Nerve Growth Factor-7S from murine submaxillary gland

CAS Number:	93928-24-6
EC Number:	210-123-3
MDL number:	MFCD00147703
NACRES:	NA.77

Кат. номер

N0411-1VL-PW

N0513-PH

N0513-.1MG

Nerve growth factor (NGF) is a dimer of 118-residue polypeptide chains linked non-covalently. Both these polypeptides contain three intramolecular disulphide bridges. It forms the prototype of the neurotrophin growth factor family. In male mouse submandibular salivary gland, NGF is present in high amounts, and is present as a high molecular weight complex called nerve growth factor-7S (NGF-7S). NGF-7S is an 222 complex, where 2-NGF is the active neurotrophin, and is linked to the dimers of the glandular kallikrein serine proteinases members, -NGF and -NGF.
Nerve Growth Factor-7S (NGF-7S) from murine submaxillary gland has been used for the treatment of following cells to induce neuronal differentiation-

• PC12
• Dorsal root ganglia (DRG) obtained from chick embryos of either sex (Gallus gallus)

Biochem/physiol Actions
Nerve growth factor (NGF) is a neurotrophin, which facilitates the differentiation and survival of specific subtypes of neurons of central nervous system and peripheral nervous system. However, nerve growth factor-7S (NGF-7S) lacks any neurotrophic activity as most part of β-NGF subunit is buried within the NGF-7S complex.
Physical form
Lyophilized from 0.2 μm filtered solution in 25 mM sodium phosphate, pH 7.0.
Preparation Note
Nerve growth factor-7S (NGF-7S) is isolated from male mouse submaxillary glands using a modification of the method of Varon, et al.
Analysis Note
The biological activity is measured using a 3-day MTT assay.

Quality Level	200
biological source	mouse
form	lyophilized powder
potency	2-250 ng/mL ED50/EC50
quality	endotoxin tested
mol wt	protein 130 kDa
packaging	pkg of 0.1 mg
application(s)	cell culture | mammalian: suitable
impurities	10
color	white
UniProt accession no.	P01139,
storage temp.	20°C
Gene Information	mouse ... Ngf(18049)

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable