WNT-1, Human Recombinant GF175

eCl@ss:	32160405
NACRES:	NA.75

Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, β-catenin. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.
Product Source: E. coli.
Specificity
Cross Reactivty
Mouse
Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors
Quality
Bioactivity assay: The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
Physical form
Product is filtered through a 0.2 micron filter before lyophilization. Product is presented in .
Storage and Stability
Store at -20°C for up to 6 months from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
assay	>98% (SDS-PAGE and HPLC)
application(s)	cell culture | stem cell: suitable
impurities	<0.1 µg/mg protein endotoxin (<1EU/µg)
UniProt accession no.	P04628,
shipped in	dry ice

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable