate Covers.
Storage and Stability
Prior to use maintain the kit components at 2°-8°C and -20°C, as indicated, until expiration date.
1. Wash Buffer - After dilution maintain the Wash Solution at 2°-8°C for up to one month or until expiration date.
2. Tween-20® - Store at room temperature upon receipt of kit.
3. Elisa Assay Buffer - Aliquot as required to avoid repeated freeze-thaw cycles. After thawing, maintain the Elisa Assay Buffer at 2°-8°C for up to one month, or until expiration date.
4. Immunoplate Pre-coated with Chicken anti- pNF-H Polyclonal Antibody - Unused strip wells may be returned to the foil pouch and stored at 2°-8°C for up to one month, or until expiration date.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Disclaimer
For research use only. Not for use in diagnostic procedures.
Quality Level | 100 |
species reactivity | feline, bovine, pig, rat, mouse, human |
Торговая марка | Chemicon® |
assay range | sensitivity: 0.0293-15 ng/mL (Range of Detection)","sensitivity: 0.0585 ng/mL |
application(s) | ELISA: suitable |
NCBI accession no. | NM_021076.2, |
UniProt accession no. | P12036, |
detection method | colorimetric |
shipped in | dry ice |
pictograms | GHS05,GHS08 |
signalword | Danger |
pcodes | P280 - P302 + P352 - P305 + P351 + P338 - P314 |