Erythropoietin, human (hEPO) 11120166001

Recombinant, Erythropoietin, human (hEPO), is produced in CHO cells (chinese hamster ovary) and purified by standard chromatographic techniques. The glycoprotein encoded by erythropoietin (EPO) is synthesized in kidneys. It is the principal hormone that has a short half-life. Cells responsive to EPO have been identified in adult bone marrow, fetal liver, or adult spleen.
Detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Specificity
Human Erythropoietin (hEPO) is effective on mouse and human cells.
Erythropoietin (EPO) is a glycoprotein which stimulates proliferation and differentiation of erythroid precursor cells (CFU-E, BFU-E) to more mature erythrocytes.
Biochem/physiol Actions
Erythropoietin (EPO) controls the synthesis of RBCs (red blood cells) by inducing the proliferation and differentiation of erythroid precursor cells. As it induces erythropoiesis, EPO can be used to treat anemia of cancer patients.
Sequence
One polypeptide chain (166 amino acids), glycosylated, identical to natural hEPO. The carbohydrate structure of recombinant EPO isolated from CHO cells is very similar to that of natural EPO.
Unit Definition
EC50 definition: The amount of hEPO that is required to produce equivalent [3H]-thymidine incorporation into spleen cells from phenylhydrazine-treated mice to that expressed by 1 unit of the WHO-EPO reference standard (2nd IRP) (1 unit equals 10 ng).
Physical form
Solution, filtered through 0.2 μm pore size membrane.
Preparation Note
Working solution: Dilute the concentrated EPO solution (250 U/ml) with PBS or culture medium containing 1 mg/ml (0.1%) BSA (or HSA) or 1 to 10% serum.
Storage conditions (working solution): -15 to -25 °C
Store the solution in aliquots at -15 to -25 °C
Note: Avoid repeated freezing and thawing
For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	human
recombinant	expressed in CHO cells Hamster
assay	>98% (HPLC and SDS-PAGE)
form	solution
packaging	pkg of 250 U (2.5 µg, 1 ml)
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
impurities	<1 EU/µgtested (LAL test)
UniProt accession no.	P01588,
storage temp.	20°C
Gene Information	human ... EPO(2056)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available