Tumor necrosis factor-α (TNF-α) is encoded by the gene mapped to human chromosome 6p21.3, within the major histocompatibility complex (MHC) class III region. The encoded protein belongs to the TNF superfamily.
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1 alpha, MIP-1 beta, NAP-2, PDGF, PF-4
Please refer to the attached Protocol,for details.
Human tumor necrosis factor-alpha (TNF-) ELISA Kit has been used in enzyme-linked immunosorbent assay (ELISA) to quantify TNF- in supernates of human acute lung infection model (ALIM), human umbilical vein endothelial cells (HUVEC) and human microvascular endothelial cells (HMEC-1).
Biochem/physiol Actions
Tumor necrosis factor-α (TNF-α) is a key immunomodulator and proinflammatory cytokine. It is implicated in the immune pathogenesis of various autoimmune, inflammatory diseases and hepatitis B virus (HBV) infection. Mutation in the gene increases the risk of susceptibility to diffuse large B-cell lymphoma (DLBCL) and marginal zone lymphoma. In addition, polymorphism in the gene leads to obsessive-compulsive disorder (OCD) and osteoarthritis (OA).
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species reactivity | human |
packaging | kit of 96 wells (12 strips x 8 wells) |
application(s) | ELISA: suitable |
input | sample type cell lysate sample type tissue lysate |
assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 30 pg/ml |
detection method | colorimetric |
shipped in | wet ice |
storage temp. | 20°C |
Gene Information | human ... TNF(7124) |
pictograms | GHS05 |
signalword | Warning |
hcodes | H290 |
RIDADR | UN 3264 8 / PGIII |
Flash Point F | Not applicable |
Flash Point C | Not applicable |