RANK Ligand from mouse

MDL number:	MFCD01867142
NACRES:	NA.32

Кат. номер

R0525-10UG-PW

R0525-10UG

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or receptor activator of nuclear factor-κB ligand (RANKL) is a type II transmembrane signaling receptor. It has a molecular weight of about 35kDa. The protein possesses an amino-terminal intracellular tail and a carboxy-terminal extracellular region, which has a connecting stalk and a receptor-binding domain.
RANK ligand from mouse has been used to visualize differentiated osteoclasts and to stimulate osteoclast differentiation.
The functions of RANK Ligand include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function induction of osteoclastogenesis, and lymph node organogenesis.The cell surface signaling receptor of RANK Ligand is RANK, which undergoes receptor clustering during signal transduction.
Biochem/physiol Actions
Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or RANKL has an important role in osteoclast function and differentiation. It binds to RANK and osteoprotegrin (OPG). Bioactivities of RANKL may be attributed to its binding to these two receptor molecules. Mouse and human RANKL share 85% amino acid sequence identity.
Physical form
Lyophilized from a 0.2 μm filtered solution of 5 mM sodium phosphate, pH 7.6, and 75 mM NaCl.
Analysis Note
The bioactivity is determined by its ability to induce osteoclast differentiation of RAW 264.7. The expected ED50 for this effect is 10 ng/ml.

Quality Level	200
biological source	mouse
recombinant	expressed in E. coli
assay	98% (SDS-PAGE)
form	lyophilized powder
potency	10.0-25.0 ng/mL ED50
packaging	pkg of 10 µg
storage condition	avoid repeated freeze/thaw cycles
application(s)	cell culture | mammalian: suitable
impurities	<0.1 ng/mg","<1 EU/µg
color	white
UniProt accession no.	O35235,
storage temp.	20°C
Gene Information	mouse ... Tnfsf11(21943)

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable