ECM Gel from Engelbreth-Holm-Swarm murine sarcoma

MDL number:	MFCD00283131
NACRES:	NA.75

Кат. номер

E1270-10ML-PW

E1270-1ML-PW

E1270-5ML-PW

E1270-1ML

E1270-5ML

E1270-1ML-KC

E1270-10ML

E1270-5X10ML

ECM gel can used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells.
Biochem/physiol Actions
The ECM gel will undergo thermally activated polymerization when brought to 20-40°C, forming a reconstituted basement membrane. PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel.
Components
ECM was prepared to a protein concentration of 8-12 mg/mL (in DMEM), containing laminin as a major component, collagen type IV, heparin sulfate proteoglycan, entactin, and other minor components. Addition of collagen type IV to the ECM gel increases polymerization.
Product contains 50 mg/L Gentamicin Sulfate.
Caution
For long term storage, keep product at -20°C. The ECM gel may be stored at 2-8°C for up to 72 hours.
Preparation Note
This product is prepared from Engelbreth-Holm-Swarm sarcoma in mice; dialyzed against chloroform. Gel should be thawed overnight at 2-8°C before use and dispensed to a multiwall plate using a plate and pipettes that are pre-cooled to 2-8°C. The gel may also be diluted up to twofold with 2-8°C Dulbeccos Modified Eagles Medium, and should be done before gel is added to the plate. The product will gel within 5 minutes at 20°C. For prolonged manipulations, work should be conducted below 10°C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium, magnesium, or EDTA, at a concentration of 0.6-2.4 units per mL.

Quality Level	300
product line	BioReagent
form	liquid
concentration	8-12 mg/mL
application(s)	cell culture | mammalian: suitable
UniProt accession no.	P02468,","P02469,","P10493,","P19137,","P43407,","Q61001,","Q61092,","Q61789,
shipped in	dry ice
storage temp.	20°C
Gene Information	mouse ... Lama1(16772), Lama3(16774), Lama5(16776), Lamb1-1(16777), Lamc1(226519), Lamc2(16782), Nid1(18073), Sdc2(15529)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable