TPO,Human Recombinant Animal Free GF347

eCl@ss:

32160405

Product Source: Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.
TPO, or thrombopoietin, is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity.
Recombinant human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein.
Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors
Quality
Determined by its ability to stimulate the proliferation of human MO7e cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10^6 units/mg.
Physical form
Product is presented in 0.1% TFA and is filtered through a 0.2 micron filter before lyophilization.
Storage and Stability
Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

impurities	<0.1 µg/mg protein endotoxin (<1 EU/µg)
Quality Level	100
UniProt accession no.	P40225,
shipped in	dry ice

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable