Tumor Necrosis Factor- from mouse

MDL number:	MFCD00164823
NACRES:	NA.77

Кат. номер

T7539-50UG-PW

T7539-10UG

T7539-50UG

T7539-10UG-PW

Tumor Necrosis Factor- from mouse, a potent proinflammatory cytokine has been used:

• to activate MS1 endothelium cells
• to induce lipolysis in adipocytes
• to study its effect on liganded and unliganded glucocorticoid receptor (GR) in interleukin (IL)-6 and IL-8 gene regulation

Biochem/physiol Actions
Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated.
Unit Definition
One unit is the amount required to induce half-maximal cytolysis of L929 cells.
Physical form
Lyophilized from 10 mM sodium phosphate, pH 7.5

Quality Level	200
biological source	mouse
recombinant	expressed in E. coli
assay	95%
form	powder
potency	0.01-0.5 ng/mL EC50
mol wt	17 kDa
packaging	pkg of 10 µg","pkg of 50 µg
storage condition	avoid repeated freeze/thaw cycles
application(s)	cell culture | mammalian: suitable
impurities	1 EU/µg protein endotoxin
color	white
solubility	water: soluble
UniProt accession no.	P06804,
storage temp.	20°C
Gene Information	mouse ... Tnf(21926)

pictograms	GHS07
signalword	Warning
hcodes	H315 - H319
pcodes	P305 + P351 + P338
Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable