- Производитель:
- Sigma-Aldrich
CAS Number: | 62253-63-8 |
MDL number: | MFCD00132565 |
NACRES: | NA.77 |
Кат. номер |
E9644-.1MG-PW |
E9644-.2MG-PW |
E9644-.5MG-PW |
E9644-.5MG |
E9644-5X.2MG |
E9644-.2MG |
E9644-BR |
Packaging
0.2 mg in glass bottle
Biochem/physiol Actions
Epidermal Growth Factor (EGF) is a small mitogenic polypeptide present throughout a large number of tissues and body fluids in many mammalian species. EGF is a member of a growth factor family characterized by 6 conserved cysteine motifs that form three disulfide bonds. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, vascular and corneal endothelial cells, bovine granulosa, HeLa cells, SV40-3T3 cells and mammary epithelial cells.
Cellular functions affected by EGF: mitosis, ion flux, glucose transport, glycolysis, nucleic acid and protein synthesis, survival, growth, proliferation and differentiation.
Biological affects of EGF: inhibition of gastric acid secretion, fetal growth and development and neuromodulation in the central nervous system.
Pathways affected by EGF: EGFR signaling, MAPK cascade, PIP, Ca+2 signaling
Components
Human EGF (hEGF) is an identical molecule to β-urogastrone, a molecule isolated on the basis of its ability to inhibit gastric acid secretion. EGF is structurally homologous to human transforming growth factor-α, and both exert their actions through EGF receptors. E9644 is the N-terminal methionyl form of natural mature EGF.
Caution
This product should be stored at -20°C and will retain activity for two years. After reconstitution, it can be stored at 2-8°C for one month, or frozen in aliquots at -70°C or -20°C for longer periods.
Preparation Note
This product was lyophilized from a 0.2 μm-filtered solution of phosphate buffered saline, at pH 7.4. It should be reconstituted by adding the contents of the vial to 1 mg/mL using 10 mM acetic acid. To dilute to lower concentrations, but no fewer than 10 μg/mL, an addition of 0.1% BSA or HSA will be needed. For use in proliferation assays, further dilute the sample with medium without albumin.
Analysis Note
The biological activity of this product is measured in a proliferation assay using BALB/MK cells. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.
Quality Level | 300 |
biological source | human |
recombinant | expressed in E. coli |
assay | 97% (SDS-PAGE) |
form | lyophilized powder |
potency | 0.08-0.8 ng/mL ED50/EC50 |
mol wt | ~6 kDa |
packaging | pkg of 5X0.2 mg","pkg of 0.2 mg","pkg of 0.5 mg |
storage condition | avoid repeated freeze/thaw cycles |
application(s) | cell culture | mammalian: suitable |
impurities | 1 EU/µg endotoxin (EGF) |
color | white |
solubility | water: soluble, clear, colorless |
UniProt accession no. | P01133, |
storage temp. | 20°C |
Gene Information | human ... EGF(1950) |
Personal Protective Equipment | Eyeshields,, Gloves,, type N95 (US), |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point F | Not applicable |
Flash Point C | Not applicable |
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