Insulin-like Growth Factor-I human

MDL number:	MFCD00081716
NACRES:	NA.77

Кат. номер

I3769-100UG

I3769-50UG-PW

I3769-50UG

I3769-100UG-PW

Insulin-like growth factor I (IGF-I) is localized on human chromosome 12q23.2.
Insulin-like Growth Factor-I human has been used:

• in modified Dulbecco minimal essential medium for in vitro culture of testicular cells
• in culture media, to study its effect on steroidogenesis during testicular development
• in culture media for in vitro assay of splenic pro-erythroid cell growth and differentiation

Biochem/physiol Actions
IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. In the developing embryo IGF-I is expressed primarily by mesenchymal-derived cells. After birth IGF-I expression in most extrahepatic tissues declines and hepatic expression of IGF-I becomes GH-regulated. Expression of IGF-I outside the liver is regulated differently, depending on the specific tissues. For example, gonadotropins and sex steroids regulate IGF-I expression within the reproductive system, while parathyroid hormone and sex steroids regulate IGF-I expression in bone. IGF-I is produced in several human tumors. IGF-I is mitogenic for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis.
Insulin-like growth factor I (IGF-I) plays a critical role in cellular energy metabolism and growth and development, especially prenatal growth.
Physical form
Lyophilized from 0.2 μm filtered solution in 50 mM acetic acid.
Reconstitution
Reconstitute in water
Analysis Note
The biological activiy is calculated by the dose-dependent proliferation of murine BALBC/3T3 cells. ED50 corresponds to a specific activity of at least 1 x 105 units/mg.

Quality Level	200
biological source	human
recombinant	expressed in E. coli
assay	95% (HPLC)
form	lyophilized powder
potency	0.1-10 ng/mL ED50/EC50
quality	endotoxin tested
mol wt	~7.6 kDa
packaging	pkg of 50 and 100 µg
application(s)	cell culture | mammalian: suitable
impurities	1.00 EU/µg
UniProt accession no.	P05019,
storage temp.	20°C
Gene Information	human ... IGF1(3479), Igf1(16000)

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable